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Alexafluor 647 anti b220

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AlexaFluor 647 anti-B220 is a fluorescently-labeled antibody that binds to the B220 (CD45R) protein, which is expressed on the surface of B lymphocytes. This product can be used for flow cytometry applications to identify and quantify B cells in biological samples.

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Lab products found in correlation

Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Biotinylated anti igd, by BioLegend (2 mentions) Alexa fluor 488 anti cd45, by BioLegend (2 mentions) Streptavidin alexafluor 568, by Thermo Fisher Scientific (2 mentions) Biotinylated pna, by Vector Laboratories (2 mentions) Fv1000 confocal system, by Olympus (2 mentions)

Close spelling variants of this product

Anti-B220

2 protocols using alexafluor 647 anti b220

1

Multicolor Immunofluorescent Staining Protocol

Cited 1 time
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The protocol for immunohistochemical staining was as described previously30 (link). Staining reagents included biotinylated PNA (Vector), AlexaFluor 647 anti-B220, biotinylated anti-IgD, AlexaFluor 488 anti-CD45.2 (Biolegend), and streptavidin AlexaFluor 568 (Invitrogen). All stained slides were mounted with ProlongGold antifade reagents (Invitrogen) and examined with an Olympus FV1000 confocal system.
Liu X., Chen X., Zhong B., Wang A., Wang X., Chu F., Nurieva R.I., Yan X., Chen P., van der Flier L.G., Nakatsukasa H., Neelapu S.S., Chen W., Clevers H., Tian Q., Qi H., Wei L, & Dong C. (2014). Transcription factor Achaete-Scute homologue 2 initiates T follicular helper cell development. Nature, 507(7493), 513-518.
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2

Multicolor Immunofluorescent Staining Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The protocol for immunohistochemical staining was as described previously30 (link). Staining reagents included biotinylated PNA (Vector), AlexaFluor 647 anti-B220, biotinylated anti-IgD, AlexaFluor 488 anti-CD45.2 (Biolegend), and streptavidin AlexaFluor 568 (Invitrogen). All stained slides were mounted with ProlongGold antifade reagents (Invitrogen) and examined with an Olympus FV1000 confocal system.
Liu X., Chen X., Zhong B., Wang A., Wang X., Chu F., Nurieva R.I., Yan X., Chen P., van der Flier L.G., Nakatsukasa H., Neelapu S.S., Chen W., Clevers H., Tian Q., Qi H., Wei L, & Dong C. (2014). Transcription factor Achaete-Scute homologue 2 initiates T follicular helper cell development. Nature, 507(7493), 513-518.
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