Gartanin was obtained from Chromadex with 99% purity (Irvine, CA). MG-132 and MLN4924 were obtained from Cayman Chemical Inc. (Ann Arbor, MI). Antibodies against Ubc12, ubiquitin, and β-tubulin were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-Skp2 antibodies were purchased from Invitrogen (Grand Island, NY). Anti-Myc-tag, anti-LC3B-II, anti-FBXW2, anti-Cdh1, and anti-β-TrPC antibodies were from Cell Signaling Inc. (Boston, MA). Anti-Cullin-1, anti-FBW7 and anti-NEDD8 antibodies were from Abcam (Cambridge, MA). Anti-p27/Kip1 antibody was from BD Biosciences (Billerica, MA). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Sigma. Reverse Transcription System kit and was from Promega (Mandison, WI). A quantitative reverse transcription polymerase chain reaction (RT-PCR) kit was from Bio-Rad (Hercules, CA).
Quantitative reverse transcription polymerase chain reaction rt pcr kit
Manufactured by Bio-Rad
The Quantitative reverse transcription polymerase chain reaction (RT-PCR) kit is a laboratory instrument designed to perform real-time, quantitative analysis of gene expression. It is used to detect and quantify specific RNA molecules in a sample by converting the RNA to complementary DNA (cDNA) and then amplifying the target sequence using PCR technology.
Automatically generated - may contain errors
Lab products found in correlation
Anti myc tag, by Cell Signaling Technology (2 mentions)
Anti cullin 1, by Abcam (2 mentions)
Anti skp2 antibodies, by Thermo Fisher Scientific (2 mentions)
Anti nedd8, by Abcam (2 mentions)
Reverse transcription system kit, by Promega (2 mentions)
Mg132, by Cayman Chemical (2 mentions)
Mln4924, by Cayman Chemical (2 mentions)
Anti fbw7, by Abcam (1 mentions)
Anti lc3b 2, by Cell Signaling Technology (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Anti p27kip1 antibody, by BD (1 mentions)
Anti cdh1, by Cell Signaling Technology (1 mentions)
20s proteasome assay kit, by Cayman Chemical (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Anti cleaved parp, by Cell Signaling Technology (1 mentions)
Anti p27kip1, by BD (1 mentions)
Bortezomib, by Cayman Chemical (1 mentions)
2 protocols using quantitative reverse transcription polymerase chain reaction rt pcr kit
1
Gartanin-Mediated Protein Degradation
2
Proteasomal Regulation by FKB in Cancer
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FKB with 99% purity was isolated from kava extracts by LKT Laboratories, Inc. (St. Paul, MN). Bortezomib, MG-132 and MLN4924 were obtained from Cayman Chemical Inc. (Ann Arbor, MI). Antibodies against Ubc12, ubiquitin, and β-tubulin were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-Skp2 antibodies were purchased from Invitrogen (Grand Island, NY). Anti-Myc-tag and Anti-cleaved-PARP antibodies were from Cell Signaling (Boston, MA). Anti-Cullin-1 and anti-NEDD8 antibodies were from Abcam (Cambridge, MA). Anti-p27/Kip1 and p21/WAF1 antibodies were from BD Biosciences (Billerica, MA). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was purchased from Sigma. The Reverse Transcription System kit and was from Promega (Mandison, WI). A quantitative reverse transcription polymerase chain reaction (RT-PCR) kit was from Bio-Rad (Hercules, CA). Ubiquitylation Assay Kit and 20S Proteasome Assay Kit were from Cayman and Abcam, respectively.
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