The Sf9 insect cell line, derived from the fall armyworm Spodoptera frugiperda, was purchased from Invitrogen (Carlsbad, CA, USA). Mouse monoclonal anti‐baculovirus glycoprotein gp64 IgG antibody (AcV5, sc‐65499) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). 1,2‐Dioleoyl‐sn‐glycero‐3‐phosphocholine (DOPC), 1,2‐dioleoyl‐sn‐glycero‐3‐phospho‐l ‐serine (DOPS), 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐(7‐nitro‐2‐1,3‐benzoxadiazol‐4‐yl) (NBD‐DOPE), 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐(lissamine rhodamine B sulfonyl) (Rho‐DOPE), and 1,2‐dioleoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐(Cyanine 5) (Cy5‐DOPE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA).
Sf9 insect cell line
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Sf9 insect cell line is a common cell line derived from the ovarian cells of the fall armyworm, Spodoptera frugiperda. This cell line is widely used in the field of molecular biology and biotechnology for the production of recombinant proteins and the study of various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl, by Avanti Polar Lipids (1 mentions)
Rho dope, by Avanti Polar Lipids (1 mentions)
Cy5 dope, by Avanti Polar Lipids (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Niacinamide, by Merck Group (1 mentions)
Kojic acid, by Merck Group (1 mentions)
Arbutin, by Merck Group (1 mentions)
Neutral red dye, by HiMedia (1 mentions)
Dimethyl sulphoxide, by Merck Group (1 mentions)
Cellfectin 2, by Thermo Fisher Scientific (1 mentions)
Tyrosine, by Merck Group (1 mentions)
Phenylmethanesulphonyl fluoride pmsf, by Merck Group (1 mentions)
Procollagen type 1 c peptide pip eia kit, by Takara Bio (1 mentions)
Calcein am, by Merck Group (1 mentions)
Bacpak baculovirus rapid titer kit, by Takara Bio (1 mentions)
Solvable, by PerkinElmer (1 mentions)
Micro bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
4 protocols using sf9 insect cell line
1
Insect Cell Culture and Lipid Labeling Protocol
2
Melanogenesis Inhibition Protocol
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4‐HR (Kumar Organics, Bangalore, India), 4‐butylresorcinol (4‐BR, TCI, Chennai, India), 4‐phenylethylresorcinol (4‐PER, Spechem, Chennai, India), hydrogen phosphate and potassium dihydrogen phosphate (S.D Fine‐Chemical Ltd, Mumbai, India), hydroquinone and potassium chloride (Merck, Darmstadt, Germany), sodium chloride (Fischer Scientific, Waltham, USA), ethanol absolute 99.9% (Commercial alcohols, Ontario, Canada) and tissue solubiliser ‐Solvable (Perkin Elmer, Boston, USA). Kojic acid, arbutin, niacinamide, disodium triton X 100, RIPA buffer, 3‐methyl benzothiazolinone‐2(3H)‐hydrazone (MBTH), 3,4‐dihydroxyphenylalanine (DOPA), tyrosine, phenylmethanesulphonyl fluoride (PMSF), glacial acetic acid, calcein‐AM, and dimethyl sulphoxide (DMSO) were procured from Sigma‐Aldrich, St. Louis, USA, and neutral red dye from HiMedia, Kennett Square, USA. Sf9 insect cell line, High Five (H5) insect cell line, and Cellfectin II were purchased from Invitrogen, Waltham, USA; BacPAK baculovirus rapid titer kit (Clontech, Mountain View, USA); procollagen Type I C‐peptide (PIP) EIA kit (Takara, San Jose, USA); micro‐BCA protein assay kit (Thermo Scientific, Waltham, USA).
3
Spodoptera frugiperda Cell Culture
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The Spodoptera frugiperda 9 (Sf9) insect cell line (Invitrogen USA) was utilized for baculovirus amplification and protein expression. A monolayer or suspension of Sf9 cells were seeded and cultured at a cell density ranging between 2.5 × 105 / ml − 1 × 106 using Ex-cell serum free medium containing 1% penicillin/streptomycin, 2% foetal calf serum (FCS), at 27°C with shaking for three-five days.
4
Establishment of Insect and Mammalian Cell Lines
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The Sf9 insect cell line derived from the fall armyworm Spodoptera frugiperda, commonly used in the baculovirus expression system, was purchased from Invitrogen (Waltham, CA, USA). HeLa cells were purchased from JCRB Bank (Japanese Collection of Research Biosources, Osaka, Japan); 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L -serine (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cyanine 5) (Cy5-DOPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-DOPE), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rho-DOPE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA).
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