Lysotracker red

Egg yolk phosphatidylcholine (EYPC), l-dioleoyl phosphatidylethanolamine (DOPE), and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) were purchased from NOF Corp. (Tokyo, Japan). N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) dioleoyl phosphatidylethanolamine (NBD-PE), lissamine rhodamine B-sulfonyl phosphatidylethanolamine (Rh-PE) and cholesteryl hemisuccinate (CHEMS) were purchased from Avanti Polar Lipids (Birmingham, AL, USA). Deoxycholic acid sodium salt monohydrate, oleic acid, and chlorpromazine hydrochloride were purchased from Nacalai Tesque Inc. (Kyoto, Japan). Pyranine was purchased from Tokyo Chemical Industries Ltd. (Tokyo, Japan). p-Xylene-bis-pyridinium bromide (DPX), filipin complex from Streptomyces filipinensis, OVA, and Triton X-100 were purchased from Sigma (St. Louis, MO, USA). FITC-labeled OVAlbumin (FITC-OVA) were prepared as reported previously [24 (link)]. 2-Morpholinoethanesulfomic acid sodium salt (MES) was purchased from Merck (Darmstadt, Germany). Amiloride hydrochloride dihydrate was obtained from LKT Laboratories Inc. (St. Paul, MN, USA). LysoTracker Red was purchased from Lonza (Walkersville, MD, USA). CpG-ODN (ODN-2395) was obtained from Invitrogen Corp. (San Diego, CA, USA). OVA CTL epitope peptide (SIINFEKL) was obtained from PH Japan Co., Ltd. (Hiroshima, Japan).