The hydrogels were washed 2 × 5 min in 0.1 M Sorensen´s buffer (pH 7.4) (S/3760/60 Fisher Scientific and S/4520/60, Fisher Scientific) and fixed in approximately 10 times the sample volume of “SEM fix” (0.1 M Sorensen´s phosphate buffer pH 7.4, 2% formaldehyde (P001, TAAB) and 2% glutaraldehyde (18427, Ted Pella Inc)) at room temperature for 20 min, followed by washing 2 × 5 min in 0.1 M Sorensen´s buffer pH 7.4. Samples were then dehydrated in a graded series of ethanol (50%, 70%, 80%, 90%, and twice in 100%) and subsequently critical point dried before being mounted and sputter coated with 5–10 nm Pt/Pd (80:20) (MN70-PP5708, Micro to Nano) on a turbomolecular pump coater (Q150T ES, Quorum). As cryogels were already freeze dried, they were directly mounted and coated with 5–10 nm Pt/Pd (80:20) and all samples were examined in a JSM-7800F FEG-SEM (Jeol).
Jsm 7800f feg sem
Manufactured by JEOL
Sourced in Japan
The JSM-7800F FEG-SEM is a field emission scanning electron microscope (FEG-SEM) manufactured by JEOL. It provides high-resolution imaging capabilities with a wide range of magnification levels. The instrument utilizes a field emission gun as the electron source, which allows for improved spatial resolution and reduced chromatic aberration compared to traditional thermionic electron guns.
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Lab products found in correlation
9 protocols using jsm 7800f feg sem
1
Hydrogel Preparation for SEM Analysis
2
Fixation and Imaging of Repopulated Lung Scaffolds
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The repopulated lung scaffolds were fixed in buffer containing 0.1 M Sorensen’s phosphate buffer pH 7.4, 1.5% formaldehyde and 1.5% glutaraldehyde at RT for 2 h. Afterwards, the samples were washed twice in 0.1 M Sorensen ’s phosphate buffer, pH 7.4, followed by dehydration in ethanol. The samples were mounted and examined in a Jeol JSM-7800F FEG-SEM at Lund University Bioimaging Centre (LBIC).
3
Lung Tissue Preparation for SEM Analysis
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Lung slices for scanning electron microscopy (SEM) were fixed in buffer containing 0.1 M Sorensen´s phosphate buffer pH 7.4, 1.5% formaldehyde and 1.5% glutaraldehyde at RT for 2 hours. After fixation, the samples were washed twice in 0.1 M Sorensen´s phosphate buffer pH 7.4, before being dehydrated in a graded series of ethanol (50%, 70%, 80%, 90% and twice in 100%). Samples were critical point dried before being mounted and examined in a Jeol JSM-7800F FEG-SEM at Lund University Bioimaging Center.
4
Scanning Electron Microscopy Sample Preparation
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The samples were viewed with scanning electron microscopy and prepared according to local standards and protocol. Briefly, BEAS-2B was fixed in approximately 10 times the sample volume of fixation solution containing 0.1M Sorensen’s phosphate buffer pH 7.4, 1.5% formaldehyde and 1.5% glutaraldehyde at room temperature for 20 min. After fixation, the samples were washed twice in 0.1M Sorensen´s buffer pH 7.4 to remove excess fixative. Samples were then dehydrated in a graded series of ethanol (50%, 70%, 80%, 90% and twice in 100%) and subsequently, critical point dried before being mounted and examined in a Jeol JSM-7800F FEG-SEM.
5
Fixation and Dehydration Protocol for BEAS-2B Cells
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BEAS-2B were fixed in approximately ten times the sample volume of fixation solution containing 0.1 M Sorensen´s phosphate buffer pH 7.4, 1.5% formaldehyde and 1.5% glutaraldehyde at room temperature for 20 min. After fixation the samples were washed twice in 0.1 M Sorensen´s buffer pH 7.4 to remove excess fixative. Samples were then dehydrated in a graded series of ethanol (50%, 70%, 80%, 90% and twice in 100%) and subsequently critical point dried before being mounted and examined in a Jeol JSM-7800 F FEG-SEM.
6
SEM Preparation of Lung Tissues
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Lung tissues were fixed overnight at 4 °C in “SEM fix” (0.1 M Sorenson’s phosphate buffer at pH 7.4, 2% formaldehyde and 2% glutaraldehyde). After fixation, samples were washed twice in 0.1 M Sorenson’s buffer (pH 7.4). Washed samples were dehydrated in a graded series of ethanol (50%, 70%, 80%, 90%, and twice in 100%) and critical point dried. Samples were mounted on 12.5-mm aluminum stubs and sputtered with 10 nm Au/Pd (80/20) in a Quorum Q150T ES turbo-pumped sputter coater. Finally, prepared samples were examined in a Jeol JSM-7800F FEG-SEM (JEOL, Japan).
7
SEM Sample Preparation Protocol
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For SEM imaging, 4.0 × 4.0 mm samples were cut from either undamaged tissue or from the center of the wounds, prior to or after treatments, using a scalpel. The samples were first washed once in 0.1M Sorensen’s phosphate buffer pH 7.4, then fixed in approximately 2 mL of “SEM fix” (0.1M Sorenson’s phosphate buffer pH 7.4, 2% formaldehyde and 2% glutaraldehyde) at room temperature for 20 min. After fixation, samples were washed once in 0.1M Sorenson’s buffer pH 7.4. Fixed tissue samples were then dehydrated in a graded series of ethanol (50%, 70%, 80%, 90% and twice in 100%). Dehydrated tissue samples were then critical point dried (Quorum, Laughton, United Kingdom) and mounted on 12.5 mm aluminum stubs. Finally, mounted samples were sputtered with 10 nm gold/palladium (80/20) in a Quorum Q150T ES turbo pumped sputter coater (Quorum, Laughton, UK). Samples were then scanned and imaged in a Jeol JSM-7800F FEG-SEM (Tokyo, Japan).
8
SEM Preparation of Lung Tissues
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Lung tissues were fixed overnight at 4 °C in “SEM fix” (0.1 M Sorenson’s phosphate buffer at pH 7.4, 2% formaldehyde and 2% glutaraldehyde). After fixation, samples were washed twice in 0.1 M Sorenson’s buffer (pH 7.4). Washed samples were dehydrated in a graded series of ethanol (50%, 70%, 80%, 90%, and twice in 100%) and critical point dried. Samples were mounted on 12.5-mm aluminum stubs and sputtered with 10 nm Au/Pd (80/20) in a Quorum Q150T ES turbo-pumped sputter coater. Finally, prepared samples were examined in a Jeol JSM-7800F FEG-SEM (JEOL, Japan).
9
SEM Preparation of Lung Tissues
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Lung tissues were fixed overnight at 4 °C in “SEM fix” (0.1 M Sorenson’s phosphate buffer at pH 7.4, 2% formaldehyde and 2% glutaraldehyde). After fixation, samples were washed twice in 0.1 M Sorenson’s buffer (pH 7.4). Washed samples were dehydrated in a graded series of ethanol (50%, 70%, 80%, 90%, and twice in 100%) and critical point dried. Samples were mounted on 12.5-mm aluminum stubs and sputtered with 10 nm Au/Pd (80/20) in a Quorum Q150T ES turbo-pumped sputter coater. Finally, prepared samples were examined in a Jeol JSM-7800F FEG-SEM (JEOL, Japan).
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