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Peroxidase coupled secondary antibodies

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Peroxidase-coupled secondary antibodies are protein-based reagents designed for use in immunoassays. They are used to detect and amplify the signal from primary antibodies that have bound to target antigens. The peroxidase enzyme catalyzes a colorimetric reaction, allowing for the visualization and quantification of the target analyte.

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Lab products found in correlation

Nitrocellulose, by Thermo Fisher Scientific (3 mentions) West dura chemiluminescence substrate, by Thermo Fisher Scientific (3 mentions)

3 protocols using peroxidase coupled secondary antibodies

1

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were resolved by SDS-PAGE. The proteins were transferred to nitrocellulose (Thermo Fisher) and immunodetected using appropriate primary and peroxidase-coupled secondary antibodies (Genesee Scientific). Proteins were visualized by West Pico and West Dura Chemiluminescence Substrate (Thermo Fisher).
Boice A.G., Lopez K.E., Pandita R.K., Parsons M.J., Charendoff C.I., Charaka V., Carisey A.F., Pandita T.K, & Bouchier-Hayes L. (2021). Caspase-2 regulates S-phase cell cycle events to protect from DNA damage accumulation independent of apoptosis. Oncogene, 41(2), 204-219.
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2

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were resolved by SDS-PAGE. The proteins were transferred to nitrocellulose (Thermo Fisher) and immunodetected using appropriate primary and peroxidase-coupled secondary antibodies (Genesee Scientific). Proteins were visualized by West Pico and West Dura Chemiluminescence Substrate (Thermo Fisher).
Boice A.G., Lopez K.E., Pandita R.K., Parsons M.J., Charendoff C.I., Charaka V., Carisey A.F., Pandita T.K, & Bouchier-Hayes L. (2021). Caspase-2 regulates S-phase cell cycle events to protect from DNA damage accumulation independent of apoptosis. Oncogene, 41(2), 204-219.
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3

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were resolved by SDS-PAGE. The proteins were transferred to nitrocellulose (Thermo Fisher) and immunodetected using appropriate primary and peroxidase-coupled secondary antibodies (Genesee Scientific). Proteins were visualized by West Pico and West Dura chemiluminescence Substrate (Thermo Fisher).
Boice A., Pandita R.K., López K.E.R., Parsons M.J., Charendoff C.I., Charaka V., Carisey A.F., Pandita T.K., & Bouchier‐Hayes L. (2021). Caspase-2 regulates S-phase cell cycle events to protect from DNA damage accumulation independent of apoptosis.
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