Phenylephrine,9 (link) ACh,9 (link) RN1734,7 (link) GSK1016790A,13 (link) NG-nitro-L-arginine (L-NNA),8 (link) indomethacin,8 (link) and apamin8 (link) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-KCa2.3 (catalog No. APC-025) and anti-TRPV4 (catalog No. ACC-034) antibodies were purchased from Alomone Labs.
Ng nitro l arginine l nna
Manufactured by Merck Group
Sourced in United States
NG-nitro-L-arginine (L-NNA) is a laboratory reagent used for research purposes. It is a selective and potent inhibitor of nitric oxide synthase, an enzyme involved in the production of nitric oxide. L-NNA is commonly used in scientific research to study the role of nitric oxide in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Gsk1016790a, by Merck Group (1 mentions)
Rn 1734, by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Atropine sulphate, by Merck Group (1 mentions)
Granisetron hydrochloride, by Merck Group (1 mentions)
Hemin, by Merck Group (1 mentions)
Zinc protoporphyrin 9, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Atropine sulfate, by Merck Group (1 mentions)
Forskolin, by Bio-Techne (1 mentions)
Okadaic acid, by Bio-Techne (1 mentions)
Chelerythrine chloride, by Merck Group (1 mentions)
Charybdotoxin, by Merck Group (1 mentions)
Glibenclamide, by Merck Group (1 mentions)
Trifluoperazine dihydrochloride, by Merck Group (1 mentions)
Carbamoylcholine chloride, by Merck Group (1 mentions)
Tram 34, by Bio-Techne (1 mentions)
Bay k8644, by Merck Group (1 mentions)
Rp 8 br pet cgmps, by Bio-Techne (1 mentions)
Kt5823, by Merck Group (1 mentions)
5 protocols using ng nitro l arginine l nna
1
Studying Vascular Endothelial Function
2
Pharmacological Effects of Vasoactive Agents
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The following drugs were used: atropine sulphate and granisetron hydrochloride dissolved in saline (Sigma Chemical Co., St. Louis, Missouri, United States). Zinc protoporphyrin IX and hemin were dissolved in 0.1 N NaOH and equilibrated to a pH of 7.4 with HCl (Sigma Chemical Co., St. Louis, Missouri, United States). Tricarbonyl Chloro(glycinato)ruthenium (II) (CORM-3) and NG-nitro-L-Arginine (L-NNA) were dissolved in distilled water (Sigma Chemical Co., St. Louis, Missouri, United States). In in vivo studies, vehicle-treated rats received the same amount of vehicle as did drug-treated animals. In in vitro experiments, vehicle-treated preparations were exposed to the same amount of vehicle as drug-treated preparations.
3
Vasodilator Evaluation in Murine Preparations
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AF B1, a nitric oxide (NO) synthase inhibitor [NG-nitro-L-arginine (L-NNA)], and atropine sulfate were obtained from Sigma-Aldrich (Merck KGaA). A 2 mg/ml stock solution of AF was prepared in dimethyl sulfoxide (which does not directly affect murine-isolated smooth muscle preparations) and stored at 0–4°C. Other drugs were dissolved in ethanol and deionized water (1:1) as 1 mg/ml stock solutions, and were subsequently diluted in deionized water before use.
4
Pharmacological Modulation of Signaling Pathways
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Acetylcholine (ACh), carbamoylcholine chloride (carbachol), Bay K8644, trifluoperazine dihydrochloride, apamin, charybdotoxin (ChTX), glibenclamide, quinine, verapamil, papaverine dihydrochloride, H-89 dihydrochloride hydrate, KT-5823, chelerythrine chloride, (+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclohexanecarboxamide dihydrochloride monohydrate (Y-27632), dibutyryl-cAMP (d-cAMP), d,l -propargylglycine (PAG), aminooxyacetic acid hemihydrochloride (AOAA), tetrodotoxin (TTX) and NG-nitro-l arginine (l -NNA) were obtained from Sigma (Madrid, Spain). Tram-34, 1H-[1,2,4]oxadiazolo [4,3-α]quinoxalin-1-one (ODQ), Rp-8-Br-PET-cGMPS, okadaic acid and forskolin were purchased from Tocris (Madrid, Spain). All chemicals were of analytical grade. Bay K8644 was dissolved in ethanol. apamin was diluted in acetic acid. glibenclamide, Tram-34, forskolin (Fk), okadaic acid and ODQ were prepared in dimethyl sulfoxide. All other chemicals were dissolved in distilled water.
5
Pharmacological Modulation of Cerebellar Signaling
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The reagents included picrotoxin, D-(-)-2-amino-5-phosphonopentanoic acid (D-APV), NG-Nitro-L-arginine (L-NNA), nitric oxide synthase (NOS) inhibitor, nicotine, methyllycaconitine citrate hydrate (MLA), and dihydro-β-erythroidine hydrobromide (DβEH) bought from Sigma-Aldrich (Shanghai, China). All these chemicals were dissolved in solution and kept in frozen aliquots. For experiments with NOS inhibitor, L-NNA, cerebellar surface was superfused with 200 μM L-NNA for 1 h before recordings were began. The drugs were dissolved in ACSF and applied directly onto the cerebellar surface by a peristaltic pump (0.5 ml/min).
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