The expression levels of growth factors (41 human proteins) in fresh medium were analyzed using a RayBio C-Series Human Growth Factor Antibody Array C1 (RayBiotech, Norcross, GA) according to the manufacturer's instructions. Briefly, 1 ml of the cell-free culture supernatant of each group from day 7 was incubated with the membrane in the well of the incubation tray at 4 °C overnight. Following repeated washing, the membrane was incubated with a biotinylated antibody cocktail for 2 h at room temperature and then with horseradish peroxidase–conjugated streptavidin for 2 h at room temperature. Then the membrane was visualized with enhanced chemiluminescence (Amersham, Arlington Heights, IL). Relative protein levels were determined by densitometry and normalized to positive controls using ImageJ software.
Raybio c series human growth factor antibody array c1
Manufactured by RayBiotech
Sourced in United States
The RayBio C-Series Human Growth Factor Antibody Array C1 is a lab equipment product designed for the simultaneous detection and quantification of multiple human growth factor proteins in a single experiment. It provides a comprehensive analysis of growth factor levels in biological samples.
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4 protocols using raybio c series human growth factor antibody array c1
1
Growth Factor Protein Expression Analysis
2
Growth Factor Profiling in VCaP Cells
3
Cytokine and Growth Factor Analysis
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An analysis of cytokines in conditioned media was performed using the Proteome Profiler Human Cytokine Array Panel A (R&D Systems) according to the manufacturer’s instructions. A growth factor analyses was performed using the Ray Bio C-Series Human Growth Factor Antibody Array C1 (Ray Biotech, Norcross, GA, USA). Colorimetric analysis using a luminescent image analyzer (Image Quant LAS 4000 mini) was used to quantify the intensity of each membrane dot, which allowed for the assessment of growth factor content in the UCMSC-CM. The conditioned media was used after 20-fold concentration.
4
Growth Factor Profiling in IVD Culture
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A commercially available array of 42 growth factor proteins (RayBio® C-Series Human Growth Factor Antibody Array C1, #AAH-GF-1-4, RayBiotech, Inc. 3607 Parkway Lane, Suite) was used to evaluate the relative levels of growth factors production in the IVD culture media of both cavity and C + hMSCs at day 21. A pool of culture media collected from 6 independent experiments was prepared for this assay, and 1 mL of the prepared pool was used. The array was performed by following the manufacturer’s instructions. Data shown represents 5 min exposure in Chemidoc XRSþ (BioRad). Results were generated by quantifying the mean spot pixel density from the array using image software analyses (ImageLab 4.1; BioRad). The densities of signals obtained were normalized with the background and results presented as a mean of two spots per growth factor.
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