HPLC grade isopropyl alcohol, acetonitrile, methanol, and water, laboratory grade formic acid (FA), and ammonium bicarbonate (AmBic) were purchased from Fisher Scientific (Waltham, MA). Pierce™ Peptide Retention Time Calibration Mixture (PRTC) was from ThermoFisher Scientific (San Jose, CA). Human apolipoprotein A1 (ApoA1) uniformly 15N-labeled and enriched (isotopic enrichment >98% 15N by LC/MS, purity > 90% by SDS-PAGE) was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). Trifluoroethanol (TFE), dithiothreitol (DTT), iodoacetamide (IAA), and ammonium hydroxide were purchased from Sigma Aldrich (St. Louis, MO). Hydrochloric acid was from JT Baker (Phillipsburg, NJ). Worthington trypsin was used for proteolytic digestion (Lakewood, NJ).
Pierce peptide retention time calibration mixture prtc
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Pierce™ Peptide Retention Time Calibration Mixture (PRTC) is a laboratory product designed to calibrate the retention time of peptides in liquid chromatography-mass spectrometry (LC-MS) analyses. The mixture contains a set of known peptides that can be used to establish and monitor the retention time behavior of the LC-MS system.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium bicarbonate ambic, by Thermo Fisher Scientific (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Avantor (1 mentions)
Formic acid, by Merck Group (1 mentions)
Sep pak vac c18 cartridge, by Waters Corporation (1 mentions)
Sequencing grade trypsin, by Promega (1 mentions)
Benchmark protein ladder, by Thermo Fisher Scientific (1 mentions)
2 protocols using pierce peptide retention time calibration mixture prtc
1
Quantitative Proteomic Analysis of ApoA1
2
Quantitative Proteomics Protocol
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Unless otherwise specified,
all chemicals and reagents were obtained from Sigma-Aldrich (Steinheim,
Germany). Formic acid (FA) was from Merck (Darmstadt, Germany). Acetonitrile
(ACN) was purchased from Biosolve (Valkenswaard, The Netherlands).
Sequencing grade trypsin was obtained from Promega (Madison, WI).
The 12% Criterion XT Bis-Tris Protein Gel and SDS-PAGE sample buffer
were purchased from Bio-Rad (Hercules, CA, US). Pierce peptide retention
time calibration mixture (PRTC), BenchMark ProteinLadder, and GelCold
Blue Stain Reagent were from Thermo Fisher Scientific (Bremen, Germany).
The Sep-Pak C18 vac cartridge was purchased from Waters (Massachusetts,
USA). Milli-Q was produced by an in-house system (Millipore, Billerica,
MA). The stable isotope-labeled peptides (crude SpikeTides_L) were
synthesized by JPT Peptide Technologies (Berlin, Germany).
all chemicals and reagents were obtained from Sigma-Aldrich (Steinheim,
Germany). Formic acid (FA) was from Merck (Darmstadt, Germany). Acetonitrile
(ACN) was purchased from Biosolve (Valkenswaard, The Netherlands).
Sequencing grade trypsin was obtained from Promega (Madison, WI).
The 12% Criterion XT Bis-Tris Protein Gel and SDS-PAGE sample buffer
were purchased from Bio-Rad (Hercules, CA, US). Pierce peptide retention
time calibration mixture (PRTC), BenchMark ProteinLadder, and GelCold
Blue Stain Reagent were from Thermo Fisher Scientific (Bremen, Germany).
The Sep-Pak C18 vac cartridge was purchased from Waters (Massachusetts,
USA). Milli-Q was produced by an in-house system (Millipore, Billerica,
MA). The stable isotope-labeled peptides (crude SpikeTides_L) were
synthesized by JPT Peptide Technologies (Berlin, Germany).
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