Hla abc fitc

The cells were stained with fluorescent dye–conjugated antibodies for 30 min on ice and washed twice with PBS. After resuspending in PBS containing 2% FBS, the cells were analyzed by fluorescence-activated cell sorting (FACS Calibur; BD Biosciences, Franklin, NJ, USA). The following antibodies were used in the analysis: CD34-FITC, CD73-FITC, CD90-FITC, CD90-PE, human leukocyte antigen (HLA)-ABC-FITC, HLA-DR-FITC (eBioscience, San Diego, CA, USA); CD44-FITC, CD105-APC (BD Biosciences); CD45-FITC (BioLegend, San Diego, CA, USA). In the case of fresh tissues, CD73-FITC, CD90-PE, and CD105-APC were used for triple staining. Antibodies against PD-L1-PE and PD-L2-APC were purchased from eBioscience.