Luna c18 column 2 x 50 mm

The LC-MS instrumentation included an API 5000 Triple Quadrupole Mass Spectrometer (Applied Biosystems, Foster City, CA) with Turboion Spray source coupled with an Agilent 1200 Liquid Chromatograph (Santa Clara, CA). A Phenomenex Luna C18 column (2 X 50 mm) was used (Torrance, CA) with a flow rate of 1 mL/min. Mobile phases A: 0.1% formic in water and B: 0.1% formic acid in methanol were used with an initial composition of 95:5 A:B held for 1 min followed by a linear gradient to 10:90 A:B over 4 min, and held for 3 min. The ion source was operated in positive ion mode with a source temperature of 650 °C and voltage spray of 4500 V. The transition monitored for quantitation of NBBS was m/z 214.1 → 157.8.

The LC-MS instrumentation included an API 5000 Triple Quadrupole Mass Spectrometer (Applied Biosystems, Foster City, CA) with Turboion Spray source coupled with an Agilent 1200 Liquid Chromatograph (Santa Clara, CA). A Phenomenex Luna C18 column (2 X 50 mm) was used (Torrance, CA) with a flow rate of 1 mL/min. Mobile phases A: 0.1% formic in water and B: 0.1% formic acid in methanol were used with an initial composition of 95:5 A:B held for 1 min followed by a linear gradient to 10:90 A:B over 4 min, and held for 3 min. The ion source was operated in positive ion mode with a source temperature of 650 °C and voltage spray of 4500 V. The transition monitored for quantitation of NBBS was m/z 214.1 → 157.8.