Five EPCR shRNA clones and one control shRNA lentiviral particles were obtained from Sigma‐Aldrich (St Louis, Missouri, USA) (supplementary materials and methods). HCT116 cells or HT29 cells were grown to 70–80% confluence in Dulbecco's Modified Eagle Medium (DMEM) with Fetal Calf Serum (FCS). shRNA lentiviral particles were added at multiplicity of infection (MOI) of 5, 1.5 and 0.5. shRNA transductants were selected for Puromycin (Sigma‐Aldrich, St Louis, Missouri, USA) resistance. EPCR‐overexpressing HT29 cells were a kind gift from Julie Déchanet‐Merville (University of Bordeaux, France).
Control shrna lentiviral particles
Manufactured by Merck Group
Sourced in United States
Control shRNA lentiviral particles are a type of laboratory reagent used in gene silencing experiments. They contain a non-targeting short hairpin RNA (shRNA) sequence packaged into lentiviral particles. These control particles can be used as a negative control to evaluate the effects of gene knockdown when compared to cells transduced with test shRNA constructs.
Automatically generated - may contain errors
2 protocols using control shrna lentiviral particles
1
Lentiviral EPCR Knockdown in Colon Cancer Cells
2
Reducing TRiC Levels via CCT2 shRNA
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To reduce TRiC levels within cells, MISSION shRNA lentiviral transduction particles targeting CCT2 sequence (SHCLNV-NM-006431) and control shRNA lentiviral particles were obtained from Sigma-Aldrich. HepG2 and HS-578T cells were transduced with CCT2 shRNA or control shRNA lentiviral particles at an MOI of 5 and grown for 48 h. Stable TRiC knockdown cells were established by replating the cells in selection media containing puromycin at 3 µg/ml.
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