Goat anti rabbit cross adsorbed alexa fluor 546 conjugate

Manufactured by Thermo Fisher Scientific

The Goat anti-rabbit cross-adsorbed Alexa Fluor 546 conjugate is a secondary antibody reagent used in fluorescence-based detection and imaging applications. The conjugate consists of goat-derived antibodies that are specific to rabbit primary antibodies, and are covalently linked to the Alexa Fluor 546 fluorescent dye. This reagent allows for the indirect detection and visualization of target proteins or other biomolecules that have been labeled with a rabbit primary antibody.

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Lab products found in correlation

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Close spelling variants of this product

Alexa Fluor 546 goat anti-rabbit Alexa Fluor 546-conjugate Alexa Fluor 546 anti-rabbit Goat anti-rabbit Alexa 546 Alexa Fluor goat anti-rabbit Alexa 546 anti-goat Alexa Fluor 546 Alexa Fluor conjugates Alexa Fluor anti-rabbit Alexa 546

2 protocols using goat anti rabbit cross adsorbed alexa fluor 546 conjugate

Embryonic Immunohistochemistry Protocol

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Embryos were fixed in 4% paraformaldehyde (PFA; Sigma), and immunohistochemistry was performed as previously described (Prince et al., 1998 (link)) using the following primary antibodies: rabbit polyclonal anti-Cdh1 (E-Cadherin; 1:100; GeneTex GTX100443), rabbit polyclonal Cdh2 (N-Cadherin; 1:200; GeneTex GTX125885), rabbit polyclonal anti-Sox10 (1:250; Genetex GTX128374) and rabbit polyclonal anti-Caspase-3 (1:100; Millipore #AB3623). The following secondary antibodies were used: goat-anti mouse highly cross-adsorbed Alexa Fluor Plus 488 (Molecular Probes A32723), goat anti-rabbit highly cross-adsorbed Alexa Fluor 488 (Molecular Probes A11034), goat anti-rabbit cross-adsorbed Alexa Fluor 546 conjugate (Molecular Probes A11010), and anti-GFP Alexa Fluor 488 conjugate (Molecular Probes A21311). Embryos were then cleared in 80% glycerol, deyolked and flat-mounted.

Ahsan K., Singh N., Rocha M., Huang C, & Prince V.E. (2019). Prickle1 is required for EMT and migration of zebrafish cranial neural crest. Developmental biology, 448(1), 16-35.

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Immunohistochemical Analysis of Embryonic Sox10 and Myosin Expression

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Embryos were fixed in 4% paraformaldehyde (PFA; Sigma) and
immunohistochemistry was performed as previously described (Prince et al., 1998 (link)) using the following primary
antibodies: rabbit anti-Sox10 antibody (1:100, GeneTex, GTX128374), mouse
anti-myosin heavy chain (1:100, Developmental Studies, Hybridoma bank, IA, USA,
A4.1025). The following secondary antibodies were used: goat-anti mouse highly
cross-adsorbed Alexa Fluor Plus 488 (Molecular Probes A32723), goat anti-rabbit
cross-adsorbed Alexa Fluor 546 conjugate (Molecular Probes A11010). Embryos were
also stained with DAPI (Thermo Fisher, R37606) and then mounted in 1% low
melting agarose for imaging.

Rocha M., Kushkowski E., Schnirman R., Booth C., Singh N., Beadell A, & Prince V.E. (2021). Zebrafish Cdx4 regulates neural crest cell specification and migratory behaviors in the posterior body. Developmental biology, 480, 25-38.

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