The fluorescence susbstrates were purchased in DNA-synthesis, Moscow, Russia. Other oligonucleotides were purchased in Evrogen, Moscow, Russia. DNAse/RNAse free water was purchased from Qiagen, Hilden, Germany. Salts, including MgCl2, NaCl, KCl, HEPES, and DEPC were purchased from CarlROTH, Carlsruhe, Germany. Acrylamide was purchased from Applichem, Illinois, USA. Other materials for electrophoresis, including agarose, tris, boric acid, EDTA, bisAcrylamide, APS, TEMED, ethidium bromide, and SDS were purchased from Helicon, Moscow, Russia. Ladders and gel loading dyes were purchased from Evrogen, Moscow, Russia. Consumables for bacteria growth, including agar, tripton and yeast extract, were purchased in Dia-M, Moscow, Russia. Glass beads of technical grade was a gift from a technical department. Sodium acetate, phenol, and chlorophorm were purchased from Lenreactiv, Saint-Petersburg, Russia.
Acrylamide
Manufactured by AppliChem
Sourced in Germany
Acrylamide is a chemical compound used in various laboratory applications, including electrophoresis techniques, polymer synthesis, and as a monomer in the production of polyacrylamide gels. It serves as a core component in the preparation of these materials, which are widely utilized in analytical and biochemical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Dnase rnase free water, by Qiagen (1 mentions)
Sodium chloride (nacl), by Carl Roth (1 mentions)
Potassium chloride (kcl), by Carl Roth (1 mentions)
Agarose, by Helicon (1 mentions)
Ethidium bromide, by Helicon (1 mentions)
Yeast extract, by Dia-M (1 mentions)
Sodium dodecyl sulfate (sds), by Helicon (1 mentions)
Mgcl2, by Carl Roth (1 mentions)
Hepes, by Carl Roth (1 mentions)
Gdnhcl, by Nacalai Tesque (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Tween 20, by Carl Roth (1 mentions)
3 isobutyl 1 methylxanthine, by Merck Group (1 mentions)
Gamma globulin, by Merck Group (1 mentions)
Bicinchoninic acid (bca), by Merck Group (1 mentions)
N butanol, by Merck Group (1 mentions)
Bromophenol blue, by Merck Group (1 mentions)
Ethanol, by Carl Roth (1 mentions)
Betulinic acid, by Merck Group (1 mentions)
Sodium fluoride, by Merck Group (1 mentions)
4 protocols using acrylamide
1
Fluorescence Substrate Preparation Protocol
2
Protein Folding Monitoring Using ANS
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GdnHCl (Nacalai Tesque, Japan), acrylamide (AppliChem, Germany), and ANS (ammonium salt of 8-anilinonaphtalene-1-sulfonic acid; Fluka, Switzerland) were used without further purification. The protein concentration was 0.1–0.2 mg/ml. The experiments were performed in 20 mM Na-phosphate-buffered solution at pH 7.8.
3
Radioimmunoassay Protocol for cGMP Quantification
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125I-cGMP with a specific activity of 81.4 TBq/mmol was obtained from Biotrend (Cologne, Germany). 2-Mercaptoethanol, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), acetic acid, ethanol, glucose, isopropyl alcohol, milk powder, methanol, N,N,N’,N’-tetramethylethylene-diamine (TEMED), sodium acetate, sodium dodecyl sulfate (SDS), Tris-buffer, and Tween® 20 were obtained from Roth (Karlsruhe, Germany). 3-Isobutyl-1-methylxanthine (IBMX), 3-morpholino-sydnonimine (SIN-1), ammonium persulfate (APS), betulinic acid (BA), bicinchoninic acid, bromophenol blue, copper sulfate, gamma-globulin, n-butanol, sepiapterin, sodium fluoride, sodium pyrophosphate, and Triton-X 100 were obtained from Sigma (Taufkirchen, Germany). Acrylamide, 30% solution (37.5:1), and bovine serum albumin (BSA), albumin fraction V were obtained from Applichem (Darmstadt, Germany). ICI 182,780 was obtained from Tocris Bioscience (Minneapolis, MN, USA). LY294002, and wortmannin were obtained from Calbiochem (Darmstadt, Germany).
4
Protein Modification and Characterization
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Acrylamide (AppliChem GmbH, Darmstadt, Germany); bis-Acrylamide (Amresco, Solon, OH, USA); HSA (fraction V; Renal, Budapest, Hungary); Nile Red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one (C20H18N2O2)) (Merck, Darmstadt, Germany); 1 M MgCl2 (M1028-1ML) (Sigma, St. Louis, MO, USA); NaCl, (NH4)2S2O8 (Acros Organics, Waltham, MA, USA); N,N,N′,N′-tetramethyl ethylenediamine (TEMED) (Bio-Rad Laboratories, Berkeley, CA, USA); Tris (Fisher Scientific, Pittsburgh, PA, USA); AcOH (ice-cold) and Stains-All (Acros Organics, Waltham, MA, USA); xylene cyanol (Sigma, St. Louis, MO, USA); Ficoll 400 (Pharmacia, Stockholm, Sweden); DMEM; DMEM F12; Fetal Bovine Serum (FBS); GlutaMAX Supplement; Antibiotic-Antimycotic; and TrypLE (Gibco, Waltham, MA, USA) were used in this work. The isolation and purification of the HSA monomeric fraction were performed according to [68 (link)]. HSA modified by sulfo-Cy5 dye (HSA-Cy5) was obtained according to [69 (link)]. For the solutions, Milli-Q (18.2 M × Om/cm) water (purified by Simplicity 185 water system (Millipore, St. Louis, MO, USA)) was used.
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