2ʹ,7ʹ-Dichlorofluorescin Diacetate (DCFDA), β-Endorphin, cetyl trimethyl ammonium bromide (CTAB), Toluene diisocyanate (TDI), Trolox, Vanadium chloride (III) and Xylenol orange were purchased from Sigma-Aldrich (USA). Bovine Serum Albumin (BSA), o-dianisidine dihydrochloride (ODD), methionine, Glutathione reduced (GSH), glutathione oxidised, nicotinamide adenine dinucleotide reduced (NADH), hydroxylamine hydrochloride and riboflavin were purchased from Sisco Research Laboratory (Mumbai, India). Sodium azide, 5, 5-Dithiobis-2-Nitrobenzoic acid (DTNB) and 1-Chloro-2,4-dinitrobenzene, (CDNB) were purchased from Loba chem. (India).
β endorphin
Manufactured by Merck Group
Sourced in United States, Japan
β-Endorphin is a laboratory reagent used for research purposes. It is a naturally occurring peptide produced in the human body that functions as a neurotransmitter and neuromodulator.
Automatically generated - may contain errors
Lab products found in correlation
Cetyltrimethylammonium bromide, by Merck Group (1 mentions)
Reduced glutathione, by Sisco Research Laboratories (1 mentions)
Reduced nicotinamide adenine dinucleotide, by Sisco Research Laboratories (1 mentions)
Trolox, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Sisco Research Laboratories (1 mentions)
Xylenol orange, by Merck Group (1 mentions)
Toluene diisocyanate, by Merck Group (1 mentions)
Methionine, by Sisco Research Laboratories (1 mentions)
Hydroxylamine hydrochloride, by Sisco Research Laboratories (1 mentions)
Riboflavin, by Sisco Research Laboratories (1 mentions)
Neuropeptide y, by Merck Group (1 mentions)
α melanocyte stimulating hormone, by Merck Group (1 mentions)
2 protocols using β endorphin
1
Biochemical Assays for Antioxidant and Enzyme Activity
2
Hypothalamic Peptide Binding Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The antiserum obtained (gC2 (1:30,000)) was incubated with 10 mmol of several hypothalamic peptides dissolved in 100 or 200 µl distilled water, or an equal volume of distilled water (control), for 1 h at RT and overnight at 4°C.
The pre-treated serum was then used for kisspeptin single-labeling immunohistochemistry as described above. The following peptides were used: rat- or human-type kisspeptin-10 and GnRH (Peptide Institute, Minoh, Osaka, Japan), NKB,
β-endorphin (ovine), Neuropeptide Y (NPY, ovine), α-melanocyte-stimulating hormone (Sigma-Aldrich, St Louis, MO, USA), Dyn (porcine), prolactin-releasing peptide-31 (bovine), prepro-RF-amide-related peptide (RFRP) (rat, 103-125),
pyroglutamylated-RF-amide peptide (rat, 13-26), and SP (human, 2-11) (Phoenix Pharmaceuticals Inc).
The anti-SP monoclonal antibody (1:2,000,000) was incubated with either SP (10 nmol), NKB (10 nmol), or 100 µl distilled water for 1 h at RT and overnight at 4°C, and subjected to SP immunohistochemistry using a similar protocol
as the kisspeptin single-labeling immunohistochemistry, except that the normal serum and the second antibody were substituted with normal horse serum and anti-mouse IgG, respectively.
The pre-treated serum was then used for kisspeptin single-labeling immunohistochemistry as described above. The following peptides were used: rat- or human-type kisspeptin-10 and GnRH (Peptide Institute, Minoh, Osaka, Japan), NKB,
β-endorphin (ovine), Neuropeptide Y (NPY, ovine), α-melanocyte-stimulating hormone (Sigma-Aldrich, St Louis, MO, USA), Dyn (porcine), prolactin-releasing peptide-31 (bovine), prepro-RF-amide-related peptide (RFRP) (rat, 103-125),
pyroglutamylated-RF-amide peptide (rat, 13-26), and SP (human, 2-11) (Phoenix Pharmaceuticals Inc).
The anti-SP monoclonal antibody (1:2,000,000) was incubated with either SP (10 nmol), NKB (10 nmol), or 100 µl distilled water for 1 h at RT and overnight at 4°C, and subjected to SP immunohistochemistry using a similar protocol
as the kisspeptin single-labeling immunohistochemistry, except that the normal serum and the second antibody were substituted with normal horse serum and anti-mouse IgG, respectively.
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