M8042

Manufactured by Merck Group

Sourced in United States, Germany

The M8042 is a laboratory equipment product offered by Merck Group. It is designed for performing various scientific procedures and experiments within a controlled laboratory environment. The core function of the M8042 is to facilitate precise measurements and data collection for research and analytical purposes. However, a detailed description of the product's specifications and intended use cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

9 protocols using m8042

Culturing Diverse Breast Cancer Cell Lines

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4T1 murine breast cancer cells, ZR-75-1 human breast cancer cells were maintained in RPMI-1640 (Sigma-Aldrich, R5886) medium containing 10% FBS, 1% penicillin/streptomycin, 2 mM L-glutamine and 1% pyruvate at 37 °C with 5% CO₂.
MDA-MB-231 and SK-BR-3 human breast cancer cells were maintained in DMEM (Sigma-Aldrich, 1000 mg/l glucose, D5546) containing 10% FBS, 1% penicillin/streptomycin, 2 mM L-glutamine and 10 mM HEPES at 37 °C with 5% CO₂.
MCF7 human breast cancer cells were maintained in MEM (Sigma-Aldrich, M8042) medium containing 10% FBS, 1% penicillin/streptomycin, 2 mM L-glutamine and 10 mM HEPES at 37 °C with 5% CO₂.
Human primary fibroblast cells were maintained in DMEM (Sigma-Aldrich, 1000 mg/l glucose, D5546) containing 20% FBS, 1% penicillin/streptomycin, 2 mM L-glutamine and 10 mM HEPES at 37 °C with 5% CO₂.

Kovács T., Mikó E., Vida A., Sebő É., Toth J., Csonka T., Boratkó A., Ujlaki G., Lente G., Kovács P., Tóth D., Árkosy P., Kiss B., Méhes G., Goedert J.J, & Bai P. (2019). Cadaverine, a metabolite of the microbiome, reduces breast cancer aggressiveness through trace amino acid receptors. Scientific Reports, 9, 1300.

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Culturing Diverse Breast Cancer Cell Lines

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The 4T1 murine breast cancer cells were maintained in RPMI-1640 (Sigma-Aldrich, R5886) medium containing 10% FBS, 1% penicillin/streptomycin, 2 mM L-glutamine, and 1% pyruvate at 37 °C with 5% CO₂. MCF7 human breast cancer cells were maintained in MEM (Sigma-Aldrich, M8042) medium containing 10% FBS, 1% penicillin/streptomycin, and 2 mM L-glutamine at 37 °C with 5% CO₂. SKBR-3 human breast cancer cells were maintained in DMEM (Sigma-Aldrich, 1000 mg/L glucose, D5546) medium containing 10% FBS, 1% penicillin/streptomycin, and 2 mM L-glutamine at 37 °C with 5% CO₂. ZR75-1 human breast cancer cells were maintained in RPMI-1640 (Sigma-Aldrich, R5886) medium containing 10% FBS, 1% penicillin/streptomycin, and 2 mM L-glutamine at 37 °C with 5% CO₂. Human primary fibroblasts cells were maintained in DMEM (Sigma-Aldrich, 1000 mg/L glucose, D5546) medium containing 20% FBS, 1% penicillin/streptomycin, and 2 mM L-glutamine at 37 °C with 5% CO₂.

Sári Z., Mikó E., Kovács T., Boratkó A., Ujlaki G., Jankó L., Kiss B., Uray K, & Bai P. (2020). Indoxylsulfate, a Metabolite of the Microbiome, Has Cytostatic Effects in Breast Cancer via Activation of AHR and PXR Receptors and Induction of Oxidative Stress. Cancers, 12(10), 2915.

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Culturing Human Embryonal Kidney Cells

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TSA201 (Human Embryonal Kidney) cells were grown in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM, M8042, Sigma-Aldrich, St. Luis, MO, USA) in the presence of 10% Fetal Bovine Serum (FBS, F9665, Sigma-Aldrich and 1.5% L-glutamine (G7513-100ML, Sigma-Aldrich, St. Luis, MO, USA). The media of overexpressed cell lines contained 10 μg/mL puromycin. Cells were maintained in T-75 culture flasks at 37 °C in 5% CO₂ atmosphere.

Sipos Á., Szennyes E., Hajnal N.É., Kun S., Szabó K.E., Uray K., Somsák L., Docsa T, & Bokor É. (2021). Dual-Target Compounds against Type 2 Diabetes Mellitus: Proof of Concept for Sodium Dependent Glucose Transporter (SGLT) and Glycogen Phosphorylase (GP) Inhibitors. Pharmaceuticals, 14(4), 364.

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Preosteoblast Viability Assessment Protocol

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Preosteoblasts (MC3T3-E1 Subclone 4, ATCC CRL-2593) were cultured in MEM α (M8042, Sigma, St. Louis, MO, USA) supplemented with 10% Fetal Bovine Serum (FBS, F7524, Sigma, St. Louis, MO, USA), 1% L-glutamine and 1% Penicillin-Streptomycin (P4333, Sigma) at 37 °C, 5% CO₂. The cells were seeded at a density of 3 × 10⁵ cells/cm² on investigated materials, left to adhere for 20 min, and maintained for 24 h in the culture medium.
The cellular viability was evaluated with calcein-AM (LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells, L3224, Life Technologies, Waltham, MA, USA) and propidium iodide. After 24 h in culture, the samples were washed with 1× Phosphate Buffered Saline (PBS, P3813, Sigma), and incubated for 40 min at room temperature with 1 µM calcein-AM and 1 µg/mL propidium iodide. Viability was assessed by fluorescence microscopy with a Zeiss LSM 880 confocal system (Zeiss, Oberkochen, Germany) with 488 and 514 nm lasers, and images were processed with ZEN 2.3 software (Zeiss, Oberkochen, Germany).

Busuioc C., Isopencu G., Banciu A., Banciu D.D., Oprea O., Mocanu A., Deleanu I., Zăuleţ M., Popescu L., Tănăsuică R., Vasilescu M, & Stoica-Guzun A. (2022). Bacterial Cellulose Hybrid Composites with Calcium Phosphate for Bone Tissue Regeneration. International Journal of Molecular Sciences, 23(24), 16180.

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CHO DG44 Cell Culture and Transfection

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CHO DG44 cells, obtained from Dr. Lawrence Chasin at Columbia University, were maintained in α-MEM medium with nucleotide (Sigma, M8042) supplemented with 10% fetal bovine serum (FBS), as previously described [8] (link), [14] (link). Plasmid DNA was transfected into cells using Lipofectamine 2000 reagent (Invitrogen) and Opti-MEM (Gibco). One day after transfection, 5 µg/ml of blasticidine was added to select for transformants, and the concentration was increased to 10 µg/ml on day 10. Usually, on day 23, the medium was replaced with α-MEM without nucleotide (Sigma, M4526) supplemented with 10% dialyzed FBS (Thermo Scientific). Methotrexate (Sigma) was dissolved in water and added to the culture at 5 or 50 nM. Cloning of cells was performed by limiting dilution.

Tanaka S.S., Mitsuda S.H, & Shimizu N. (2014). How a Replication Origin and Matrix Attachment Region Accelerate Gene Amplification under Replication Stress in Mammalian Cells. PLoS ONE, 9(7), e103439.

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EV-depleted Fetal Calf Serum Generation

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For the generation of EV-depleted FCS, FCS (fetal calf serum) (#F7524, Sigma-Aldrich GmbH; Germany) was diluted in α-MEM (#M8042, Sigma-Aldrich GmbH; Germany) medium to a final 20% concentration and subjected to sequential ultracentrifugation steps at 120,000×g (L-90 K, Beckman Coulter, Brea; USA) at 4 °C for 18 h [34 ]. EVs depleted FCS (FCS^depl-uc) was stored at − 20 °C in α-MEM for use in cell culture experiments.

Li S., Stöckl S., Lukas C., Herrmann M., Brochhausen C., König M.A., Johnstone B, & Grässel S. (2021). Curcumin-primed human BMSC-derived extracellular vesicles reverse IL-1β-induced catabolic responses of OA chondrocytes by upregulating miR-126-3p. Stem Cell Research & Therapy, 12, 252.

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Depletion of FCS for EV Culture

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In order to deplete the FCS from EVs, FCS was diluted in α-MEM medium (M8042, Sigma-Aldrich, Germany) to different concentrations (20, 50, and 100%) and subjected to ultracentrifugation at 120,000 × g (L-90 K, Beckman Coulter, Brea; USA) at 4°C for 18 h. Phosphate-buffered saline (PBS), α-MEM, and commercially available EV-depleted FCS (FCS^depl-com #A27208-03, GIBCO, USA) were used as controls. Depletion efficacy was analyzed using western blotting. The comparability of controls and FCS, depleted via ultracentrifugation (FCS^depl-uc), was verified via proliferation (BrdU incorporation) and apoptosis (caspase 3/7 activity) assays with hBMSC after being cultured for 24 h in the presence of controls and FCS^depl-uc.

Li S., Stöckl S., Lukas C., Götz J., Herrmann M., Federlin M, & Grässel S. (2020). hBMSC-Derived Extracellular Vesicles Attenuate IL-1β-Induced Catabolic Effects on OA-Chondrocytes by Regulating Pro-inflammatory Signaling Pathways. Frontiers in Bioengineering and Biotechnology, 8, 603598.

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Neuronal Differentiation of Neuro2A and P19 Cells

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Neuro2A (N2A, ATCC #CCL-131) cells were grown at 37°C, 5% CO₂, and 95% humidity in DMEM (Sigma-Aldrich #D6429), 10% fetal bovine serum (FBS, Sigma-Aldrich #12306C). Cells were plated on Greiner Bio-One Advanced TC 35- or 60-mm plates. Cells were split at 1:5 or 1:10 every 48 h. Differentiation was initiated by plating the cells in DMEM, 2% FBS, 20 µM all-trans retinoic acid (ATRA, Sigma-Aldrich #R2625). Cells were harvested after 48 h by simple pipetting.
P19 cells (ATCC #CRL-1825) were cultured in α-MEM (Sigma-Aldrich # M8042), 10% FBS (Sigma-Aldrich #12306C) at 37°C, 5% CO₂, and 95% humidity. P19 cells were passaged every 48 h at 1:5 or 1:10. Cells were differentiated using α-MEM growth medium, 10% FBS, 0.1% dimethyl sulfoxide (Sigma-Aldrich #D2438). Cells were harvested after 48 h by simple pipetting.

Onderak A.M, & Anderson J.T. (2017). Loss of the RNA helicase SKIV2L2 impairs mitotic progression and replication-dependent histone mRNA turnover in murine cell lines. RNA, 23(6), 910-926.

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Crocin Modulation of LPA-Induced Macrophages

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Mouse RAW264.7 macrophages (CL-0190, Procell, Wuhan, China) were normally cultured in α-minimum essential medium (α-MEM, M8042, Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (FBS, F2442, Sigma-Aldrich, USA) and 1% penicillin-streptomycin (P4333, Sigma-Aldrich, USA) at 37°C with 5% CO₂.
For the construction of in vitro cell models, crocin was administered simultaneously with LPA in the in vitro model. RAW264.7 cells were cultured in the α-MEM which have been co-treated with 200 μmol/L LPA (O832928, MREDA, Beijing, China) and different concentrations of CRO (1, 5, 10, 20 μmol/L) for 24 h. Then the cells were collected for later use. The concentrations of both LPA and CRO were selected according to those described previously but with slight modifications [31 (link),32 (link)].

Zhang F., Liu P., He Z., Zhang L., He X., Liu F, & Qi J. (2022). Crocin ameliorates atherosclerosis by promoting the reverse cholesterol transport and inhibiting the foam cell formation via regulating PPARγ/LXR-α. Cell Cycle, 21(2), 202-218.

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