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Rc210332

Manufactured by OriGene
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RC210332 is a laboratory equipment product from OriGene. It is a multi-functional device designed for use in scientific research and analysis. The core function of this product is to facilitate various laboratory tasks, such as sample preparation, measurement, and data collection. For more detailed information about the specific capabilities and intended use of RC210332, please consult the product documentation or contact OriGene directly.

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Lab products found in correlation

Hispeed plasmid maxi kit, by Qiagen (2 mentions) Rc203828, by OriGene (2 mentions) Ecorv and pmei restriction enzymes, by New England Biolabs (2 mentions)

2 protocols using rc210332

1

Generating Mutant PRKACA and PRKAR1A

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Expression plasmids encoding human PRKACA and PRKAR1A were obtained from Origene (RC210332, NM_002730; RC203828, NM_002734). The Leu206Arg mutation was introduced into PRKACA using the QuikChange site-directed mutagenesis system (Stratagene), and validated by sequencing the complete coding region. Plasmids were prepared using the HiSpeed Plasmid Maxi Kit (Qiagen). The FLAG tag was deleted in the PRKAR1A construct for the IP experiment using EcoRV and PmeI restriction enzymes (New England Biolabs).
Goh G., Scholl U.I., Healy J.M., Choi M., Prasad M.L., Nelson-Williams C., Kuntsman J.W., Korah R., Suttorp A.C., Dietrich D., Haase M., Willenberg H.S., Stålberg P., Hellman P., Åkerström G., Björklund P., Carling T, & Lifton R.P. (2014). Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors. Nature genetics, 46(6), 613-617.
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2

Generating Mutant PRKACA and PRKAR1A

Check if the same lab product or an alternative is used in the 5 most similar protocols
Expression plasmids encoding human PRKACA and PRKAR1A were obtained from Origene (RC210332, NM_002730; RC203828, NM_002734). The Leu206Arg mutation was introduced into PRKACA using the QuikChange site-directed mutagenesis system (Stratagene), and validated by sequencing the complete coding region. Plasmids were prepared using the HiSpeed Plasmid Maxi Kit (Qiagen). The FLAG tag was deleted in the PRKAR1A construct for the IP experiment using EcoRV and PmeI restriction enzymes (New England Biolabs).
Goh G., Scholl U.I., Healy J.M., Choi M., Prasad M.L., Nelson-Williams C., Kuntsman J.W., Korah R., Suttorp A.C., Dietrich D., Haase M., Willenberg H.S., Stålberg P., Hellman P., Åkerström G., Björklund P., Carling T, & Lifton R.P. (2014). Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors. Nature genetics, 46(6), 613-617.
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