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Mang20

Manufactured by R&D Systems
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Sourced in United States

MANG20 is a high-performance liquid chromatography (HPLC) system designed for the analysis and purification of a wide range of biological samples. It features a sophisticated solvent delivery system, a versatile sample handling system, and advanced detection capabilities, making it a reliable and efficient tool for researchers and analysts working in the life sciences industry.

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Lab products found in correlation

Ab213909, by Abcam (3 mentions) Bio plex cell lysis kit, by Bio-Rad (3 mentions) Rlb00, by R&D Systems (3 mentions) Dang20, by R&D Systems (1 mentions) Mlb00c, by R&D Systems (1 mentions) Mta00b, by R&D Systems (1 mentions) Mmv00, by R&D Systems (1 mentions) Mbb00, by R&D Systems (1 mentions) Dy923, by R&D Systems (1 mentions)

11 protocols using mang20

1

Endothelial Dysfunction and Cytokine Profiling

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Plasma levels of Angiopoietin-2 (MANG20, R&D Systems, Minneapolis, MN, USA) and Endocan-1 (MBS039900, MyBioSource, San Diego, CA, USA) were measured as markers of endothelial dysfunction by ELISA according to the manufacturer’s protocol. Plasma, kidney, and lung IL-1β (RLB00, R&D Systems) and IL-18 (ab213909, Abcam, Boston, MA, USA) were quantified by ELISA according to the manufacturer’s protocol. Kidneys and lung lysates were prepared with the BioPlex Cell Lysis Kit (171304011, BioRad, Hercules, CA, USA) according to the manufacturer’s protocol.
Borges-Rodriguez M., Shields C.A., Travis O.K., Tramel R.W., Baik C.H., Giachelli C.A., Tardo G.A., Williams J.M, & Cornelius D.C. (2021). Platelet Inhibition Prevents NLRP3 Inflammasome Activation and Sepsis-Induced Kidney Injury. International Journal of Molecular Sciences, 22(19), 10330.
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2

ANGPT2 Protein Quantification in Cell Culture and Serum

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ANGPT2 protein levels in the cell culture supernatant and serum were determined using either mouse ANGPT2 (R&D, #MANG20) or human ANGPT2 (R&D, #DANG20) ELISA kits, according to the manufacturer’s protocol.
Pari A.A., Singhal M., Hübers C., Mogler C., Schieb B., Gampp A., Gengenbacher N., Reynolds L.E., Terhardt D., Géraud C., Utikal J., Thomas M., Goerdt S., Hodivala-Dilke K., Augustin H.G, & Felcht M. (2020). Tumor cell-derived Angiopoietin-2 promotes metastasis in melanoma. Cancer research, 80(12), 2586-2598.
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3

ANGPT2 Levels in Pituitary Adenomas

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ANGPT2 concentration in human plasma and supernatant of human cells was measured using a validated ELISA assay according to manufacturer’s instructions (DANGPT20; R&D Systems). Assays were run on the same day, on plates from the same batch, and the same control plasma samples (n = 4) were included in each plate for reproducibility. Using unpaired t‐test with Welch’s correction: mean ± SEM healthy controls (n = 69) = 0.89 ± 0.058; mean ± SEM PitNET patients (n = 69) = 1.78 ± 0.14; Difference between means = 0.92 ± 0.15; 95% confidence interval = 0.62 to 1.21; P value < 0.0001.
Angpt2 levels in the supernatant of GH3 cells and derivative clones, as well as of rat primary cells was assessed with a rat/mouse ELISA assay as above (MANG20; R&D Systems). To each measurement, the value of the serum‐free medium alone (blank) was subtracted. The values of the supernatants of shAngpt2‐infected cells were close to/lower than those of the blank, indicating no secretion of Angpt2.
Karabid N.M., Wiedemann T., Gulde S., Mohr H., Segaran R.C., Geppert J., Rohm M., Vitale G., Gaudenzi G., Dicitore A., Ankerst D.P., Chen Y., Braren R., Kaissis G., Schilling F., Schillmaier M., Eisenhofer G., Herzig S., Roncaroli F., Honegger J.B, & Pellegata N.S. (2022). Angpt2/Tie2 autostimulatory loop controls tumorigenesis. EMBO Molecular Medicine, 14(5), e14364.
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4

Measurement of Serum Biomarkers

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APOL1 (Proteintech, KE00047), Il-10 (R&D Systems, M1000B), Il-1 beta (R&D Systems, MLB00C), TNFα (R&D Systems, MTA00B) and Angpt2 (R&D Systems, MANG20) concentration in serum samples were measured by specific sandwich ELISA kits according to the manufacturer’s instructions.
Wu J., Ma Z., Raman A., Beckerman P., Dhillon P., Mukhi D., Palmer M., Chang Chen H., Robinson Cohen C., Dunn T., Reilly J., Meyer N., Shahaty M., Arany Z., Haskó G., Laudanski K., Hung A, & Susztak K. (2021). APOL1 risk variants in individuals of African genetic ancestry drive endothelial cell defects that exacerbate sepsis. Immunity, 54(11), 2632-2649.e6.
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5

Quantitative Tissue Biomarker Assay

Cited 1 time
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Harvested lung and kidney tissues were snap-frozen in liquid nitrogen
and homogenized in RIPA lysis buffer (27 (link)). Blood samples obtained via terminal cardiac punctures were
collected in heparin-coated plasma-separating tubes (BD Microtainer #365985) and
centrifuged for 10 minutes at 1,000 RCF (g) to isolate plasma.
Quantikine ELISA kits were used to measure murine Ang2 (R&D #MANG20),
VEGF (#MMV00), PDGF-BB (#MBB00), and erythropoietin (EPO; #MEP00) levels in
plasma or tissue homogenates.
Wu F.T., Man S., Xu P., Chow A., Paez-Ribes M., Lee C.R., Pirie-Shepherd S.R., Emmenegger U, & Kerbel R.S. (2016). Efficacy of Cotargeting Angiopoietin-2 and the VEGF Pathway in the Adjuvant Postsurgical Setting for Early Breast, Colorectal, and Renal Cancers. Cancer research, 76(23), 6988-7000.
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6

Maternal Plasma Angpt-1 and Angpt-2 ELISA

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Circulating mouse maternal plasma levels of Angpt-1 (1:20 dilution, R&D Systems, cat #DY923) and Angpt-2 (1:160 dilution, R&D Systems, cat#MANG20) were measured by enzyme-linked immunosorbent assay (ELISA) from samples stored at -80°C. Samples were processed according to manufacturer's protocols. Laboratory personnel were blinded to experimental groups and outcomes.
Tran V., Weckman A.M., Crowley V.M., Cahill L.S., Zhong K., Cabrera A., Elphinstone R.E., Pearce V., Madanitsa M., Kalilani-Phiri L., Mwapasa V., Khairallah C., Conroy A.L., ter Kuile F.O., Sled J.G, & Kain K.C. (2021). The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy. EBioMedicine, 73, 103683.
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7

Blocking Proliferation in 2D and 3D Cell Cultures

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RAW264.7 cells were seeded at the same concentration (3 × 105 cells/mL) in both 3D and 2D culture systems. To prevent cell proliferation, the cells were treated with γ-RAY at 3 Gy to block cell proliferation. After 24 h, the culture medium from both systems was collected and the cells were counted to show that cell proliferation had been blocked successfully. The supernatant medium was collected for ELISA with a mouse VEGF ELISA kit (EK283/2-01, Multiscience (LIANKE) Biotech, Shanghai, China) and a mouse angiopoietin-2 immunoassay (MANG20, R&D Systems Inc., USA) according to the manufacturer’s instructions. The plates were read using a microplate reader at 450 nm, and the wavelength was corrected to 570 nm. All tests were performed in duplicate.
Shi H., Li D., Shi Q., Han Z., Tan Y., Mu X., Qin M, & Li Z. (2021). Three-Dimensional Culture Decreases the Angiogenic Ability of Mouse Macrophages. Frontiers in Immunology, 12, 795066.
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8

Endothelial Permeability and Inflammatory Markers

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Plasma levels of Angiopoietin‐2 (MANG20, R&D Systems) and Endocan‐1 (MBS039900, MyBioSource, San Diego, CA) were measured as markers of endothelial permeability by ELISA according to the manufacturer's protocol. Plasma, kidney, and lung IL‐1β (RLB00, R&D Systems) and IL‐18 (ab213909, Abcam, Boston, MA) were quantified by ELISA according to the manufacturer's protocol. Levels of IL‐1β in collected platelet media were also quantified by ELISA according to the manufacturer's protocol. Kidney and lung lysates were prepared with the BioPlex Cell Lysis Kit (171304011, BioRad, Hercules, CA) according to the manufacturer's protocol. Tissue cytokine levels were normalized to protein concentration using a protein assay with BSA standards (Pierce, Rockford, IL).
Cornelius D.C., Baik C.H., Travis O.K., White D.L., Young C.M., Austin Pierce W., Shields C.A., Poudel B, & Williams J.M. (2019). NLRP3 inflammasome activation in platelets in response to sepsis. Physiological Reports, 7(9), e14073.
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9

Angiopoietin ELISA Assay in Mice

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Mice were treated according to the above-described schedule. Blood was drawn 2 or 4 hours post CS challenge via cardiac puncture. Blood was allowed to clot at room temperature for 15 minutes at room temperature prior to centrifugation to isolate serum. Mouse Ang1 (Sapphire Biosciences: #LS-F2956) and Ang2 (R&D Systems: #MANG20) ELISAs were performed according to manufacturer instructions.
Fidanza M., Hibbert J., Acton E., Harbeson D., Schoeman E., Skut P., Woodman T., Eynaud A., Hartnell L., Brook B., Cai B., Lo M., Falsafi R., Hancock R.E., Chiume-Kayuni M., Lufesi N., Popescu C.R., Lavoie P.M., Strunk T., Currie A.J., Kollmann T.R., Amenyogbe N, & Lee A.H. (2024). Angiogenesis-associated pathways play critical roles in neonatal sepsis outcomes. Scientific Reports, 14, 11444.
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10

Plasma and Tissue Biomarkers of Endothelial Dysfunction

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Plasma levels of Angiopoietin-2 (MANG20, R&D Systems) and Endocan-1 (MBS039900, MyBioSource, San Diego, CA) were measured as markers of endothelial permeability by ELISA according to the manufacturer’s protocol. Plasma, kidney, and lung IL-1β (RLB00, R&D Systems) and IL-18 (ab213909, Abcam, Cambridge, MA) were quantified by ELISA according to the manufacturer’s protocol. Kidneys and lung lysates were prepared with the BioPlex Cell Lysis Kit (171304011, BioRad, Hercules, CA) according to the manufacturer’s protocol. Plasma levels of IL-6, IL-17, and IL-10 were quantified by luminex multi-bead technology (Bio-Rad). Plasma levels of Platelet Factor 4 (a marker of platelet activation) were measured by ELISA (MCX400, RnD Systems). Protein was quantified by BCA analysis in kidney and lung lysates and culture media. IL-1β was quantified in platelet culture media via ELISA. Data from kidney and lung lysates, and culture media were normalized to protein.
Cornelius D.C., Travis O.K., Tramel R.W., Borges-Rodriguez M., Baik C.H., Greer M., Giachelli C.A., Tardo G.A, & Williams J.M. (2020). NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture. PLoS ONE, 15(6), e0234039.
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