Female BLT-NOD-scid IL2Rg−/− (NSG) mice (The Jackson Laboratory) were housed in a pathogen-free facility at Massachusetts General Hospital and reconstituted with human tissue as described (Brainard et al., 2009 (link)). Briefly, sublethally irradiated mice were transplanted under the kidney capsule with 1 mm3 fragments of human fetal liver and thymus, and injected intravenously with purified CD34+ human fetal liver cells to generate humanized BLT mice. Human immune cell reconstitution was monitored by flow cytometry at week 12 and week 17 post-implantation and considered sufficient if at least 200 CD4+ T cells/μl were present in peripheral blood. BLT mice showed no clinical signs of GvHD at any time during the experiment. Mice were infected 3 times intravenously, in a tail vein, at day 0, 2 and 4 in a total volume of 200 μL PBS, each time. Animals were euthanized 5 to 7 days after the first HIV infection and tissues were harvested for flow cytometry analysis.
Female blt nod scid il2rg nsg mice
Manufactured by Jackson ImmunoResearch
The Female BLT-NOD-scid IL2Rg−/− (NSG) mice are a type of immunodeficient mouse model. They have a deficiency in the interleukin-2 receptor gamma chain (IL2Rg), which leads to a lack of mature T cells, B cells, and natural killer cells. These mice are often used in biomedical research, particularly for the study of human immune system development and function.
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2 protocols using female blt nod scid il2rg nsg mice
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Humanized BLT Mouse Model for HIV
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Humanized BLT Mouse Model for HIV
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Female BLT-NOD-scid IL2Rg−/− (NSG) mice (The Jackson Laboratory) were housed in a pathogen-free facility at Massachusetts General Hospital and reconstituted with human tissue as described (Brainard et al., 2009 (link)). Briefly, sublethally irradiated mice were transplanted under the kidney capsule with 1 mm3 fragments of human fetal liver and thymus, and injected intravenously with purified CD34+ human fetal liver cells to generate humanized BLT mice. Human immune cell reconstitution was monitored by flow cytometry at week 12 and week 17 post-implantation and considered sufficient if at least 200 CD4+ T cells/μl were present in peripheral blood. BLT mice showed no clinical signs of GvHD at any time during the experiment. Mice were infected 3 times intravenously, in a tail vein, at day 0, 2 and 4 in a total volume of 200 μL PBS, each time. Animals were euthanized 5 to 7 days after the first HIV infection and tissues were harvested for flow cytometry analysis.
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