Cd15 fitc

Manufactured by Miltenyi Biotec

Sourced in United States

CD15-FITC is a fluorescent-labeled monoclonal antibody that binds to the CD15 antigen. CD15 is a carbohydrate antigen expressed on the surface of granulocytes, monocytes, and a subset of lymphocytes. The FITC (fluorescein isothiocyanate) label allows for the detection and identification of CD15-positive cells using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Zombie dye yellow staining, by BioLegend (2 mentions) Cd3 apc, by BioLegend (2 mentions) Cd45 vioblue450, by Cytek Biosciences (2 mentions) Cd3 viogreen, by Miltenyi Biotec (2 mentions) Cd11c percp cy5, by BioLegend (2 mentions) Cd11b pe, by Cytek Biosciences (2 mentions) Cd66b apc, by Miltenyi Biotec (2 mentions) Cd1c pe dazzle, by BioLegend (2 mentions) Hla dr bv570, by BioLegend (2 mentions) Cd14 e780, by Thermo Fisher Scientific (2 mentions) Macsquant 10, by Miltenyi Biotec (2 mentions) Ab59776, by Abcam (1 mentions) Cd34 pe, by Miltenyi Biotec (1 mentions) Cd31 pe, by BD (1 mentions) Cd31 fitc, by BD (1 mentions) Oct4 c 10, by Santa Cruz Biotechnology (1 mentions) Mouse anti human cd45 fitc, by Miltenyi Biotec (1 mentions) Lamp2, by Abcam (1 mentions) Anti mouse igg cy3, by Jackson ImmunoResearch (1 mentions) Cd14 apc, by Miltenyi Biotec (1 mentions)

5 protocols using cd15 fitc

Comprehensive Antibody Panel for Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used for flow cytometry, cell sorting and immunostainings respectively: mouse anti-human CD34-APC (Miltenyi and BD biosciences), CD34-PE (Miltenyi), mouse anti-human CD45-FITC (Miltenyi), CD45-APC (BD biosciences), CD45-VB (Miltenyi), mouse anti-human HLA ABC-VB (BD biosciences), CD31-FITC (BD biosciences), CD31-PE (BD biosciences), CD14-APC (Miltenyi), CD15-FITC (Miltenyi), mouse anti-human CD-133-APC (Miltenyi), mouse anti-human CD38-PE (BD biosciences), mouse anti-human CD90-FITC (BD biosciences), HLA-PE (BD biosciences), mouse APC isotype control (BD biosciences), mouse FITC isotype control (BD biosciences), mouse PE isotype control and mouse Vioblue isotype control (BD biosciences), OCT4 (C-10, SantaCruz, sc-5279, 1:100), LAMP-2 (1:50, Abcam), α-tubulin mouse IgG (1:500; Sigma), Anti mouse IgG-Cy3 (1:200; Jackson), Anti rabbit-IgG- Dylight 649 (1:200; Jackson), Alexa fluor 488-, 594- or 647-conjugated anti-rabbit or anti-mouse or anti-goat secondary antibodies (1/500, Jackson Immunoresearch), DAPI (5 mg ml⁻¹) (1:2000; Invitrogen).
For ChIP assay experiment, the following antibodies were used: anti-rabbit-IgGs (sc-2027 Santa Cruz Biotechnology), and rabbit anti-SOX2 (ab59776; Abcam). A total of 5 μg of each antibody was used for ChIP experiments.

Pulecio J., Nivet E., Sancho-Martinez I., Vitaloni M., Guenechea G., Xia Y., Kurian L., Dubova I., Bueren J., Laricchia-Robbio L, & Belmonte J.C. (2014). Conversion of human fibroblasts into monocyte-like progenitor cells. Stem cells (Dayton, Ohio), 32(11), 2923-2938.

+ Open protocol

+ Expand

Multi-Parametric Flow Cytometry of Immune Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue450, CD11b-PE (Tonbo, San Diego, CA), CD3-VioGreen, CD15-FITC, CD66b-APC (Miltenyi biotec), CD11c-PerCp-Cy5.5, CD1c-PE-dazzle, HLA-DR-BV570, CD3-APC (Biolegend), CD14-e780, (eBiosciences, San Diego, CA). Dead cells were excluded with 7AAD (Southern Biotech) or zombie dye yellow staining (Biolegend). Analysis was performed on 8-color MACSQuant 10 (Miltenyi biotech) or Gallios (Beckman Coulter, Indianapolis, IN) flow cytometers and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers is shown as percentage of positive cells. Fluorescence minus one (FMO) strategy was used to establish appropriate gates. Cells were gated as described before^{26 (link)}.

Barr F.D., Ochsenbauer C., Wira C.R, & Rodriguez-Garcia M. (2018). Neutrophil extracellular traps prevent HIV infection in the female genital tract. Mucosal immunology, 11(5), 1420-1428.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Analysis of Neutrophil Surface Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neutrophils were fixed with 4% PFA for 30 min at 4 °C, washed and stained for 20 min in the dark with the following anti-human antibodies: CD45-APC-Cy7 (clone 2D1; Biolegend, San Diego, CA, USA), CD54-BV421 (clone HA58; Biolegend), CD62L-BV711 (clone SK11; Biolegend), CXCR4-BV-785 (clone 12G5; Biolegend), CD66b-APC (clone REA306; Miltenyi Biotec), CD15-FITC (Miltenyi Biotec) and CD16-PE (clone 3G8; BD Biosciences, Franklin Lakes, NJ, USA). A live/dead fixable blue dead cell stain kit (Thermo Scientific; Waltham, MA, USA) was used to assess cell death in cultures before fixation. Fluorescence Minus One (FMO) controls were used to identify and gate positive populations (Figure 1 and Figure S1b). Analysis was performed on an LSRII flow cytometer (BD; Ashland, Wilmington, DE, USA) or Aurora cytometer (Cytek Biosciences; Fremont, CA, USA) and assessed using FlowJo software (BD) or OMIQ (www.omiq.ai (accessed on 13 July 2021)). The expression of surface markers was measured by the percentage of positive cells.

Carrillo-Salinas F.J., Parthasarathy S., Moreno de Lara L., Borchers A., Ochsenbauer C., Panda A, & Rodriguez-Garcia M. (2022). Short-Chain Fatty Acids Impair Neutrophil Antiviral Function in an Age-Dependent Manner. Cells, 11(16), 2515.

+ Open protocol

+ Expand

Multi-Parametric Flow Cytometry of Immune Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Barr F.D., Ochsenbauer C., Wira C.R, & Rodriguez-Garcia M. (2018). Neutrophil extracellular traps prevent HIV infection in the female genital tract. Mucosal immunology, 11(5), 1420-1428.

+ Open protocol

+ Expand

Isolation of Monocytes from COVID-19 PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs were obtained from peripheral blood by Ficoll gradient using Lymphocyte Isolation Solution (Rafer, Zaragoza, Spain) from 48 of the severe COVID-19 patients and 11 HDs. Once PBMCs were isolated, all samples were stored at − 150 °C in 10% DMSO in fetal bovine serum (FBS) until monocyte purification. The monocyte population was isolated by flow cytometry (FacsAria Fusion, BD, Beckton Dickinson, San Jose, CA, USA). PBMCs were stained with CD14-APC-Vio770 (Miltenyi Biotec) and CD15-FITC (Miltenyi Biotec) in staining buffer (MACS) for 20 min. A gating strategy was employed to eliminate cell debris, doublets, and DAPI + cells. CD14 and CD15 antibodies were used to isolate CD14 + CD15 − . Purified cells were pelleted and stored at − 80 °C.
After monocyte isolation, DNA was isolated using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) following the manufacturer’s instructions.

Godoy-Tena G., Barmada A., Morante-Palacios O., de la Calle-Fabregat C., Martins-Ferreira R., Ferreté-Bonastre A.G., Ciudad L., Ruiz-Sanmartín A., Martínez-Gallo M., Ferrer R., Ruiz-Rodriguez J.C., Rodríguez-Ubreva J., Vento-Tormo R, & Ballestar E. (2022). Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines. Genome Medicine, 14, 134.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!