Fitc anti cd34 clone 8g12

Manufactured by BD

FITC) anti-CD34 (clone 8G12; is a fluorochrome-conjugated monoclonal antibody that binds to the CD34 antigen. CD34 is a glycoprotein expressed on the surface of hematopoietic stem and progenitor cells. This product can be used for the identification and enumeration of CD34-positive cells in research applications.

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Lab products found in correlation

Diva software, by BD (2 mentions) Apc anti cd10 clone hi10a, by BD (2 mentions) Pe cyanine 7 pe cy7 anti cd38 clone hb 7, by BD (2 mentions) Apc h7 anti cd45ra clonehi100, by BD (2 mentions) Pe anti cd123 clone7g3, by BD (2 mentions) Facscanto 2, by BD (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

CD34-FITC (134 citations) Anti-CD34 (78 citations) Anti-CD34-FITC (44 citations) CD34 (clone 8G12), (6 citations) CD34-FITC (clone 8G12; (4 citations) CD34 FITC (8G12, (2 citations)

2 protocols using fitc anti cd34 clone 8g12

Flow Cytometric Analysis of AML EV-Treated CD34+ Cells

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After treatment with AML EVs, CD34⁺ cells were washed and incubated with APC anti-CD10 (clone HI10a, BD), fluorescein isothiocyanate (FITC) anti-CD34 (clone 8G12; BD), PE-Cyanine 7 (PE-Cy7) anti-CD38 (clone HB-7; BD), APC-H7 anti-CD45RA (cloneHI100; BD Pharmingen), peridinin chlorophyll (PerCP) anti-CD90 (IgG1k, Clone 5E10; BD Pharmingen), PE anti-CD123 (clone7G3; BD Pharmingen), APC anti-C-X-C chemokine receptor type 4 (CXCR4, clone 12G5; BD Pharmingen) monoclonal antibodies, at room temperature for 15 min in the dark. After incubation, cells were washed and resuspended in PBS. Then, 10,000 events were acquired on FACS CANTO II and analyzed by DIVA software (BD).

Trino S., Laurenzana I., Lamorte D., Calice G., De Stradis A., Santodirocco M., Sgambato A., Caivano A, & De Luca L. (2022). Acute Myeloid Leukemia Cells Functionally Compromise Hematopoietic Stem/Progenitor Cells Inhibiting Normal Hematopoiesis Through the Release of Extracellular Vesicles. Frontiers in Oncology, 12, 824562.

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Immunophenotypic Analysis of CD34+ Cells

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Fifty thousand EV-treated and untreated CD34⁺ cells were labeled with fluorescein isothiocyanate (FITC) anti-CD34 (clone 8G12; BD), PE-Cyanine 7 (PE-Cy7) anti-CD38 (clone HB-7; BD), APC-H7 anti-CD45RA (cloneHI100; BD Pharmingen), APC anti-CD10 (clone HI10a, BD), peridinin chlorophyll Cyanine 5.5 (PerCP-Cy5.5) anti-CD90 (Clone 5E10; BD Pharmingen), and PE anti- CD123 (clone7G3; BD Pharmingen) monoclonal conjugated antibodies, at RT for 15 min in the dark. After incubation, cells were washed and resuspended in PBS. Then, 10,000 events were acquired on the FACS CANTO II flow cytometer and analyzed by DIVA software (BD).

Laurenzana I., Trino S., Lamorte D., De Stradis A., Santodirocco M., Sgambato A., De Luca L, & Caivano A. (2021). Multiple Myeloma-Derived Extracellular Vesicles Impair Normal Hematopoiesis by Acting on Hematopoietic Stem and Progenitor Cells. Frontiers in Medicine, 8, 793040.

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