Tie2 cre transgenic mice

The Col1α1-GFP/Tie2-Cre/Rosa-tdTomato DRM were generated by crossing Col1α1-GFP transgenic mice (Jackson Laboratory #013134), Tie2-Cre transgenic mice (Jackson Lab #004128), and Rosa-tdTomato (RFP) mice (Jackson Laboratory #007914). Mice were the C57BL/6 strain. The Rosa-tdTomato mice were first bred with female Tie2-Cre mice to generate Tie2-Cre/Rosa-tdTomato (RFP reporter) mice. Female RFP reporter mice were mated with Col1α1-GFP mice to generate DRM mice. All the mouse strains and other key reagents used in this study are listed in key resources table. Primer sequences used are listed in Table S2.
Mouse manipulations were approved by the MD Anderson Cancer Center Institutional Animal Care and Use Committee.

Tie2-Cre transgenic mice were obtained from The Jackson Laboratory (Bar Harbor, ME). We generated endothelial-specific Gclc knockout [Gclc(e/+)] mice by intercrossing Gclc(f/f) mice with Tie2-Cre transgenic mice. The floxed Gclc mouse line, described in [25] (link) was provided under agreement by Professors Ying Chen and Vasilis Vasiliou, from the University of Colorado. We conducted all studies on littermates. Animals were handled in agreement with the Guide for the Care and Use of Laboratory Animals contained in Directive 2010/63/EU of the European Parliament.