The heart tissues were then embedded in OCT (Optimum Cutting Temperature) compound and were cut into approximately 5 µm thick sections. Then, the sections were subjected to the TUNEL (Roche, Mannheim, Germany) staining to detect cardiomyocyte apoptosis according to the kit's manual. The section images were taken by a Nikon ECLIPSE Ti microscope (Olympus BX51, Tokyo, Japan).
Eclipse ti microscope
Manufactured by Olympus
Sourced in Japan
The ECLIPSE Ti microscope is a high-performance inverted microscope designed for advanced imaging and analysis. It features a stable, ergonomic design and supports a wide range of observation techniques, including phase contrast, fluorescence, and differential interference contrast (DIC) imaging.
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Lab products found in correlation
6 protocols using eclipse ti microscope
1
Cardiomyocyte Apoptosis Detection
2
Quantitative Microscopic Tissue Analysis
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Stained tissue sections were visualized by an Olympus BX51 or a Nikon ECLIPSE Ti microscope, and photographed with a color camera (Olympus DP73) or cooled CCD monochrome cameras (Olympus XM10 and Photometrics CoolSNAP HQ2). Digital images were acquired with imaging software (Olympus cellSens Dimension and Nikon NIS-Elements AR). For quantification, images were acquired from 5 microscopic fields per tissue with a 20X objective lens, and cells were quantified using the counting function of the NIH ImageJ software. Typical ranges of total cell count per tissue were 400 - 600 in the basal layer and 1,500 - 2,000 in the suprabasal layers.
3
Apoptosis Analysis in Heart Tissue
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The heart tissues were harvested and embedded in optimal cutting temperature compound, and then, they were cut into approximately 5‐μm thick sections. The sections were firstly stained with cTNI and then were subjected to the TUNEL (Roche, Basel, Switzerland) staining to detect cell apoptosis. The section images were taken by a Nikon ECLIPSE Ti microscope (Olympus BX51, Japan).
4
Immunofluorescence Analysis of Bone Marrow Macrophages
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BMMs were seeded onto bovine cortical bone slices in a 12-well plate at a density of 3 × 105 cells/well and incubated for 24 h. The glass coverslips with BMMs were washed three times with PBS, and then the cells were fixed with 4% PFA for 30 min and permeabilized with Triton X-100 for 10 min at room temperature. After blocking with 5% bovine serum albumin for 30 min, the BMMs were incubated with primary antibodies against ALDH1A1 (1:500) and p65 (1:500) overnight. BMMs were then incubated with Alexa Fluor 530-conjugated IgG secondary antibodies for 1 h and stained with 4′,6-diamidino-2-phenylindole (DAPI) for 5 min. Images were analyzed under a Nikon Eclipse Ti microscope (Tokyo, Japan) and Olympus FV1200 microscope (Tokyo, Japan).
5
Cardiomyocyte Apoptosis Detection via TUNEL
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After 2 hours of reperfusion, the heart was removed. The heart tissues were then embedded in optimal cutting temperature compound and approximately 5‐µm thick sections were cut. Terminal deoxynucleotidyl transferase‐mediated dUTP nick end labelling (TUNEL) (Roche, Mannheim, Germany) staining was used to detect cardiomyocytes apoptosis according to the kit's manual. The section images were taken by a Nikon ECLIPSE Ti microscope (Olympus BX51, Japan).
6
Myocardial Apoptosis Analysis via TUNEL
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Heart tissues were embedded in OCT for sectioning (5 µm) . Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Roche Diagnostics) was used to examine myocardial apoptosis according to the manufacturer's instructions. Images were collected with a Nikon ECLIPSE Ti microscope (Olympus BX51, Tokyo, Japan).
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