Ck40 inverted microscope

MDA231, Hs578T, and HCC1806 TNBC cells were seeded in 6-well plates and cultured for 24 h. Then, the TNBC cells were maintained in culture medium without FBS for 16-24 h. The cell monolayer was scratched with a 200-μL pipette tip to create a wound, which was washed twice with PBS to remove the suspended cells. TNBC cells were maintained with or without 10 μM LY2109761 for 24 h in serum-containing medium. The cells migrating from the leading edge were photographed at 0 and 24 h using a CK40 inverted microscope (Olympus, Tokyo, Japan). Cell to cell distance was analyzed average of pixel number on three sites using Axiovision software (Carlzeiss, Thornwood, NY).

The morphologies of myotubes were observed using a CK40 inverted microscope (Olympus, Japan) equipped with a T500 camera (magnification, ×200; eXcope, Korea). The random areas of each group were captured and the diameters were measured using the Image J software (National Institutes of Health, USA).

Morphological features were studied under an Olympus CK40 inverted microscope at 20× magnification. Images were captured using VisiCam software (VWR, USA).

Morphological features were studied under an Olympus CK40 inverted microscope at 40 x magnification. Images were captured using VisiCam® software (VWR, USA).

After liver samples were collected, one fresh section of liver tissue from each rat was used to prepare frozen sections. Each section was incubated in liquid nitrogen for 4–10 s prior to staining with Oil Red O (Sigma-Aldrich, St. Louis, MO, USA). The mean optical density (MOD) was calculated to quantify the intracellular lipid deposition, using the image analysis software Image Pro-plus 6.0 (Media Cybernetics, USA). One more fresh section of liver tissue from each rat was fixed by immersion in 10% buffered formalin for paraffin wax embedding prior to staining with H&E. The histological examination was performed by a pathologist blinded to the rats' data. The samples were observed using an Olympus CK40 inverted microscope (Olympus, Tokyo, Japan).

A minimum of 200 non-naupliar organisms were subsampled from aliquots of each zooplankton sample using a Stempel pipette, which were examined using a Leica MZ6 stereomicroscope (Leica Microsystems) at 40X magnification. Specimens were identified to the lowest possible taxonomic rank using Thorp and Covich [35 ], with most rotifers and microcrustaceans identified to the genus or species level. We converted counts of individual taxa to density (individuals m^–3) by multiplying each count by the ratio of sample volume to subsample volume, and then dividing by the total volume of water filtered. Two replicate samples were processed for each sampling date.
Microplankton samples (i.e. planktonic protists, eukaryotic algae, cyanobacteria) were taxonomically processed via 1–10 ml subsamples of the Lugol’s preserved water samples. Subsamples were settled overnight in Utermohl chambers, and the chambers examined using an Olympus CK-40 inverted microscope at 200X (numerical aperture = 0.75). All individuals were identified to genus (and species when possible) using Wehr et al. [58 ] and sized using an ocular micrometer in order to calculate biovolume and carbon biomass based on geometric shape [59 (link), 60 (link)].