Glycol methacrylate

Manufactured by Polysciences

Sourced in United Kingdom, United States

Glycol methacrylate is a clear, colorless liquid monomer used in the preparation of various polymeric materials. It is commonly utilized as a resin component in the formulation of embedding media for histological and biological sample preparation.

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Lab products found in correlation

Envision flex kit, by Agilent Technologies (2 mentions) Mouse monoclonal, by BD (1 mentions) Isotype control, by Agilent Technologies (1 mentions) Ab24070, by Abcam (1 mentions) M7254, by Agilent Technologies (1 mentions) M0752, by Agilent Technologies (1 mentions) M7103, by Abcam (1 mentions) M0821, by Agilent Technologies (1 mentions) Embed 812, by Electron Microscopy Sciences (1 mentions) Cm3050, by Leica camera (1 mentions) 80i microscope, by Nikon (1 mentions)

6 protocols using glycol methacrylate

Characterizing Scaffold Pore Structure

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The scaffolds were sectioned, embedded in glycol methacrylate (Polysciences, Warrington, PA, USA), and stained with toluidine blue. The stained images were used to calculate pore size and porosity of the scaffolds using pore topology analyzer software (MATLAB, Natick, MA, USA).

Wei P., Xu Y., Gu Y., Yao Q., Li J, & Wang L. (2020). IGF-1-releasing PLGA nanoparticles modified 3D printed PCL scaffolds for cartilage tissue engineering. Drug Delivery, 27(1), 1106-1114.

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Quantifying PP5 Expression in ASM

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Mucosal biopsy specimens were processed into glycol methacrylate (Polysciences, Northampton, UK). Three-micrometer sections were stained with antibody against human PP5 (mouse monoclonal, 2μg/ml, BD Bioscience), α-smooth muscle alpha actin (Millipore UK) or isotype control (Dako, UK). The EnVision FLEX kit (Dako, UK) was used for the staining of the sections. The quantitative assessment of PP5 staining in the ASM bundle was performed using a thresholding technique based on the 3,3’-Diaminobenzidine (DAB) staining that was evaluated using the Image J software as described in our previous study (26 (link)).

Chachi L., Abbassian M., Gavrila A., Alzahrani A., Tliba O., Bradding P., Wardlaw A.J., Brightling C, & Amrani Y. (2016). Protein phosphatase 5 mediates corticosteroid insensitivity in airway smooth muscle in patients with severe asthma. Allergy, 72(1), 126-136.

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Immunohistochemical Profiling of Airway Biopsies

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Mucosal biopsy specimens were processed into glycol methacrylate (Polysciences, Northampton, United Kingdom). Two-micrometer sections were stained with antibodies: DP2/CRTH2 (rabbit polyclonal against sequence CAASPQTGPLNRALSSTSS, 1 μg/mL; AstraZeneca, London, United Kingdom) with staining confirmed by an alternative antibody to DP2/CRTH2 OPA1-15328 (5 μg/mL; Thermo Fisher Scientific, Leicestershire, United Kingdom), mast cell tryptase (IR640; Dako, Cambridge, United Kingdom), CD3 (M7254, 5 μg/mL; Dako), major basic protein (MON6008, 1.3 μg/mL; Monosan, Newmarket, United Kingdom), neutrophil elastase (M0752, 0.02 μg/mL; Dako), CD4 (M7310, 5 μg/mL), CD8 (M7103, 1 μg/mL), MUC5AC (Ab24070, 1 μg/mL; Abcam, Cambridge, United Kingdom), pancytokeratin (M0821, 1 μg/mL; Dako), involucrin (Ab68, 0.75 μg/mL; Abcam), or isotype controls (Dako). The EnVision FLEX kit (Dako) was used. Colocalization was undertaken with sequential sections, as described previously.²¹ (link) Positively stained nucleated cells were enumerated per square millimeter of submucosal area, per 10 mm² of total epithelial area, or per millimeter of ALI culture length by a blinded observer. Grading criteria were derived for histology of biopsy specimens and area of involucrin-positive staining. Grading was carried out on 2 separate occasions by a blinded observer.

Stinson S.E., Amrani Y, & Brightling C.E. (2015). D prostanoid receptor 2 (chemoattractant receptor–homologous molecule expressed on TH2 cells) protein expression in asthmatic patients and its effects on bronchial epithelial cells. The Journal of Allergy and Clinical Immunology, 135(2), 395-406.e7.

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Zebrafish Synchrotron MicroCT Imaging Protocol

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Zebrafish specimens were anesthetized in 10x Finquel (MS-222 or tricaine) solution (Argent Chemical Laboratories (Redmond, WA) buffered in 1% Phosphate-buffered saline (PBS), and fixed in 10% Neutral Buffered Formalin (NBF) (Fisher Scientific, Allentown, PA). Samples were then stained with Uranium Acetate and Osmium Tetroxide (UaOs) (1% Ua and 1% Os) or 0.3% Phosphotungstic Acid (PTA) for 24 hours, infiltrated and embedded in Glycol Methacrylate (Polysciences, Inc., Warrington, PA) or EMBed-812 (Electron Microscopy Sciences, Hatfield, PA) in kapton tubing (Small Parts, Inc., Logansport, IN). Zebrafish were maintained under standard laboratory conditions and staged according to ZFIN13. All procedures on live animals were approved by the Institutional Animal Care and Use Committee (IACUC) at the Pennsylvania State University.
2.2. Synchrotron MicroCT Imaging was performed at the 2-BM beamline at the Advanced Photon Source (APS) of Argonne National Lab. The synchrotron radiations are an unfocused beam of 25 mm (horizontal) by 4 mm (vertical) with energy resolution (ΔE/E) of 1×10⁻² , flux (photons/sec) of 1×10¹² @17 keV, and energy range of 0.5–33 keV which can be tuned using a double multilayer monochromator. Diagrams and detailed description of synchrotron microCT imaging can be found elsewhere^{34 ,35}.

Xin X., Clark D., Ang K.C., van Rossum D.B., Copper J., Xiao X., La Riviere P.J, & Cheng K.C. (2017). Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections. Proceedings of SPIE--the International Society for Optical Engineering, 10060, 100601I.

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Murine Retinal Morphological Analysis

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Morphological analysis of murine retinas was performed as previously described (Hadziahmetovic et al., 2011 (link)). Briefly, mice were aged to 16 months and enucleated eyes were fixed overnight in 2% PFA/2% glutaraldehyde. Eyes were embedded in glycol methacrylate (Polysciences), cut into 3 μm sections using a microtome (Leica CM3050S), and stained with Toluidine Blue. Sections were imaged using the Nikon Elements software. Images were captured using a Nikon 80i microscope with a 40× objective, total 400× magnification and 0.75 aperture. The DS-Fi2 camera was used with the Nikon Elements software. Image intensity levels were adjusted uniformly across all photos within each experiment using Adobe Photoshop.

Zhang K.R., Jankowski C.S., Marshall R., Nair R., Más Gómez N., Alnemri A., Liu Y., Erler E., Ferrante J., Song Y., Bell B.A., Baumann B.H., Sterling J., Anderson B., Foshe S., Roof J., Fazelinia H., Spruce L.A., Chuang J.Z., Sung C.H., Dhingra A., Boesze-Battaglia K., Chavali V.R., Rabinowitz J.D., Mitchell C.H, & Dunaief J.L. (2023). Oxidative stress induces lysosomal membrane permeabilization and ceramide accumulation in retinal pigment epithelial cells. Disease Models & Mechanisms, 16(7), dmm050066.

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Mechanical Properties of nHAC/PLGA Scaffolds

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The effects of the added nanospheres on the mechanical behavior of scaffolds were evaluated and compared with unloaded nHAC scaffolds. Morphological characteristics of the surfaces and cross-sections obtained from the nHAC/PLGA composite scaffolds were observed using SEM. To quantitatively analyze the nHAC/PLGA composite support structure, the scaffold was embedded in glycol methacrylate (Polysciences, Warrington, PA, USA), sectioned, and stained with toluidine blue. To calculate pore diameters and porosity of the scaffolds, the stained images were processed and subjected to uneven lighting removal, image enhancement, binarization, and interference objectives removal, using pore topology analyzer software (MATLAB, Natick, MA, USA).15 (link) Using a universal testing machine (Z050; Zwick/Roell, Ulm, Germany) at a constant loading rate of 0.5 mm/min, the compressive strengths of the scaffold specimens (size of 10×5×3 mm³) were measured.16 The maximum point of the stress–strain curve was determined by the compressive strength. Measurements were carried out three times.

Wang X., Zhang G., Qi F., Cheng Y., Lu X., Wang L., Zhao J, & Zhao B. (2017). Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold. International Journal of Nanomedicine, 13, 117-127.

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