All slides were observed under the compound light microscope Carl Zeiss Axio Lab.A1 (Carl Zeiss AG, Oberkochen, Germany). Measurements and pictures were taken using ZEN lite software with the support of ZEISS Axiocam ERc5s digital camera (Carl Zeiss AG, Oberkochen, Germany). Pencil drawings were obtained using an Olympus BX51 DIC Microscope equipped with a digital camera and a drawing tube. Illustrations were made using Illustrator CS3 based on pencil drawing, microphotographs, and SEM pics. Identification was based on the diagnostic key of Eisenback (1985) , Hewlett & Tarjan (1983) , Jepson (1987) , Karssen (2002) , and Kazachenko & Mukhina (2013) . Furthermore, a comparison with other recently described species was also accomplished (Humphreys et al., 2014 ; Tao et al., 2017 (link); Trinh et al., 2018 (link)).
Axiocam erc5s digital camera
Manufactured by Zeiss
Sourced in Germany
The Axiocam ERc5s is a digital camera designed for use with Zeiss microscopes. It features a 5-megapixel CMOS sensor and provides high-quality image capture capabilities for a range of microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
Zen 2, by Zeiss (2 mentions)
Axio lab a1 microscope, by Zeiss (2 mentions)
Primo star microscope, by Zeiss (2 mentions)
Axio lab 1 microscope, by Zeiss (2 mentions)
Facscanto 2, by BD (2 mentions)
Axion software 4, by Zeiss (2 mentions)
Fitc conjugated mouse anti human cd41 moab, by Agilent Technologies (2 mentions)
Pe conjugated mouse anti human cd42b moab, by Agilent Technologies (2 mentions)
Pe conjugated mouse anti human gpa moab, by Agilent Technologies (2 mentions)
Ax10scopea1 microscope, by Zeiss (2 mentions)
Bx51 dic microscope, by Olympus (1 mentions)
Horseradish peroxidase conjugated anti mouse secondary antibody, by Agilent Technologies (1 mentions)
Proteinase k, by Agilent Technologies (1 mentions)
Bx53 light microscope, by Olympus (1 mentions)
Uc90 camera, by Olympus (1 mentions)
Supra 40vp, by Zeiss (1 mentions)
Lsm710 system, by Zeiss (1 mentions)
Axiocam mrm, by Zeiss (1 mentions)
Axioimager system, by Zeiss (1 mentions)
Axiocam mrc5, by Zeiss (1 mentions)
21 protocols using axiocam erc5s digital camera
1
Nematode Identification Microscopy Protocol
2
Gastrointestinal Morphometry in Lambs
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On the 50th day, after weighing, lambs were anesthetized with sodium pentobarbital. Post-slaughter, we promptly extracted the rumen and small intestine, rinsing them with PBS buffer. We then measured the weight of each stomach compartment and recorded the length of each intestinal segment. The rumen, reticulum, omasum, and abomasum were separated. Rumen tissue samples, approximately 2 cm × 2 cm, were cut and immersed in 4% formaldehyde for histological analysis. Similar procedures were followed for the duodenum, jejunum, and ileum, with 10 cm sections sampled for histomorphological assessments. The Axio Lab. A1 microscope (Carl Zeiss, Jena, Germany) equipped with a Zeiss Axiocam ERc 5s digital camera was used for histological assessment. Images were analyzed and captured using ZEN 2.3 software (Carl Zeiss Microscopy GmbH, Oberkochen, Germany, 2011). Villus height (VH) and crypt depth (CD) were measured, and the V/C ratio (Villus height/Crypt depth, V/C) was calculated based on these measurements. The stomach coefficient was equal to stomach weight (g)/antemortem live weight (g) × 100%. The intestinal coefficient was equal to intestinal length (m)/antemortem live weight (kg).
3
Quantifying Cytokeratin Expression in 3D Tissues
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Paraffin‐embedded 3‐D tissues were sectioned at a thickness of 6 µm; the sections were stained with hematoxylin and eosin. For immunohistochemistry, sections were deparaffinized, and incubated for 5 min at 20°C with proteinase K (Dako, Glostrup, Denmark) diluted 1:1000 in phosphate‐buffered saline [PBS (−)] followed by 3% hydrogen peroxide. After washing, sections were incubated in 1% bovine serum albumin for at least 20 min at room temperature. A mouse monoclonal antibody against human cytokeratin (CK) (Dako) diluted 1:500 was added overnight at 4°C, followed by incubation for 30 min at room temperature with horseradish peroxidase‐conjugated antimouse secondary antibody (Dako). After washing with PBS (−), staining with diaminobenzidine (Dako), and nuclear staining with Mayer's hematoxylin for 30 s, sections were washed and imaged under a light microscope (400× magnification) with an AxioCamERc5s digital camera (Carl Zeiss, Oberkochen, Germany). Three randomly selected views were imaged for each of the three specimens. The average of number of CK‐positive cells that reached the membrane at the bottom of the 3‐D tissue was determined.
4
Histological Analysis of Gonadal Samples
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Gonadal samples were fixed in 10% formalin, processed, and embedded into paraffin blocks. The 3 µm serial sections were stained with hematoxylin and eosin. The slides were analyzed on an Axio Lab.A1 microscope (Carl Zeiss Microscopy, Jena, Germany) equipped with an Axiocam ERc5s digital camera (Carl Zeiss Microscopy, Jena, Germany) or on Olympus BX53 light microscope coupled with an Olympus UC90 camera.
5
SEM Imaging of Transgenic Arabidopsis Roots
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SEM was done with a SUPRA 40VP (Carl Zeiss MicroImaging) equipped with a K1250X Cryogenic SEM Preparation System (EMITECH), a CPD 030 critical point dryer (Bal-Tec) and a SC 7600 sputter coater (Polaron) at the on-campus microscopy core facility Zentrale Mikroskopie (CeMic) of the Max Planck Institute for Plant Breeding Research, Cologne. Light and confocal laser scanning microscopy was performed with an LSM710 system (Carl Zeiss MicroImaging). K2:GFP fluorescence was observed in root tips of plants that were aseptically grown for 2 weeks under long-day conditions at either constitutively restrictive or permissive temperatures on 0.5 MS medium containing 50 mg l−1 kanamycin sulfate. Photographs of in vitro cultures and histological stainings were taken with a stereo microscope (Stemi 2000-C) equipped with a Zeiss CL 6000 LED illumination unit, and a video adapter 60 C including an AxioCam ERc 5s digital camera (all from Carl Zeiss MicroImaging).
6
Visualizing Plant Trichome Structures
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Photographs of in vitro cultures and histological staining were taken with a stereomicroscope (Stemi 2000-C) equipped with a Zeiss CL 6000 LED illumination unit, and a video adapter 60 C including an AxioCam ERc 5s digital camera (all from Carl Zeiss MicroImaging). Trichome visualization through polarized light microscopy was carried out as described previously (Gudesblat et al., 2012 (link); Pomeranz et al., 2013 (link)) with the only change that destaining was achieved with a saturated chloral hydrate solution (dissolve 1 kg in 400 mL of water). Trichomes were visualized using a Nikon AZ100 zoom microscope equipped with a Nikon DS-Ri2 color camera. Microscopy images of trichomes and DAPI-stained nuclei were obtained using the AxioImager system (Zeiss) equipped with two cameras (AxioCam MRm and AxioCam MRc5).
7
Fluorescence Microscopy of Fungal Morphogenesis
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Fluorescence microscopy was performed using an AxioImager M.1 microscope (Zeiss) equipped with a CoolSnap HQ camera (Roper Scientific) and a SpectraX LED lamp (Lumencor). Images were captured and edited with MetaMorph (Universal Imaging). For localization of PRO34, strains were grown on BMM-covered slides [31 (link)] for two days. Mitochondria were stained with 100 µM MitoTracker orange CMTMRos (Life Technologies, Darmstadt, Germany). GFP and MitoTracker fluorescence was analyzed using Chroma filter sets (Chroma Technology Corp.) 41017 (HQ470/40, HQ525/50, Q495lp) and 49008 (HQ560/40, ET630/75m, T585lp), respectively.
Hyphal fusion was observed after two days of growth on MMS with cellophane as described using the AxioImager M.1 microscope (Zeiss) [27 (link)].
Perithecia formation was assayed on BMM plates after seven days of growth using a Stemi 2000-C stereomicroscope (Zeiss) equipped with an AxioCamERc5s digital camera (Zeiss) and AxioVision software (Zeiss). Ascospore formation was assayed after ten days of growth on BMM plates. Perithecia were cracked open and ascus rosettes were imaged on slides using the AxioImager M.1 microscope (Zeiss). Images were processed with Adobe CS4 and CS6 (Adobe Corp.).
Hyphal fusion was observed after two days of growth on MMS with cellophane as described using the AxioImager M.1 microscope (Zeiss) [27 (link)].
Perithecia formation was assayed on BMM plates after seven days of growth using a Stemi 2000-C stereomicroscope (Zeiss) equipped with an AxioCamERc5s digital camera (Zeiss) and AxioVision software (Zeiss). Ascospore formation was assayed after ten days of growth on BMM plates. Perithecia were cracked open and ascus rosettes were imaged on slides using the AxioImager M.1 microscope (Zeiss). Images were processed with Adobe CS4 and CS6 (Adobe Corp.).
8
Vascular Bundle and Adventitious Root Analysis
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The images of in situ and Toluidine Blue-O-stained samples were acquired with a Zeiss Axiocam ERC 5 s digital camera on a Zeiss compound light microscope (Carl Zeiss Microscopy LLC, Thornwood, NY). To confirm gene expression patterns, each in situ experiment was repeated at least two times using different biological samples. Photoshop CC (Adobe Inc., Mountain View, CA) was used for counting cells in the vascular bundle and numbers of adventitious roots from images.
9
Nematode Extraction and Characterization
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Nematodes were extracted from soil samples using modified Baermann tray method (Whitehead and Hemming, 1965 ). For morphological characterizations, permanent slides of nematodes were observed through the Carl Zeiss Axio Lab.A1 light microscope. Measurements and pictures were taken using a ZEN lite software on ZEISS Axiocam ERc5s digital camera (Nguyen et al., 2017 (link)).
For molecular studies, Primers D2A (5′-ACAAGTACCGTGGGGAAA GTTG-3′) and D3B (5′-TCGGAAGGAACCAGCTAC TA-3′) were used to amplify D2D3 of 28S rDNA region (Nguyen et al., 2017 (link)). Obtained sequence was used for a Blast search in GenBank (Altschul et al., 1997 (link)). The data set was analyzed using maximum likelihood (ML) method in MEGA 6 program with 1,000 bootstrap replications. The best fit model of DNA evolution was obtained using the Model test in MEGA 6 (Nguyen et al., 2017 (link)).
For molecular studies, Primers D2A (5′-ACAAGTACCGTGGGGAAA GTTG-3′) and D3B (5′-TCGGAAGGAACCAGCTAC TA-3′) were used to amplify D2D3 of 28S rDNA region (Nguyen et al., 2017 (link)). Obtained sequence was used for a Blast search in GenBank (Altschul et al., 1997 (link)). The data set was analyzed using maximum likelihood (ML) method in MEGA 6 program with 1,000 bootstrap replications. The best fit model of DNA evolution was obtained using the Model test in MEGA 6 (Nguyen et al., 2017 (link)).
10
Histological Evaluation of Implantation Site
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The animals were humanely euthanized after one week and one month post-surgery by administering an overdose of ketamine (100 mg/kg). Tissue samples from the implantation site, liver, and kidneys were fixed in a 10% neutral buffered formalin solution (pH 7.2) for 24 h. For histological analysis, 5 μm thick sections were prepared from paraffin-embedded tissue blocks and mounted on SuperFrost microscopic slides (Thermo Fisher Scientific; Waltham, MA, USA). The slides underwent standard deparaffinization using xylene and rehydration with a series of decreasing ethanol concentrations. Subsequently, the samples were stained with hematoxylin and eosin using conventional methods to enable visual evaluation of the histological specimens. All examinations were performed using a Carl Zeiss Primo Star microscope equipped with a Zeiss AxioCam ERc 5s digital camera and ZEN 2 (blue edition) software (Oberkochen, Germany). Morphometric parameters were assessed in 10 fields of view at ×100 magnification by two independent observers.
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