pfLuc-T21 contains four tandem repeats of the reverse complement of the hsa-miR-21 sequence separated by 8-nucleotide spacers (T21), while pfLuc-Tcon contains four tandem repeats of the hsa-miR-21 reverse sequence in the same configuration (Tcon). Both plasmids were constructed by insertion of annealed complementary oligonucleotides into the 3’UTR of the firefly luciferase (fLuc) gene in pMIR-REPORT” (miRNA Expression Reporter Vector System; Ambion, Austin, TX). Oligonucleotides were T21F, T21R, TconF, and TconR (Table 1 ). Annealed oligonucleotides were digested with SpeI and SacI, and ligated to SpeI-SacI-digested pMIR-REPORT™.
Mirna expression reporter vector system
Manufactured by Thermo Fisher Scientific
The MiRNA Expression Reporter Vector System is a tool for studying microRNA (miRNA) expression. It provides a vector system for the cloning and expression of miRNA precursors and reporter constructs for miRNA detection and quantification.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mirna expression reporter vector system
1
Plasmid Construction for miR-21 Study
2
Plasmid Construction for miR-21 Study
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pfLuc-T21 contains four tandem repeats of the reverse complement of the hsa-miR-21 sequence separated by 8-nucleotide spacers (T21), while pfLuc-Tcon contains four tandem repeats of the hsa-miR-21 reverse sequence in the same configuration (Tcon). Both plasmids were constructed by insertion of annealed complementary oligonucleotides into the 3’UTR of the firefly luciferase (fLuc) gene in pMIR-REPORT” (miRNA Expression Reporter Vector System; Ambion, Austin, TX). Oligonucleotides were T21F, T21R, TconF, and TconR (Table 1 ). Annealed oligonucleotides were digested with SpeI and SacI, and ligated to SpeI-SacI-digested pMIR-REPORT™.
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