Hplc grade solvents

UV spectra were obtained with a Hitachi JP/U-3010 UV spectrophotometer (Tokyo, Japan). IR spectra were acquired on a JASCO FT/IR 4200 spectrophotometer (Tokyo, Japan). All NMR spectra were recorded on a Bruker AscendTM 700 spectrometer (Billerica Middlesex County, MA, USA). Electrospray ionization source (ESI) low-resolution mass spectra were recorded on an Agilent Technologies 6120 quadrupole mass spectrometer coupled with an Agilent Technologies 1260 series HPLC (Santa Clara, CA, USA). High-resolution mass spectrometric data were collected on a JEOL JMS-700 double-focusing (B/E configuration) instrument (Tokyo, Japan). High-performance liquid chromatography was performed with a HPLC of Waters Corporation (Milford, MA, USA) equipped with a Waters 2998 photodiode array detector and Waters 1525 binary pump (Milford Worcester County, MA, USA). HPLC-grade solvents from Burdick & Jackson (Charlotte, NC, USA) were used for HPLC. NMR solvents were purchased from Cambridge Isotope Laboratories (CIL) Inc. (Andover, MA, USA).

HPLC grade solvents were obtained from VWR International (Mississauga, Ontario, Canada). The standard phospholipids; 1,2-dinonanoyl-sn-glycero-3-phosphocholine (09:0 PC), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (17:0 LPC), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC), 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC), 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDHPC), 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PAzPC) and 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PONPC) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), 1-palmitoyl-2-(5’-keto-6’-octenedioyl)-sn-glycero-3-phosphocholine (KOdiA-PC) and 1-palmitoyl-2(4’-keto-dodec-3’-ene-dioyl)-sn-glycero-3-phosphocholine (KDdiA-PC) were purchased from Cayman Chemicals (Ann Arbor, Michigan, USA). All other chemicals were purchased from Sigma Aldrich (St. Louis, Missouri, USA).

¹³C-labelled [1-¹³C]ethyl pyruvate as precursor was purchased from Eurisotop (Saint-Aubin, France). [¹³C]urea was purchased from Merck (Darmstadt, Germany). Sodium bicarbonate and 30% HCl solution were obtained from Sigma-Aldrich (St. Louis, USA). Ethyl acetate and methanol for synthesis were obtained from Merck. HPLC grade solvents were purchased from VWR (Radnor, USA).

All assay reagents, including buffer salts, ATP, and MgCl_2, were of molecular biology grade and purchased from Sigma‐Aldrich (St. Louis, MO, USA). H89 was purchased from Selleck Chem (Houston, TX, USA). PKI(5–24) was purchased from Alfa Aesar (Tewksbury, MA, USA). HPLC‐grade solvents were purchased from VWR (Radnor, PA, USA).

Chemical reactants and solvents were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France) and were used without further purification unless specified. Compound 5 was obtained according to previously reported procedures [33 (link)]. BocNH-PEG₁₀₀-NH₂ was purchased from Iris Biotech GmbH (Marktredwitz, Germany). Suppocire^TM NB and Labrafac were purchased from Gattefossé (Saint-Priest, France). Myrj^TM S40 and Myrj^TM S100 (poly(ethylene glycol) surfactants with respectively about 40 and 100 ethylene oxide units), and super-refined soybean oil were supplied by CRODA (Chocques, France). Lipoid^TM s75 was purchased from Lipoid GmbH (Ludwigshafen, Germany). SpectraPor^TM dialysis membrane of 12–14,000 MWCO was purchased from Roth Sochiel EURL (Lauterbourg, France). HPLC grade solvents were obtained from VWR Scientific (Fontenay-sous-Bois, France).
All water solutions were prepared from ultra-pure laboratory-grade water that has been filtered and purified by reverse osmosis using Millipore Milli-Q^TM cartridge system (resistivity 18.2 MΩ.cm at 25 °C, Merck, Fontenay-sous-bois, France). Thin-layer chromatography (TLC) was performed on silica gel 60 F254 (Merck). ¹H NMR spectra were recorded on a Bruker Avance 400 spectrometer (Bruker, Wissembourg, France). Chemical shifts (δ) are reported in ppm with the solvent as the internal reference.