Sc 21731

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

sc-21731 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research applications. The core function of this product is to provide a specific laboratory tool or component, but a detailed description cannot be provided while maintaining an unbiased and factual approach without making interpretations or extrapolations.

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Lab products found in correlation

2 protocols using sc 21731

Comprehensive Protein Expression Analysis

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Western blot analysis was carried out on whole cell lysates. Antibodies against Tim-3 (Ab241332), NF-κB (p65) (Ab16502), p-NF-κB (p-p65) (Ab194726) and vascular endothelial growth factor (VEGF) A (Ab9570) were purchased from (Abcam Cambridge, UK). GAPDH (sc-47724), p-β-catenin (sc-16743-R), cyclin D1 (CCND1) (sc-8396), C-Myc (sc-70465), matrix metalloproteinase-1 (MMP1) (sc-21731), TWIST (sc-6269), zona occludens (ZO)-1 (sc-10804), ZO-2 (sc-11448), occludin (sc-133256) and VEGFB (sc-13083) antibodies were obtained from Santa Cruz (Insight Biotechnology Limited, Middlesex UK). E-Cadherin (AF748) and VEGFD (MAB286) antibodies were purchased from R&D Systems (Abingdon, Oxfordshire, UK). STAT3 (S5933), p-STAT3 (SAB4504541), interleukin (IL)-6 (17901) and β-catenin (C2206) antibodies were purchased from Sigma-Aldrich (Gillingham, Dorset, UK). Anti-mouse (A5278), anti-rabbit (A0545) and anti-goat (A8919) secondary antibodies were obtained from Sigma-Aldrich (Gillingham, Dorset, UK).

Cong Y., Cui Y., Zhu S., Cao J., Zou H., Martin T.A., Qiao G., Jiang W, & Yu Z. (2020). Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells. Chinese Journal of Cancer Research, 32(5), 564-579.

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Immunohistochemical Profiling of Raft Cultures

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We fixed raft cultures in 4% neutral buffered formalin and then embedded in paraffin for sectioning and hematoxylin and eosin (H&E) staining. Primary rabbit monoclonal antibodies to keratin 14 (ab51054, Abcam) and Ki-67 (ab16667, Abcam) and primary mouse monoclonal antibodies to K18 (ab7797, Abcam), pAkt1 (sc-293125, Santa Cruz) and MMP-1 (sc-21731, Santa Cruz) were used for immunohistochemistry on unstained sections. Secondary donkey anti-mouse antibody (ab150105, Abcam) with Alexa 488 tag and secondary goat anti-rabbit antibody (ab150080, Abcam) with an Alexa 594 tag were used for visualization. Negative controls consisted of irrelevant primary antibodies including rabbit monoclonal isotype control (ab172730, Abcam) and mouse monoclonal isotype control (ab170190, Abcam). The gain and exposure time of the fluorescence microscope were adjusted to show positively stained cells while negative control cells had no staining. Staining percentage was calculated by dividing the positively stained cell number by the total cell number. Staining intensity was measured by ImageJ and the negative control was used to subtract background.

Deng H., Hillpot E., Mondal S., Khurana K.K, & Woodworth C.D. (2018). HPV16-Immortalized Cells from Human Transformation Zone and Endocervix are More Dysplastic than Ectocervical Cells in Organotypic Culture. Scientific Reports, 8, 15402.

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