Dexamethasone and sialic acid were purchased from Aladdin Bio-chem Technology Co. Limited (Shanghai, China). HOOC-PEG-COOH (Mw = 2.0 kDa) was purchased from Sigmae-Aldrich Inc., USA. Fluorescein isothiocyanate (FITC), indocyanine green (ICG) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies, including Anti-Bax, Anti-Bcl-2, Anti-Caspase-3, Anti-CD62E(E-selectin), Anti-Beclin 1, Anti-Atg5 and Anti-Atg5/12 antibodies were purchased from Abcom. A TUNEL assay kit was obtained from Roche (Nutley, NJ, USA). All other solvents were of analytical or chromatographic grade.
Human umbilical vein endothelial cells (HUVECs) were purchased from the Thermo Fisher. HUVECs were maintained in RPMI-1640 containing 10% fetal bovine serum (Gibco) as well as 1% penicillin and streptomycin (Sigma) and were cultured at 37°C in a humidified atmosphere of 5% CO2.
ICR mice (body weight: 18-20 g) were purposed from the Zhejiang Medical Animal Centre. All animal experiments were carried out in accordance with the National Institutes of Health (NIH, USA) guidelines for the care and use of laboratory animals in research. The surgical procedures and experiment protocols were approved by the Committee for Animal Experiments of Zhejiang University.
Human umbilical vein endothelial cells (HUVECs) were purchased from the Thermo Fisher. HUVECs were maintained in RPMI-1640 containing 10% fetal bovine serum (Gibco) as well as 1% penicillin and streptomycin (Sigma) and were cultured at 37°C in a humidified atmosphere of 5% CO2.
ICR mice (body weight: 18-20 g) were purposed from the Zhejiang Medical Animal Centre. All animal experiments were carried out in accordance with the National Institutes of Health (NIH, USA) guidelines for the care and use of laboratory animals in research. The surgical procedures and experiment protocols were approved by the Committee for Animal Experiments of Zhejiang University.