Sc 376594

Manufactured by Abcam

Sourced in United States

Sc-376594 is a primary antibody product manufactured by Abcam. It is designed for use in various laboratory applications, such as Western Blotting and Immunohistochemistry. The product details and intended use information are available on the Abcam website.

Automatically generated - may contain errors

Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Ab109373, by Proteintech (2 mentions) Ab85762, by Merck Group (2 mentions) Pierce reversible protein stain kit for pvdf membranes, by Thermo Fisher Scientific (2 mentions) T5168 100ul, by Merck Group (2 mentions) Sc 2357, by Santa Cruz Biotechnology (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Chemidoc imaging system, by Bio-Rad (2 mentions) Ab27242, by Abcam (1 mentions) Ab105298, by Abcam (1 mentions) Epon araldite mixture, by Electron Microscopy Sciences (1 mentions) Superblock blocking buffer, by Thermo Fisher Scientific (1 mentions)

3 protocols using sc 376594

Western Blot Analysis of Heart Proteins

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HCFs or mouse heart tissue were lysed in RIPA buffer. Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher, PI23227) according to manufacturer’s instructions. Western blot was performed as previously described ⁵⁵. Total protein was examined using Pierce Reversible Protein Stain Kit for PVDF membranes (Thermo Fisher, PI24585) and imaged using Bio-Rad ChemiDoc Imaging System. Target protein was detected using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher, 34577). Target band intensities were quantified using ImageJ software (National Institutes of Health). Primary Antibodies used include: FTH1 (1:1000, Santa Cruz Biotechnology, sc-376594), FTL (1:1000, Abcam, ab109373), PTGS2 (1:1000, Proteintech, 12375-1-AP), TSP1 (1:1000, Abcam, ab85762), and α-TUBULIN (1:1000, Sigma, T5168-100UL). Secondary antibodies include goat-anti-mouse (1:2500, Thermo Invitrogen, 32430) or goat-anti-rabbit (1:2500, Santa Cruz Biotechnology, sc-2357) horseradish peroxidase (HRP)-conjugated antibodies.

Mohr M.E., Li S., Trouten A.M., Stairley R.A., Roddy P.L., Liu C., Zhang M., Sucov H.M, & Tao G. (2023). Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury. bioRxiv.

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Western Blot Analysis of Protein Expression

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HCFs or mouse heart tissue were lysed in RIPA buffer. Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher, PI23227) according to manufacturer’s instructions. Western blot was performed as previously described.⁶³ (link) Total protein was examined using Pierce Reversible Protein Stain Kit for PVDF membranes (Thermo Fisher, PI24585) and imaged using Bio-Rad ChemiDoc Imaging System (Bio-Rad, 12003154). Target protein was detected using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher, 34577). Target band intensities were quantified using ImageJ software (National Institutes of Health). Primary Antibodies used include: FTH1 (1:1000, Santa Cruz Biotechnology, sc-376594), FTL (1:1000, Abcam, ab109373), PTGS2 (1:1000, Proteintech, 12375-1-AP), TSP1 (1:1000, Abcam, ab85762), and α-TUBULIN (1:1000, Sigma, T5168-100UL). Secondary antibodies include goat-anti-mouse (1:2500, Thermo Invitrogen, 32430) or goat-anti-rabbit (1:2500, Santa Cruz Biotechnology, sc2357) horseradish peroxidase (HRP)-conjugated antibodies.

Mohr M.E., Li S., Trouten A.M., Stairley R.A., Roddy P.L., Liu C., Zhang M., Sucov H.M, & Tao G. (2024). Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury. iScience, 27(3), 109219.

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Immunogold Labeling of Cellular Ultrastructure

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Cells were incubated with propylene oxide and then exposed to propylene oxide/Epikote. The blocks embedded with the Epon-Araldite mixture (Electron Microscopy Sciences, Hatfield, PA, USA) were sectioned for imaging under a transmission electron microscope (HITACHI-7000, Hitachi, Tokyo, Japan). For immunogold labeling, the ultrathin sections of the cells on the nickel grids were blocked with the SuperBlock^TM blocking buffer (Thermo Fisher Scientific Inc.), followed by incubation with mouse monoclonal anti-FTH1 (1:100, Santa Cruz Biotechnology, Inc., Dallas, TX, USA, sc-376594) and rabbit polyclonal anti-nuclear receptor co-activator 4 (NCOA4, 1:100, Abcam, ab222071) antibodies. The grids incubated with gold-containing goat polyclonal anti-mouse (20 nm; 1:10, Abcam, ab27242) and anti-rabbit (12 nm; 1:10, Abcam, ab105298) secondary antibodies were stained with saturated uranyl acetate and lead citrate, respectively. The images were examined under a transmission electron microscope (HITACHI-7000, Hitachi, Tokyo, Japan).

Chen H.C., Tang H.H., Hsu W.H., Wu S.Y., Cheng W.H., Wang B.Y, & Su C.L. (2022). Vulnerability of Triple-Negative Breast Cancer to Saponin Formosanin C-Induced Ferroptosis. Antioxidants, 11(2), 298.

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