Axon digidata 1550 digitizer

Manufactured by Molecular Devices

Sourced in United States

The Axon Digidata 1550 Digitizer is a high-performance data acquisition system designed for electrophysiology applications. It features 16 analog input channels, 8 digital input/output channels, and can sample at rates up to 500 kHz. The device offers precise data capture and low-noise performance to support a wide range of electrophysiology experiments.

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (1 mentions) Pclamp 10, by Molecular Devices (1 mentions) Axopatch 200b amplifier, by Molecular Devices (1 mentions) Origin 9, by OriginLab (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Multiclamp 700b amplifier, by Molecular Devices (1 mentions)

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Digidata 1550 (47 citations) Digidata 1550 digitizer (14 citations) Axon Digidata 1550 (11 citations)

6 protocols using axon digidata 1550 digitizer

Electrophysiological characterization of KCNQ1-KCNE3 channels

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Chinese hamster ovary (CHO) cells, cultured in DMEM-F12 (Gibco) with 10% FBS and L-glutamine, were transfected with the hKCNQ1_EM and KCNE3 expression plasmid (1:2 mass ratio) with lipofectamine 3000 reagent (Thermo Fisher Scientific). A medium exchange was carried out after 4hr incubation with lipofectamine reagent. 48 hrs following transfection, the media was replaced with bath solution and experiments were performed at room temperature using whole-cell patch clamp techniques with polished borosilicate glass pipettes with resistance between 2-4 MΩ. All recordings were carried out using pClamp10.5 software (Molecular Devices), an Axopatch 200B amplifier (Molecular Devices), and an Axon digidata 1550 digitizer (Molecular Devices). Data were filtered at 1 kHz and digitized at 10 kHz.
The bath solution contains 10 mM HEPES-Na pH 7.2, 20 mM KCl, 130 mM NaCl, 5 mM glucose, 2 mM MgCl₂ and 1 mM CaCl₂, and the pipette solution was 10 mM HEPES-Na pH 7.2, 50 mM KF, 100 mM KCl, 5 mM EGTA. Data was processed using Origin 9 (OriginLab Corp.).

Sun J, & MacKinnon R. (2019). Structural basis of human KCNQ1 modulation and gating. Cell, 180(2), 340-347.e9.

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Assessing Sciatic Nerve Function

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The electrophysiological test was performed by Axon Digidata 1550 Digitizer, Molecular Devices as previously described [29 (link)–31 (link)]. In brief, 8 weeks after the implantation, the animals were deeply anaesthetized by 3% sodium pentobarbital solution (30 mg/kg, i.p.). One pair of stimulating electrodes was placed at the paraspinal site percutaneously where the sciatic nerve forms most proximally. The recording electrode needle was inserted into the lateral gastrocnemius muscle to recording the compound muscle action potentials (CMAPs) among consecutive stimulations. The recorded latency and amplitude of CMAP were analyzed.

Yang Z., Yang Y., Xu Y., Jiang W., Shao Y., Xing J., Chen Y, & Han Y. (2021). Biomimetic nerve guidance conduit containing engineered exosomes of adipose-derived stem cells promotes peripheral nerve regeneration. Stem Cell Research & Therapy, 12, 442.

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Electrophysiological Assessment of Sciatic Nerve

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Two hours after the final behavioral analyses, each mouse was anesthetized by an intraperitoneal injection of 12 mg/ml tribromoethanol (180 mg/kg body weight) and subjected to electrophysiological tests following a previously described protocol (Wang et al., 2014 (link)). Briefly, the sciatic nerve was re-exposed, a pair of stimulating electrodes (13 mm long, 0.5 mm in diameter) was inserted 3 mm proximal to the crushed site to stimulate the sciatic nerve, and a pair of needle electrodes (13 mm long, 0.5 mm in diameter) was inserted subcutaneously into the middle of the intrinsic foot muscle to record the compound muscle action potential (CMAP) with a set of electrophysiological recorders (Axon Digidata 1550 Digitizer, Molecular Devices). The amplitude and latency of each test were analyzed to determine the nerve conduction strength and nerve conduction speed, respectively.

Li L., Li Y., Fan Z., Wang X., Li Z., Wen J., Deng J., Tan D., Pan M., Hu X., Zhang H., Lai M, & Guo J. (2019). Ascorbic Acid Facilitates Neural Regeneration After Sciatic Nerve Crush Injury. Frontiers in Cellular Neuroscience, 13, 108.

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Nerve Conduction Evaluation after Injury

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To assess nerve conduction of the injured nerve, the mice were anesthetized with 180 mg/kg tribromoethanol post 3 weeks after surgery, and electrophysiological examination was performed as previously reported [14 (link), 37 (link)]. Briefly, a pair of needle electrodes were inserted to stimulate the sciatic nerve 3 mm proximal to the crush site, and a pair of recording electrodes were inserted subcutaneously into the middle of the intrinsic foot muscle. After a single stimulation with a strength of 10 mA and a duration of 0.1 ms, the amplitude and latency of the compound muscle action potential were recorded with a set of electrophysiological recorders (frequency of 20 Hz and pulse width of 0.1 ms) (Axon Digidata 1550 Digitizer, Molecular Devices, Sunnyvale, CA, USA). Immediately after the electrophysiological testing, the mice were transcardially perfused with 4% PFA, and the gastrocnemius muscles and sciatic nerves were collected for histomorphometry examination, Western blotting or immunofluorescence.

Zou Y., Zhang J., Xu J., Fu L., Xu Y., Wang X., Li Z., Zhu L., Sun H., Zheng H, & Guo J. (2021). SIRT6 inhibition delays peripheral nerve recovery by suppressing migration, phagocytosis and M2-polarization of macrophages. Cell & Bioscience, 11, 210.

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Muscle Activity Recording in Biceps Femoris

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The OFUS focus was aligned with the primary motor cortex (ML: 1.5, AP: 0.5). To record the muscle activity, the needle electrode was inserted subcutaneously into the hind limb biceps femoris muscle and the ground electrode was inserted into the tail. The control group was recorded on the trunk ipsilateral to the stimulation site. EMG signals were recorded by a Multi-Clamp 700B amplifier (Molecular Devices), filtered at 1 to 5000 Hz, digitized with an Axon DigiData 1,550 digitizer (Molecular Device), and filtered by a noise eliminator (D-400, Digitimer). EMG signal was filtered with bandpass filter at 0.5 ~ 500 Hz and full-wave rectification. Then the envelope of the processed signal was plotted.

Li Y., Jiang Y., Lan L., Ge X., Cheng R., Zhan Y., Chen G., Shi L., Wang R., Zheng N., Yang C, & Cheng J.X. (2022). Optically-generated focused ultrasound for noninvasive brain stimulation with ultrahigh precision. Light, Science & Applications, 11, 321.

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Electrophysiological Assessment of Sciatic Nerve

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The electrophysiological test was performed as per the previous studies [35 (link)–37 (link)]. The animals were anesthetized with an intraperitoneal injection of 12 mg/mL tribromoethanol (90 mg/kg body weight). Then the involved sciatic nerve was exposed, stimulating electrodes were applied to the host nerve trunk 3 mm proximal to the injury site, and a pair of electrodes was placed in the intrinsic foot muscle to record the compound muscle action potential (CMAP) with a set of electrophysiological record (Axon Digidata 1550 Digitizer, Molecular Devices). The amplitude and latency of each animal were recorded at 10 attempts, then the highest amplitude and mean latency were measured and analyzed.

Xu J., Wen J., Fu L., Liao L., Zou Y., Zhang J., Deng J., Zhang H., Liu J., Wang X., Zuo D, & Guo J. (2021). Macrophage-specific RhoA knockout delays Wallerian degeneration after peripheral nerve injury in mice. Journal of Neuroinflammation, 18, 234.

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