Multiskan spectrum microplate spectrophotometer

Levels of ROS inside cells were tested by a common method using 2′,7′-dichlorofluorescein diacetate (DCFH-DA, Sigma-35845, dissolved in absolute ethanol) as an indicator. Cells after pre-culture were cultivated for 16–18 h in SD-His medium with FAF (0.3 g/L formic acid, 1.2 g/L acetic acid, and 0.5 g/L furfural) at 30 °C and 150 rpm. Cell pellets were washed twice with distilled water and then re-suspended in 0.5 mL of 10 mM PBS (pH 7.0) containing 10 μM DCFH-DA. After incubation at 37 °C for 60 min, fluorescence was measured by a Multiskan spectrum microplate spectrophotometer (PerkinElmer, USA).

Levels of ROS inside cells were tested by a common method using 2′,7′-dichlorofluorescein diacetate (DCFH-DA, Sigma-35845, dissolved in absolute ethanol) as an indicator. Cells after pre-culture were cultivated for 16–18 h in corresponding selective medium with FAF (0.3 g/L formic acid, 1.2 g/L acetic acid and 0.5 g/L furfural) at 30 °C and 150 rpm (OD₆₂₀ 1.0). Cell pellets were washed twice with distilled water, and then re-suspended in 0.5 mL 10 mM PBS (pH 7.0) containing 10 μM DCFH-DA. After incubation at 37 °C for 60 min, fluorescence was measured by a Multiskan spectrum microplate spectrophotometer (PerkinElmer, USA) with λ_EX 485 nm and λ_EM 525 nm [40 (link)].