The following drugs were used during behavioral and/or in vivo electrophysiological experiments: the GPR55 endogenous lipid agonist PEA (0.5 or 1 μg/0.5 μL; Tocris Bioscience), selective GPR55 antagonist CID 16020046 (CID160; 1 μg/0.5 μL; Tocris Bioscience), selective CB1R antagonist Rimonabant (RIM; 0.5 μg/0.5 μL; Tocris Bioscience), selective PPARα antagonist GW 6471 (GW; 10 or 100 ng/0.5 μL; Tocris Bioscience), and selective and noncompetitive NMDA receptor antagonist MK 801 (MK801; 1 μg/0.5 μL; Tocris Bioscience). All pharmacological compounds were dissolved in dimethyl sulfoxide (DMSO) and then diluted in phosphate-buffered saline (PBS) for a final 15% DMSO in PBS solution. Intra-vHipp microinfusions were performed immediately before the start of each behavioral experiment. A total volume of 0.5 μL per side was delivered via a 28-gauge microinfusion injector over a period of 1 min following drug infusion to ensure adequate diffusion from the tip.
Cid16020046
Manufactured by Bio-Techne
Sourced in United Kingdom, United States
The CID16020046 is a laboratory device designed for DNA and RNA quantification. It utilizes spectrophotometric technology to measure the concentration and purity of nucleic acid samples. The core function of this product is to provide accurate and reliable measurements of DNA and RNA in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
O 1602, by Bio-Techne (4 mentions)
Am251, by Bio-Techne (3 mentions)
Mk 801, by Bio-Techne (2 mentions)
Sb366791, by Bio-Techne (2 mentions)
Casy cell counter, by Roche (2 mentions)
Am630, by Bio-Techne (2 mentions)
Rimonabant rim, by Bio-Techne (1 mentions)
Lh 21, by Cayman Chemical (1 mentions)
Anandamide, by Bio-Techne (1 mentions)
Capsaicin, by Bio-Techne (1 mentions)
Clobazam, by Merck Group (1 mentions)
Valproic acid, by Merck Group (1 mentions)
Capsazepine, by Bio-Techne (1 mentions)
Egm 2, by Merck Group (1 mentions)
Penicillin streptomycin l glutamine heparin, by Thermo Fisher Scientific (1 mentions)
U0126, by Cell Signaling Technology (1 mentions)
Ebm 2, by Lonza (1 mentions)
Egm 2, by Lonza (1 mentions)
Nnc 55 0396, by Bio-Techne (1 mentions)
Lysophosphatidylinositol lpi, by Merck Group (1 mentions)
15 protocols using cid16020046
1
Pharmacological Modulation of Hippocampal Function
2
Pharmacological Treatment of Obese Pre-Diabetic Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LH-21 was purchased from Cayman (Cayman Chemical, Ann Harbor, MI), dissolved in 100% ethanol and stored in 30 mg/ml aliquots. For daily injections, fresh dilutions were prepared in vehicle (5% Tween 80 in saline; final ethanol concentration 1%). Either vehicle or LH-21 were i.p. injected daily for two weeks in the obese pre-diabetic mice that had been fed a HFD for 15 weeks. An effective dosage of 3 mg/Kgday−1 LH-21 was used to treat animals as previously described12 (link). For the pharmacological experiments in naïve healthy mice LH-21 was prepared and administered as above. CID16020046 was purchased from Tocris (Tocris Bioscience, Bristol, UK), dissolved in 100% DMSO and stored in 30 mg/ml aliquots. In the experiments, CID16020046 was dissolved in saline and i.p. injected 15 min before LH-21 injections.
3
Endocannabinoid Modulation of ECFC Growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three different ECFC donors were seeded in 6-well plates (Nalge Nunc, Rochester, NY) in EGM-2 at a density of 3,000 c/cm2 and allowed to adhere for 24 hours. Subsequently, cells were subjected to growth factor reduced medium with or without AEA and different endocannabinoid receptor agonists and antagonists as indicated. The substances anandamide, CID16020046, SB366791 and Capsaicin were obtained by Tocris Bioscience (Northpoint, Avonmouth, Bristol, UK). SKM4-45-1, AM251, SR144528 (Cayman Chemical Europe, Tallinn, Estonia). After 48 hours treatment cells were harvested and cell numbers analyzed by a Casy cell counter (Roche, Mannheim, Germany).
4
Cannabinoids and Anticonvulsant Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CBDV, CBG and Δ9‐THCV were purchased from THC Pharm GmbH (Frankfurt, Germany). CBGA was purchased from THC Pharm GmbH and Epichem (Bentley, Australia). CBGV was purchased from Toronto Research Chemicals Inc. (Ontario, Canada). CBDVA and CBGVA were synthesized by Professor Michael Kassiou at the University of Sydney (Australia). Clobazam and valproic acid were purchased from Sigma‐Aldrich Co. (St. Louis, USA). When available, analytical standards were purchased from Novachem Pty Ltd (Heidelberg West, Australia). For in vitro experiments, drugs were prepared as stocks in DMSO. For acute administration, drugs were prepared fresh on the day of the experiment. CBDV, CBG, CBGV and Δ9‐THCV were prepared in ethanol:Tween 80:saline (1:1:18). CBGV (100 mg·kg−1) was a suspension, with CBDV (60 mg·kg−1) and CBG (30 mg·kg−1) near solubility limits. CBDVA, CBGA, CBGVA and Clobazam were prepared as solutions in vegetable oil. Drugs were administered as an intraperitoneal injection in a volume of 10 ml·kg−1. ML‐186 was purchased from Enamine (Kyiv, Ukraine). CID16020046 and lysophosphatidylinositol were purchased from Tocris Biosciences (Bristol, United Kingdom) and Sigma‐Aldrich (St. Louis, USA).
5
Investigating CBD's Modulation of Cocaine Effects
6
Endothelial Cell Functional Assay with LPI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ECFCs from three different cord blood donors, HUVECs and peripheral blood ECFC were seeded in 24-well plates (Nalge Nunc, Rochester, NY, USA) in EGM-2 at a density of 3000 cells/cm2 and allowed to adhere for 24 h. Subsequently, cells were subjected to growth factor-reduced medium [EBM-2 (Lonza) containing 2% FBS and 1% penicillin/streptomycin/L-glutamine/heparin (Life Technologies) without the addition of EGM-2 growth factor supplements] with or without different concentrations of LPI (Sigma) and/or endocannabinoid receptor antagonists: CID16020046 (Tocris Bioscience, Northpoint, Avonmouth, Bristol, UK) and AM251, SR144528 (both Cayman Chemical Europe, Tallinn, Estonia). A 30 min treatment with 10 μM U0126 (Cell Signaling, Cambridge, UK) was also tested. After 48 h, treated cells were harvested and the cell number was counted by a Casy cell counter (Roche, Mannheim, Germany). Nine independent experiments per group were performed in triplicate.
7
Synthesis and Administration of CBGA Methyl Ester
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CBGA methyl ester was synthesized by Acme Bioscience, Inc (Palo Alto, USA), with > 98% purity. Olivetolic acid was purchased from Toronto Research Chemicals Inc. (North York, CAN), with >97% purity. Compounds were prepared fresh on the day of the experiment as solutions in ethanol:Tween80:saline (1:1:18) and administered as an intraperitoneal (i.p.) injection in a volume of 10 mL/kg. CID16020046 and NNC 55-0396 were purchased from Tocris Biosciences (Bristol, GBR) and Cayman Chemical (Ann Arbor, USA), respectively. Lysophosphatidylinositol (LPI) was purchased from Sigma-Aldrich (St. Louis, USA).
8
Cannabinoid Receptor Ligand Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CBG and CBDV were kindly gifted by STI pharmaceuticals. Both compounds were dissolved in 100% ethanol to 10 mM and were stored at −20°C. AM251, AM630, GW6471, GW9962, O1918, CID16020046, SB366791 (Tocris, United Kingdom) were dissolved in dimethyl sulfoxide as stock solutions of 10 mM. (S)-WAY100135 was dissolved in deionized water. Antagonists were stored at −20°C and dilutions were made fresh as required.
9
Pharmacological Modulation of GPR55 and GPR119
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2-[[2-(4-Bromophenyl)-6-methyl-4-pyrimidinyl]amino]ethanol (AS1269574), 5-Methyl-4-[(1R,6R)-3-methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl]-1,3-benzenediol (O1602), 4-[4,6-Dihydro-4-(3-hydroxyphenyl)-3-(4-methylphenyl)-6-oxopyrrolo[3,4-c]pyrazol-5(1H)-yl]benzoic acid (CID16020046) and G-protein antagonist peptide were purchased from Tocris Bioscience (Bristol, UK). All drugs were freshly suspended before each experiment in normal saline solution containing 10% of Tween 20. Drugs were selected based on their commercial availability as well as for their selectivity for GPR55 and GPR119 receptors. AS1269574 is a selective GPR119 agonist with a pEC50 = 5.6 [43 (link)], O1602 is a GPR55 agonist with a pEC50 = 7.9–8.9 [44 (link)], CID16020046 is a selective GPR55 antagonist with a pA2 = 7.3 [22 (link)] and G-protein antagonist peptide is a blocker of the activation of G proteins by receptors [33 (link)]. The drug doses were chosen from pilot experiments under our conditions.
10
Investigating Immune Cell Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
O-1602 and CID16020046 were purchased from Tocris Bioscience (Ellisville, MO, USA). R-phycoerythrin (PE)-conjugated mouse anti-human Mac-1 (clone ICRF44; BD) antibody (Cat No. 555388) and PE-conjugated mouse IgG isotype control (clone MOPC-21) antibody (Cat No. 555749) were obtained from BD Biosciences (San Diego, CA, USA). Various signal pathway inhibitors were purchased from EMD Serono (Rockland, MA, USA) and Sigma-Aldrich (St. Louis, MO, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!