Expanded graphite powder (100 mesh) was purchased from Xinghe Graphite Co., Ltd. (Qingdao, China). Chemicals used in the synthesis of GO were purchased from Beijing Chemical Works (Beijing, China). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 2-(N-morpholino)ethanesulfonic acid (MES) were purchased from the J&K (Beijing, China). Bovine serum albumin (BSA) and human α-thrombin were purchased from Sigma-Aldrich. The aptamer targeting human α-thrombin with a five T spacer (5′-TTTTT GGT TGG TGT GGT TGG-3′, named as Apt-15), with the 5′ end modified by the amino group through a C6-carbon spacer arm, was synthesized by Sangong Biotech (Beijing, China). Other chemicals and analytically pure reagents were used as received. All solutions were prepared with deionized water.
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc
Manufactured by J&K Scientific
Sourced in China
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) is a water-soluble carbodiimide used in chemical synthesis. It is commonly employed as a coupling agent to facilitate the formation of amide bonds between carboxylic acids and primary amines.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide nhs, by J&K Scientific (3 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Human α thrombin, by Merck Group (1 mentions)
Nafion, by Merck Group (1 mentions)
Chitosan, by Thermo Fisher Scientific (1 mentions)
D glucose, by Sinopharm (1 mentions)
Dopamine hydrochloride, by Merck Group (1 mentions)
4 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc
1
Synthesis of Graphene Oxide for Thrombin Aptamer Immobilization
2
Enzymatic Glucose Biosensor Fabrication
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Polyacrylamide solution (average Mw = 10,000, 50 wt % in H2O) and Nafion (product No. 274704) were purchased from Aldrich (Shang Hai, China). All aqueous solutions were prepared with deionized water (18 MΩ·cm−1) from a Millipore system. GOx and chitosan were purchased from Alfa Aesar (Shang Hai, China). . D-(+)-Glucose was bought from Sinopharm Chemical Reagent Co., Ltd. (Shang Hai, China). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were obtained from J&K Scientific (Shang Hai, China). We used commercial compressed air from a cylinder to maintain the oxygen concentration during electrochemical experiments. All other reagents were analytical grade and used without further purification. All chemicals were stored in a 4 °C refrigerator until used.
3
Preparation of Amino-Modified FMT Nanoparticles
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FMT synthesis has been described in previous reports 45 (link), 46 . The procedures used to prepare the amino-modified FMT are as follows. FMT nanoparticles (100 mg, containing carboxyl groups on the surface) were first dissolved in a solution of 100 mL dimethyl sulfoxide containing 12 mg of dimethylaminopyridine (DMAP, 0.1 mmol) (Sigma-Aldrich, St. Louis, MO), and were then added to 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (J&K Scientific Ltd, Shanghai, China) (192 mg, 1 mmol), and N-hydroxysuccinimide (NHS) (J&K Scientific Ltd) (115 mg, 1 mmol). The resulting mixture was then stirred at room temperature for 2 h and 1, 3-diaminopropane (J&K Scientific Ltd) (150 mg, 2 mmol) was added to the mixture and stirred for another 24 h. Thereafter, the reaction mixture was added with excess acetone to obtain crude FMT-NH2 nanoparticles. The crude products were washed thrice with acetone and dried under reduced pressure.
4
Synthesis of Dopamine-PEG Conjugates
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Dopamine hydrochloride (DA) was purchased from Sigma-Aldrich Co. Pyrrole (PY), ethanol, and ammonia (28–32%) were obtained from Sinopharm Chemical Reagent Co., Ltd., (Shanghai, China), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from J&K Scientific Ltd. (Beijing, China). Amine-PEG-Amine (NH2-PEG-NH2, 6 kDa, ≥99.0%) was purchased from Chemgen Pharma Co., Ltd. (West Bengal, India), and 2,2-biphenyl-1-picrylhydrazyl (DPPH) was purchased from Aladdin Co., Ltd (Shanghai, China). All reagents were used directly without further purification. MCF-7, Hela and HUVEC cell lines were purchased from the Wuhan Cell bank of Chinese Academy of Sciences.
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