5 mm sections were cut in a Leica Microsystems cryostat and transferred onto Superfrost-Plus slides (Thermo-Fisher Scientific), followed by staining with hematoxylin and eosin (H&E). For immunohistochemistry, the sections were deparaffinized and rehydrated, and antigen was retrieved using Dako target retrieval solution (Dako, Santa Clara, CA). To quench endogenous peroxidase activity incubation with 3% hydrogen peroxide for 30 min was performed. Specimens were incubated with cleaved caspase-3 antibody (Cell Signaling Technology), diluted in Dako antibody diluent overnight at 4 °C, followed by 3 washes with PBS/0.03% Tween-20 and incubation with Dako Labeled Polymer-HRP for 1 hr at room temperature. Following three washes with PBS containing 0.03% Tween-20, the sections were incubated with DAB chromogen and counterstained with hematoxylin.
Labeled polymer hrp
Manufactured by Agilent Technologies
Labeled Polymer-HRP is a reagent used in immunoassay and blotting applications. It consists of a polymer backbone conjugated with multiple horseradish peroxidase (HRP) labels. The HRP labels provide a strong signal amplification, enhancing the sensitivity of the detection method.
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Lab products found in correlation
2 protocols using labeled polymer hrp
1
Immunohistochemical Analysis of Apoptosis
2
Immunohistochemical Analysis of Apoptosis
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5 mm sections were cut in a Leica Microsystems cryostat and transferred onto Superfrost-Plus slides (Thermo-Fisher Scientific), followed by staining with hematoxylin and eosin (H&E). For immunohistochemistry, the sections were deparaffinized and rehydrated, and antigen was retrieved using Dako target retrieval solution (Dako, Santa Clara, CA). To quench endogenous peroxidase activity incubation with 3% hydrogen peroxide for 30 min was performed. Specimens were incubated with cleaved caspase-3 antibody (Cell Signaling Technology), diluted in Dako antibody diluent overnight at 4 °C, followed by 3 washes with PBS/0.03% Tween-20 and incubation with Dako Labeled Polymer-HRP for 1 hr at room temperature. Following three washes with PBS containing 0.03% Tween-20, the sections were incubated with DAB chromogen and counterstained with hematoxylin.
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