Ms400 18 38 mhz transducer

Manufactured by Fujifilm

Sourced in Canada

The MS400 18-38 MHz transducer is a lab equipment product manufactured by Fujifilm. It is a high-frequency ultrasound transducer designed for research and laboratory applications. The transducer operates within the frequency range of 18 to 38 MHz, enabling high-resolution imaging and analysis.

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Lab products found in correlation

Vevolab software, by Fujifilm (3 mentions) Vevo 2100 system, by Fujifilm (2 mentions) Vevo 2100 imaging system, by Fujifilm (2 mentions) Vevo 2100, by Fujifilm (1 mentions) Avertin, by Merck Group (1 mentions) Visualsonics ultrasound system, by Fujifilm (1 mentions)

Spelling variants of this product

18–38 MHz transducer (2 citations)

6 protocols using ms400 18 38 mhz transducer

Echocardiography for Cardiac Function Assessment

Cited 3 times

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Echocardiography was performed using a Vevo 2100 equipped with a MS400 18-38 MHz transducer (Visualsonics) as in [16 (link),20 (link),29 (link)]. Mice were anaesthetized in an induction chamber with isoflurane (5% flow rate) with 1 l/min O₂ and transferred to the heated Vevo Imaging Station. Anaesthesia was maintained with 1.5% isoflurane delivered via a nose cone. Left ventricular cardiac function and structure was assessed from short axis M-mode images with the axis placed at the mid-level of the left ventricle at the level of the papillary muscles. Left ventricular mass and chamber volumes were measured from B-mode long axis images using VevoStrain software for speckle tracking. Baseline scans were taken prior to experimentation (7 to 3 days). Further scans were taken at intervals following minipump implantation. Imaging was completed within 20 min. Data analysis (Vevo LAB) was performed by an independent assessor blinded to intervention. Data were gathered from two scans taken from each time point, taking mean values across four cardiac cycles for each M-mode scan or two cardiac cycles for long axis B-mode images. Mice were recovered singly and transferred to the home cage once fully recovered.

Alharbi H.O., Hardyman M.A., Cull J.J., Markou T., Cooper S.T., Glennon P.E., Fuller S.J., Sugden P.H, & Clerk A. (2022). Cardiomyocyte BRAF is a key signalling intermediate in cardiac hypertrophy in mice. Clinical Science (London, England : 1979), 136(22), 1661-1681.

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Neonatal Rat Echocardiographic Analysis

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Echocardiographic analysis was performed on the neonatal rats using a Vevo2100 system equipped with a MS400 18–38 mHz transducer (VisualSonics), as previously described(9 (link)).

Blakeslee W.W., Demos-Davies K.M., Lemon D.D., Lutter K.M., Cavasin M.A., Payne S., Nunley K., Long C.S., McKinsey T.A, & Miyamoto S.D. (2017). Histone Deacetylase Adaptation in Single Ventricle Heart Disease and a Young Animal Model of Right Ventricular Hypertrophy. Pediatric research, 82(4), 642-649.

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Neonatal Rat Echocardiographic Analysis

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Echocardiographic analysis was performed on the neonatal rats using a Vevo2100 system equipped with a MS400 18–38 mHz transducer (VisualSonics), as previously described(9 (link)).

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Cardiac Ultrasound Evaluation in Mice

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Ultrasound examination of the left ventricle (LV) was performed at 4, 8, and/or 16 weeks post-RT using a Fujifilm VisualSonics Ultrasound System (VisualSonics Inc, Toronto, ON, Canada) and using an MS400 (18–38 MHZ) transducer. Mice were lightly anesthetized with an intraperitoneal injection of 0.005 ml/g of 2% Avertin (2,2,2-Tribromoethanol, Sigma-Aldrich, St. Louis, MO). Hair was removed from the anterior chest using chemical hair remover (Nair), and the animals were placed on a warming pad in a left lateral decubitus position to maintain normothermia (37°C), monitored by a rectal thermometer. Ultrasound gel was applied to the chest. Care was taken to maintain adequate contact while avoiding excessive pressure on the chest. Two-dimensional long-axis, short-axis M-Mode images were obtained to evaluate LV systolic function. Trans mitral inflow pattern and tissue Doppler were obtained in modified 4 chamber apical view to evaluate LV diastolic function. After completion of the imaging studies, mice were allowed to recover from anesthesia and returned to their cages. M Mode Images were analyzed for LV structure and function related parameters and Pulse wave and tissue Doppler images were analyzed for diastolic function related parameters using Vevo Lab software (Visual Sonics Inc, Toronto, ON, Canada). Analyses were performed blinded to treatment group.

Dreyfuss A.D., Goia D., Shoniyozov K., Shewale S.V., Velalopoulou A., Mazzoni S., Avgousti H., Metzler S.D., Bravo P.E., Feigenberg S.J., Ky B., Verginadis I.I, & Koumenis C. (2021). A novel mouse model of radiation-induced cardiac injury reveals biological and radiological biomarkers of cardiac dysfunction with potential clinical relevance. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(8), 2266-2276.

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Echocardiography in Mice for Cardiac Assessment

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Echocardiography was performed on anaesthetised mice using a Vevo 2100 imaging system equipped with a MS400 18–38 MHz transducer (Visualsonics). Mice were anaesthetised in an induction chamber with isoflurane (5% flow rate) with 1 l/min O₂ then transferred to the heated Vevo Imaging Station. Anaesthesia was maintained with 1.5% isoflurane delivered via a nose cone. Baseline scans were taken prior to experimentation (−7 to −3 days). Further scans were taken at intervals following tamoxifen treatment or minipump implantation. Imaging was completed within 20 min. Mice were recovered singly and transferred to the home cage once fully recovered. Left ventricular cardiac dimensions were assessed from short axis M-mode images with the axis placed at the mid-level of the left ventricle at the level of the papillary muscles. Data analysis was performed using VevoLAB software (Visualsonics) and data were gathered from two M-mode scans at each time point, taking mean values across four cardiac cycles. Cardiac function and global longitudinal strain were measured from B-mode long axis images using VevoStrain software for speckle tracking. Mice were culled by CO₂ inhalation followed by cervical dislocation. Hearts were excised quickly, washed in PBS, dried and snap-frozen in liquid N₂ or fixed for histology.

Clerk A., Meijles D.N., Hardyman M.A., Fuller S.J., Chothani S.P., Cull J.J., Cooper S.T., Alharbi H.O., Vanezis K., Felkin L.E., Markou T., Leonard S.J., Shaw S.W., Rackham O.J., Cook S.A., Glennon P.E., Sheppard M.N., Sembrat J.C., Rojas M., McTiernan C.F., Barton P.J, & Sugden P.H. (2022). Cardiomyocyte BRAF and type 1 RAF inhibitors promote cardiomyocyte and cardiac hypertrophy in mice in vivo. Biochemical Journal, 479(3), 401-424.

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Echocardiography in Anaesthetized Mice

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Echocardiography was performed on anaesthetised mice using a Vevo 2100 imaging system equipped with a MS400 18-38 MHz transducer (Visualsonics). Mice were anaesthetised in an induction chamber with isoflurane (5% flow rate) with 1 l/min O 2 then transferred to the heated Vevo Imaging Station. Anaesthesia was maintained with 1.5% isoflurane delivered via a nose cone. Baseline scans were taken prior to experimentation (-7 to -3 days). Further scans were taken at intervals following tamoxifen treatment or minipump implantation. Imaging was completed within 20 min. Mice were recovered singly and transferred to the home cage once fully recovered. Left ventricular cardiac dimensions were assessed from short axis M-mode images with the axis placed at the mid-level of the left ventricle at the level of the papillary muscles. Data analysis was performed using VevoLAB software (Visualsonics) and data were gathered from two M-mode scans at each time point, taking mean values across 4 cardiac cycles. Cardiac function and global longitudinal strain were measured from B-mode long axis images using VevoStrain software for speckle tracking. Mice were culled by CO 2 inhalation followed by cervical dislocation.
Hearts were excised quickly, washed in PBS, dried and snap-frozen in liquid N 2 or fixed for histology.

Clerk A., Meijles D.N., Hardyman M.A., Fuller S.J., Chothani S., Cull J.J., Cooper S.T., Alharbi H.O., Vanezis K., Felkin L.E., Markou T., Leonard S.J., Shaw S., Rackham O.J.L., Cook S.A., Glennon P.E., Sheppard M.N., Sembrat J., Rojas M., McTiernan C.F., Barton P.J., & Sugden P.H. (2021). Cardiomyocyte BRAF and type 1 RAF inhibitors promote cardiomyocyte and cardiac hypertrophy in mice in vivo.

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