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Purogreen

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PuroGreen is a plasmid vector that confers puromycin resistance in transfected cells. It is commonly used for selection of successfully transfected cells.

Automatically generated - may contain errors

Lab products found in correlation

Polybrene, by Merck Group (2 mentions) Pwpxl, by Addgene (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

2 protocols using purogreen

1

Lentiviral-Mediated Ectopic Hsp27 Expression

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The Hsp27 ORF sequence (NM_001540.3) was PCR amplified using specific primers and cloned into the lentiviral expression vector pWPXL (Addgene). The specific shRNA sequences of Hsp27 were cloned into the vector PuroGreen (Addgene). Viral packaging was performed by cotransfection of pWPXL, pWPXL-Hsp27, shHsp27#1 or shHsp27#2 with packaging plasmid using Lipofectamine 2000 (Invitrogen) in HEK 293T cells. Viruses were harvested at 48h after transfection, and viral titers were determined. SK-Hep1, SMMC7721, MHCC97H, and HCCLM3 cells were infected with 1 × 106 recombinant lentivirus-transducing units in the presence of 6 μg/ml polybrene (Sigma).
Zhang Y., Tao X., Jin G., Jin H., Wang N., Hu F., Luo Q., Shu H., Zhao F., Yao M., Fang J., Cong W., Qin W, & Wang C. (2016). A Targetable Molecular Chaperone Hsp27 Confers Aggressiveness in Hepatocellular Carcinoma. Theranostics, 6(4), 558-570.
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2

Lentiviral Silencing of PDK4 in Liver Cancer Cells

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Stable SMMC7721 and HepG2 cells silenced for PDK4 expression were established using lentiviral constructs expressing shPDK4 and negative control. The specific shRNA sequences of PDK4 (Table S3) were cloned into the vector PuroGreen (Addgene) and vectors were packaged into 293T cells to produce viruses. Viruses were harvested 48h after transfection, and viral titers were determined. Target cells (1×105 cells) were infected with 1×106 recombinant lentivirus-transducing units in the presence of 6 μg/ml polybrene (Sigma, USA).
Yang C., Wang S., Ruan H., Li B., Cheng Z., He J., Zuo Q., Yu C., Wang H., Lv Y., Gu D., Jin G., Yao M., Qin W, & Jin H. (2019). Downregulation of PDK4 Increases Lipogenesis and Associates with Poor Prognosis in Hepatocellular Carcinoma. Journal of Cancer, 10(4), 918-926.
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