After heating all solutions to 90 °C under nitrogen flow, a 120 mM/100 mM aqueous solution of sodium hydroxide/potassium nitrate (9 mL) was mixed with a 1.3 M solution of iron sulphate heptahydrate in 0.01 M sulfuric acid using the NanoAssemblr Benchtop® system (Precision Nanosystems, Vancouver, Canada) in a 9:1 ratio at a rate of 10 mL/min.
Nanoassemblr benchtop system
Manufactured by Precision Nanosystems
Sourced in Canada
The NanoAssemblr Benchtop system is a laboratory instrument designed for the production of nanomaterials. It provides a controlled environment for the assembly of nanoparticles and other nanoscale structures. The system's core function is to facilitate the reliable and reproducible manufacture of nanomaterials for various research and development applications.
Automatically generated - may contain errors
3 protocols using nanoassemblr benchtop system
1
Synthesis of Iron Oxide Nanoparticles
2
Synthesis and Encapsulation of mRNA Vaccines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We synthesized mRNA vaccines encoding for the codon-optimized SARS-CoV-2 spike protein from USA-WA1/2020, OC43 spike protein, OVA from the SERPINB14 gene, HIV-1 SF162 envelope protein, or the LCMV GP. Constructs were purchased from Integrated DNA Technologies (IDT) or Genscript, and contained a T7 promoter site for in vitro transcription of mRNA. The sequences of the 5′- and −3′′-UTRs were identical to those used in a previous publication.44 All mRNAs were encapsulated into lipid nanoparticles using the NanoAssemblr Benchtop system (Precision NanoSystems) and confirmed to have similar encapsulation efficiency (~95%). mRNA was diluted in Formulation Buffer (Catalog # NWW0043, Precision NanoSystems) to 0.17 mg/mL and then run through a laminar flow cartridge with GenVoy ILM encapsulation lipids (Catalog # NWW0041, Precision NanoSystems) with N/P (Lipid mix/mRNA ratio of 4) at a flow ratio of 3:1 (RNA: GenVoy-ILM), with a total flow rate of 12 mL/min, to produce mRNA–lipid nanoparticles (mRNA-LNPs). mRNA-LNPs were evaluated for encapsulation efficiency and mRNA concentration using RiboGreen assay using the Quant-iT RiboGreen RNA Assay Kit (Catalog # R11490, Invitrogen, Thermo Fisher Scientific).
3
Synthesis of SARS-CoV-2 mRNA-LNP Vaccines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We synthesized mRNA vaccines encoding for the codon-optimized SARS-CoV-2 spike protein from USA-WA1/2020 or Omicron BA.1. Constructs were purchased from Integrated DNA Technologies (IDT) or Genscript, respectively, and contained a T7 promoter site for in vitro transcription of mRNA. The sequences of the 5′- and -3′′-UTRs were identical to those used in a previous publication.44 (link) All mRNAs were encapsulated into lipid nanoparticles using the NanoAssemblr Benchtop system (Precision NanoSystems) and confirmed to have similar encapsulation efficiency (∼95%). mRNA was diluted in Formulation Buffer (Catalog # NWW0043, Precision NanoSystems) to 0.17 mg/mL and then run through a laminar flow cartridge with GenVoy ILM encapsulation lipids (Catalog # NWW0041, Precision NanoSystems) with N/P (Lipid mix/mRNA ratio of 4) at a flow ratio of 3:1 (RNA: GenVoy-ILM), with a total flow rate of 12 mL/min, to produce mRNA–lipid nanoparticles (mRNA-LNPs). mRNA-LNPs were evaluated for encapsulation efficiency and mRNA concentration using RiboGreen assay using the Quant-iT RiboGreen RNA Assay Kit (Catalog # R11490, Invitrogen, Thermo Fisher Scientific). mRNA to express luciferase was purchased from TriLink Biotechnologies (CleanCap FLuc mRNA, L-7602, CleanCapFirefly Luciferase) and was encapsulated into lipid nanoparticles using the aforementioned protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!