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Heat inactivated fcs

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Heat-inactivated Fetal Calf Serum (FCS) is a cell culture supplement derived from the blood of bovine fetuses. The serum is inactivated through heat treatment, which denatures proteins and inactivates potential contaminants. This process ensures the serum is suitable for use in a variety of cell culture applications.

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Lab products found in correlation

Sodium pyruvate, by GE Healthcare (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 media, by GE Healthcare (2 mentions) Non essential amino acids (neaa), by GE Healthcare (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Dmem media, by Thermo Fisher Scientific (2 mentions)

2 protocols using heat inactivated fcs

1

Culturing and Implanting Tumor Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
B16-F10, TC-1, and B16.OVA were cultured in RPMI-1640 media (GE Life
Sciences), and MC38 was cultured in DMEM media (Gibco), each supplemented with
10% v/v heat-inactivated FCS (Atlanta Biologicals), 100 U/mL penicillin, 100
μg/mL streptomycin (Gibco), 1× non-essential amino acids (GE Life
Sciences), and 1 mM sodium pyruvate (GE Life Sciences). B16.OVA media was
supplemented with 0.5 mg/mL G418. For each tumor cell implantation, a frozen
aliquot was thawed, passaged once, and harvested using trypsin EDTA (Gibco),
quenched with HI-FCS, washed in PBS, and implanted subcutaneously in sterile
PBS. Tumors were measured using digital calipers twice per week, and tumor
volume was estimated using the formula [tumor volume = short × short
× long / 2]. Animals were euthanized when tumors reached size criteria
(1000 mm3 or 2000 mm3).
Lynn G.M., Sedlik C., Baharom F., Zhu Y., Ramirez-Valdez R.A., Coble V.L., Tobin K., Nichols S.R., Itzkowitz Y., Zaidi N., Gammon J.M., Blobel N.J., Denizeau J., de la Rochere P., Francica B.J., Decker B., Maciejewski M., Cheung J., Yamane H., Smelkinson M.G., Francica J.R., Laga R., Bernstock J.D., Seymour L.W., Drake C.G., Jewell C.M., Lantz O., Piaggio E., Ishizuka A.S, & Seder R.A. (2020). Peptide-TLR-7/8a conjugate vaccines chemically programmed for nanoparticle self-assembly enhance CD8 T cell immunity to tumor antigens. Nature biotechnology, 38(3), 320-332.
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2

Culturing and Implanting Tumor Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
B16-F10, TC-1, and B16.OVA were cultured in RPMI-1640 media (GE Life
Sciences), and MC38 was cultured in DMEM media (Gibco), each supplemented with
10% v/v heat-inactivated FCS (Atlanta Biologicals), 100 U/mL penicillin, 100
μg/mL streptomycin (Gibco), 1× non-essential amino acids (GE Life
Sciences), and 1 mM sodium pyruvate (GE Life Sciences). B16.OVA media was
supplemented with 0.5 mg/mL G418. For each tumor cell implantation, a frozen
aliquot was thawed, passaged once, and harvested using trypsin EDTA (Gibco),
quenched with HI-FCS, washed in PBS, and implanted subcutaneously in sterile
PBS. Tumors were measured using digital calipers twice per week, and tumor
volume was estimated using the formula [tumor volume = short × short
× long / 2]. Animals were euthanized when tumors reached size criteria
(1000 mm3 or 2000 mm3).
Lynn G.M., Sedlik C., Baharom F., Zhu Y., Ramirez-Valdez R.A., Coble V.L., Tobin K., Nichols S.R., Itzkowitz Y., Zaidi N., Gammon J.M., Blobel N.J., Denizeau J., de la Rochere P., Francica B.J., Decker B., Maciejewski M., Cheung J., Yamane H., Smelkinson M.G., Francica J.R., Laga R., Bernstock J.D., Seymour L.W., Drake C.G., Jewell C.M., Lantz O., Piaggio E., Ishizuka A.S, & Seder R.A. (2020). Peptide-TLR-7/8a conjugate vaccines chemically programmed for nanoparticle self-assembly enhance CD8 T cell immunity to tumor antigens. Nature biotechnology, 38(3), 320-332.
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