Hct116

The human CRC cell lines (HCT116, SW480, CW‐2, RKO and DLD‐1) were purchased from Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (China). The human normal colon cell line (HCoEpiC) was purchased from BNCC (China). HCT116 cells were cultured in McCoy's 5A Media which had supplemented with 10% FBS, and the cells were humidified incubator with 5% CO2 at 37°C. HCoEpiC cells were cultured in DMEM, while SW480, CW‐2, RKO and DLD‐1 cells were cultured in RPMI 1640 with other condition same with HCT‐116.

Human CRC cell lines HCT116, HCT8 and LOVO were obtained from the Cell Resource Center, IBMS, CAMS/PUMC (Beijing, China). The cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (Gibco, USA) containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, USA) at 37°C in a humidified atmosphere comprising 5% CO₂.

The human cancer cell lines including AGS, HGC27, HeLa, Panc-1, HCT116, A549, and U2OS were purchased from the Cell Resource Center, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). MKN45 was purchased from the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China). Hucct1 were obtained from the Japanese Collection of Research Bioresources (JCRB) (Tokyo, Japan). The cell lines were originally authenticated in China Center for Type Culture Collection (CCTCC) by Short Tandem Repeat (STR) profiling and passaged less than 6 months in the lab. These cells are routinely maintained in the laboratory of our group.The acid cell culture medium (pH 6.5) was obtained by the addition of 1 M HCl solution as previously reported^{23 (link),24 (link)}. The pH was estimated by the use of a FE20 FiveEasy Plus pH Meter (METTLER TOLEDO Instruments (China), Shanghai, China).

The human cancer cell lines SW480, HCT116 and CaCO₂ were purchased from the Shanghai Institute of Cell Resource Center Life Science (Shanghai, P.R.C.). HCT116/DDP cells were purchased from Hunan Fenghui Biotechnology Co., Ltd (Changsha, P.R.C.). The cells were subjected to short tandem repeat analysis and validated as free from mycoplasma contamination. Cells were used within 6 months. All cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, CellMax), penicillin and streptomycin at 37°C in a humidified atmosphere with 5% CO₂ and were maintained in logarithmic growth phase for all experiments. HCT116/DDP cells were periodically cultured in 5 μg/ml DDP to maintain the DDP resistant phenotype and shifted into DDP-free medium for 1 week before use.