Human osteosarcoma (HOS) and MCF7 cell lines were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany) and Normal Human Dermal Fibroblasts (NHDF) cell line from PromoCell, (Heidelberg, Germany); Eagle’s Minimal Essential Medium alpha (aMEM), Dulbecco’s Modified Eagle Medium (DMEM) without phenol red, 1% Penicillin–Streptomycin–Amphotericin B mixture (10K/10K/25 µg in 100 mL) and Trypsin–Versene (EDTA) mixture from Lonza, Verviers, Begium; fetal bovine serum (FBS) from Biochrom GmbH, Germany, CellTiter 96® Aqueous One Solution Cell Proliferation Assay from Promega (Madison, WI, USA); Tryple from Gibco, (Langley, VA, USA); LIVE/DEAD Viability/Cytotoxicity Kit and phosphate buffered saline (PBS) from Invitrogen, (Eugene, OR, USA), 35-mm Cell Imaging Dishes from Eppendorf (Hamburg, Germany).
Absorbance at 490 nm from MTS assay was measured with an iMark plate reader (Biorad, Hercules, CA, USA) and images for live/dead staining and wound scratch assays were recorded with a Leica DMI 3000B inverted microscope (Wetzlar, Germany).
Absorbance at 490 nm from MTS assay was measured with an iMark plate reader (Biorad, Hercules, CA, USA) and images for live/dead staining and wound scratch assays were recorded with a Leica DMI 3000B inverted microscope (Wetzlar, Germany).