Gallic acid monohydrate

Chemical reagents used included
aluminum chloride (99% extrapure, anhydrous, granules), ethyl alcohol
(99% ACS spectroscopic grade), and quercetin hydrate (95%), which
were purchased from Thermo Fisher Scientific. Potassium acetate (certified
ACS crystalline) and sodium carbonate anhydrous (HPLC-grade powder)
were purchased from Fisher Scientific. Folin and Ciocalteu’s
phenol reagent and gallic acid monohydrate (ACS reagent grade) were
purchased from MP Biomedicals (Santa Ana, CA). Sodium acetate (Sigma
Ultra minimum 99.0%) was purchased from Sigma-Aldrich. Conc. HCl,
conc. NaOH, pure ethanol, and 95% ethanol were purchased from Fisher
Scientific. Distilled water was used for all procedures. Ultraviolet/visible
(UV/vis) determination of total concentrations of anthocyanins, flavonoids,
and polyphenols was performed on a Spectronic 10 Genesis spectrophotometer,
as described in the procedures below.

Chemical reagents used included
aluminum chloride (99% extra pure, anhydrous, granules), ethyl alcohol
(99% ACS spectroscopic grade), and quercetin hydrate (95%), which
were purchased from Thermo Fisher Scientific. Potassium acetate (certified
ACS Crystalline) and sodium carbonate anhydrous (HPLC grade powder)
were purchased from Fisher Scientific. Folin and Ciocalteu’s
Phenol reagent and gallic acid monohydrate (ACS reagent grade) were
purchased from MP Biomedicals (Santa Ana, CA). Sodium acetate (Sigma
Ultra minimum 99.0%) was purchased from Sigma-Aldrich. Amberlite resins
XAD1180 N, XAD7HP, XAD761, and FPX66 were purchased from Sigma-Aldrich.
Conc. HCl, Conc. NaOH, pure ethanol, and 95% ethanol were purchased
from Fisher Scientific. Distilled water was used for all procedures.
UV/Vis determination of total concentrations of anthocyanins, flavonoids,
and polyphenols was performed on a Spectronic 10 Genesis spectrophotometer,
as described in the procedures below.