BALB/c female mice (aged 4 weeks) were purchased from Shanghai Laboratory Animal Center (Shanghai, P.R. China). All animal experiments were approved by the Institutional Animal Care and Use Committee of Harbin Medical University. The animal experiments were carried out in accordance with EU Directive 2010/63/EU. Mice housed under identical conditions were allowed free access to a standard diet and tap water with a 12-h light:12-h dark cycle. Four-week-old female mice were injected with 1 × 105 stably transfected pcDNA3.1(+)-mHO-1 cells or pcDNA3.1(+) in the left mammary fat pad17 (link). Tumor size was measured using a caliper every 2 days. Both the maximum (L) and minimum (W) lengths of the tumors were measured, and the tumor size was calculated as (L × W2)/2. After 24 days, the mice were sacrificed and photographed. Four-week-old female mice were injected with 1 × 106 stably transfected pcDNA3.1(+)-mHO-1 or pcDNA3.1(+) cells via the tail vein. After 17 days, the mice were sacrificed and photographed. The lungs were harvested for paraffin embedding, sectioning, and hematoxylin and eosin (H&E) staining. Five animals were included in each group.
Female balb c mice
Manufactured by Shanghai Laboratory Animal Center
Sourced in China
Female BALB/c mice are a common laboratory mouse strain used in a variety of research applications. They are a widely used model organism for studying immune function, cancer, and other biological processes.
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Lab products found in correlation
Female c57bl 6 mice, by Shanghai Laboratory Animal Center (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Lab diet 5001, by Purina (1 mentions)
Male sprague dawley rats, by Shanghai Laboratory Animal Center (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Contour glucose meter, by Bayer (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Streptozotocin (stz), by Bayer (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Beyotime (1 mentions)
Trypsin, by Beyotime (1 mentions)
Female nude mice, by Shanghai Laboratory Animal Center (1 mentions)
21 protocols using female balb c mice
1
Mouse Xenograft Model for Tumor Growth
2
Evaluation of Anti-NMDAR Antibodies in HSV-1 Infection
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All animal procedures were approved by our Institutional Animal Care and Use Committee. Balb/c female mice, ~ 12 weeks of age, were purchased from Shanghai Laboratory Animal Center. Six mice were inoculated intranasally with HSV-1 for 2 weeks. 1 × 106 plaque-forming units of HSV-1 (strain 17 syn +)6 were applied once daily. Blood/serum was collected at 3, 6, and 8 weeks post-inoculation and tested for anti-NMDAR antibodies through a cell-based assay as previously reported [8 (link)]. HEK293 cells transfected with subunits of NMDA receptor were fixed in 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, and co-incubated with mouse sera (diluted 1:100) along with a rabbit monoclonal antibody against rabbit polyclonal antibodies against NR2B (1:200, Upstate Biotechnology) followed by the appropriate fluorescent secondary antibodies. Exosomes were isolated from pooled sera, the structures of exosomes were characterized by EM, and the concentration of exosomes was counted by NTA.
3
Murine Tumor Cell Culture Protocol
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Murine colon cancer cell line CT26, murine B16-F10 melanoma cells, and dendritic cell line DC2.4 were obtained from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China). The cells were cultured at 37 °C under 5% CO2 in complete medium consisting of RPMI-1640 medium or DMEM, supplemented with 10% (v/v) heat-inactivated fetal bovine serum, penicillin (100 μg/mL), and streptomycin (100 μg/mL). Female BALB/c mice (6–8 weeks) and female C57BL/6 mice (6–8 weeks) were obtained from Shanghai Laboratory Animal Center (SLAC) Co., Ltd. (Shanghai, China), and housed at the SPF care facility with sterilized food pellets and distilled water under a 12-h light/dark cycle. The mice were allowed to adapt to their environment for one week before the experiments. All the animal experimental procedures were approved by Institutional Animal Care and Use Committee (IACUC), Shanghai Institute of Materia Medica (SIMM), Chinese Academy of Sciences. (Note: SIMM is an institution with AAALAC International accreditation.)
4
Allergic Model in Female BALB/c Mice
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Female BALB/c mice (5 weeks old, specific pathogen-free) were approved by Shanghai Laboratory Animal Center. The animals were raised in an air-conditioned room and allowed free access to tap water and food (without milk protein).
The mice model of allergy is carried out according to the method previously described (Ma, Lozano-Ojalvo, Chen, Lopez-Fandiño & Molina, 2015 ) with some modifications. For sensitization, intraperitoneal injection of mice was carried with 200 μg BLG and (1 mmol/L acrolein) Acrolein-BLG (n = 10) adsorbed in alum adjuvant (2 mg ml−1) on days 7, 14, 21 and 28, as described inFig. 5 A. After sensitization stage, mice were distributed into three groups, namely; PBS, BLG and Acrolein-BLG in order to observe the changes in allergenicity. On days 31, blood samples of each samples were collected after sacrifice. Sera were obtained from the blood after centrifugation and were stored at 20 ℃ until analyzed. The level of BLG-specific IgE and IgG1 in serum was determined by indirect ELISA, while the concentrations of histamine and mMCP-1 were measured using sandwich ELISA kits.
The mice model of allergy is carried out according to the method previously described (Ma, Lozano-Ojalvo, Chen, Lopez-Fandiño & Molina, 2015 ) with some modifications. For sensitization, intraperitoneal injection of mice was carried with 200 μg BLG and (1 mmol/L acrolein) Acrolein-BLG (n = 10) adsorbed in alum adjuvant (2 mg ml−1) on days 7, 14, 21 and 28, as described in
5
BALB/c Mice Husbandry and Experimentation
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A total of 94 female BALB/c mice (age, 6–8 weeks; weight, 20–30 g) were purchased from Shanghai Laboratory Animal Center (Shanghai SLAC Laboratory Animal Co., Ltd.). The Animal Research Center of the First Affiliated Hospital of Nanchang University provided a temperature- and humidity-controlled condition (24°C with 12 h light/dark cycle, 35–40% humidity) and ad libitum access to a standard rodent diet and water (LabDiet-5001; Purina Mills, Inc.) for all mice. All animal experiments were conducted according to the standards of the ‘Guide for the Care and Use of Laboratory Mice’ (Institute of Laboratory Animal Resources, Commission on Life Sciences 2011) (32 ) and were approved by the Ethics Committee of the First Affiliated Hospital of Nanchang University. All experimental procedures were conducted and performed by experts who were blinded to the experiment conditions.
6
Establishing Orthotropic 4T1 Tumor Model
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Female BALB/c mice (18–2 g) and male Sprague–Dawley rats (180–220 g) were purchased from the Shanghai Laboratory Animal Center (Shanghai, China). Animal assays were conducted in accordance with the Guidelines for Animal Experimentation of Nanjing University of Chinese Medicine, and the protocol was approved by the institution’s Animal Ethics Committee (Nanjing, China. Certificate No.: SYXK-2018-0049).
4T1 cells were suspended and injected into the right inguinal mammary fat pad of the mice to establish an orthotropic 4T1 tumor-bearing mouse model [41 (link)]. The tumor volume (V) was assessed by measuring the length (a) and width (b) with calipers and then calculated using the following equation:
4T1 cells were suspended and injected into the right inguinal mammary fat pad of the mice to establish an orthotropic 4T1 tumor-bearing mouse model [41 (link)]. The tumor volume (V) was assessed by measuring the length (a) and width (b) with calipers and then calculated using the following equation:
7
Murine 4T1 Breast Cancer Cell Line Culture
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Murine 4T1 breast cancer cell line was obtained from the cell bank of the Chinese Academy of Sciences and was cultured in RPMI 1640 (Invitrogen, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 1% antibiotics (C100C5, New Cell &Molecular Biotech, Suzhou, China). The cells were maintained at 37 °C in a humidified incubator containing 5% CO2.
Female BALB/c mice (18–22 g) and female C57BL/6 mice (18–22 g) were purchased from the Shanghai Experimental Animal Center (Shanghai, China). OT-I mice (18–22 g) were purchased from Cyagen Biosciences (Shanghai, China). All the animals were maintained in a 12 h/12 h light/dark cycle with free access to food and water. All the animal experiments were approved by the Institutional Animal Care and Use Committee of the Shanghai Institute of Materia Medica, Chinese Academy of Sciences (2021-06-LYP-43).
Female BALB/c mice (18–22 g) and female C57BL/6 mice (18–22 g) were purchased from the Shanghai Experimental Animal Center (Shanghai, China). OT-I mice (18–22 g) were purchased from Cyagen Biosciences (Shanghai, China). All the animals were maintained in a 12 h/12 h light/dark cycle with free access to food and water. All the animal experiments were approved by the Institutional Animal Care and Use Committee of the Shanghai Institute of Materia Medica, Chinese Academy of Sciences (2021-06-LYP-43).
8
COS-7 Cell Culture and BALB/c Mouse Experiments
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COS-7 cells were ordered from ATCC and cultured in our laboratory.COS-7 cells originated from African green monkey kidney and it is a fibroblast-like cell line suitable for vector transfection [17 (link)]. Female BALB/c mice (4 to 6 weeks old) were obtained from the Shanghai Experimental Animal Center. All animal experiments were compliant with the local and governmental regulations.
9
Oral Infection of BALB/c Mice with C. albicans
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Female BALB/c mice were purchased from Shanghai Laboratory Animal Center (Shanghai, China). Mice that were 6–8 weeks old and weighed 16-20g were used. These mice were maintained in a pathogen-free animal facility at Institut Pasteur of Shanghai. Infections were performed under SPF conditions. Animals were inoculated by oral gavage with the indicated dose of C. albicans cells in 100μl PBS buffer.
10
Evaluating BMVE for Tumor Biodistribution
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Female BALB/c mice aged 4 to 6 weeks were obtained from the Shanghai Laboratory Animal Center. All mice were raised in an SPF-approved animal facility. All procedures were approved by the Animal Ethics Committee of Shanghai Jiao Tong University and carried out in accordance with the University’s Guidelines for Care and Use of Laboratory Animals. In total, 2 × 106 4T1 cells were injected into the mice’s flanks. Experiments were started when the mean volume reached 100 mm3. A total of 16 mice were used, and they were randomly divided into two groups of 8 mice each, named the control group and the experimental group. DiR dye or DiR-labeled BMVE was administered to each group of mice by tail vein or intratumoral injection, and fluorescence imaging was performed to observe the biodistribution of BMVE in the mice, as well as BMVE intratumoral injection to observe tumor treatment in vivo.
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