Photographs for Fig. 3b and Fig. 4a–d were taken using a Canon EOS 60D camera with an EF-S60mm f/2.8 Macro USM lens and a Hoya 52 mm Circular Polarizing Pro 1 digital multi-coated glass filter; Cognisys Stackshot 3X Macro Rail Package and Helicon Focus 6.7.1 Pro were used to z-stack images. Photographs for Fig. 4e–g and Supplementary Figs. 4 –8 were taken using a Nikon D300 camera. Composite images were stitched using Adobe Photoshop 2021.
D300 camera
Manufactured by Nikon
Sourced in Japan, Germany
The Nikon D300 is a digital single-lens reflex (DSLR) camera. It features a 12.3-megapixel DX-format CMOS sensor, a 3-inch LCD display, and a 51-point autofocus system. The camera supports recording of high-definition video and provides a range of manual controls for photographers.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 510 meta, by Zeiss (2 mentions)
Axiophot light microscope, by Zeiss (2 mentions)
Volocity, by PerkinElmer (1 mentions)
Dp74 camera, by Olympus (1 mentions)
Discovery ultra system, by Roche (1 mentions)
Vip 6 tissue tektissue processor, by Sakura Finetek (1 mentions)
Discovery dab map detection kit, by Roche (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Illustrator cc 2019, by Adobe (1 mentions)
Coolsnap camera, by Teledyne (1 mentions)
Leica m125c, by Leica Microsystems (1 mentions)
Szx12, by Olympus (1 mentions)
Axiophot microscope, by Zeiss (1 mentions)
Optiphot, by Nikon (1 mentions)
Sucrose, by Merck Group (1 mentions)
14 protocols using d300 camera
1
Optimized Macro Photography Workflow
2
Confocal and Widefield Imaging Analysis
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Confocal images were captured on a NikonA1R confocal microscope at 20X or 60X magnification. Widefield image sections were captured at 20X on a Zeiss Axioplan II and images deconvolved using Volocity (PerkinElmer). For quantitative analysis, imaged were taken using the same acquisition parameters. Brightfield colour images of heads, wings and notum were acquired using an Olympus SX9 stereomicroscope (4X) attached to a Nikon D300 camera. Image J was used to create a black overlay mask by thresholding the GFP channel images. The subsequent black mask corresponding to GFP negative regions was then superimposed over the Ci155 or βGal images. ImageJ was also used for measuring adult heads, band densitometry and pixel intensity. Microsoft Excel and GraphPad Prism were used for graphs and ANOVA and t-test statistical analysis.
3
Longitudinal Ocular Alignment Measurement
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Ocular alignment was measured longitudinally using Hirschberg photographic methods during the 16 weeks of prism-rearing (first photos at ∼11 day of age after transfer to the University of Houston facility), and for 18 weeks after the prism-rearing period.35 (link)–37 (link) Digital photographs of the infant animals were acquired twice a week in a dimly illuminated room using a D300 camera (Nikon, Tokyo, Japan) with an attached ring light placed at a distance of 60 cm from the animal in primary gaze. To facilitate photography, the infant animals were wrapped in a blanket and placed in a primate chair (Crist Instrument Co, Inc., Hagerstown, MD), while one of the investigators lightly held the animal's head to prevent excessive movement. Prism helmets were removed for the duration of the photographic procedure, and multiple photographs were taken during each session for each monkey. Considering a previous report suggesting that even 60 minutes of normal binocular vision each day might influence the final state of strabismus,38 (link),39 (link) we were careful to minimize the overall photography time. The time spent under binocular vision, owing to the experimental procedure, was also recorded.
4
Klebsiella pneumoniae Histopathological Analysis
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Necropsies were performed by veterinary pathologists on predetermined endpoint days. During necropsy, lungs were assessed for gross pathology and images were captured with a Nikon D300 camera. Tissue samples were also collected for histopathological analysis. Samples were fixed in 10% neutral buffered formalin (Cancer Diagnostics, Durham, NC) for a minimum of 72 h and processed using a VIP-6 Tissue Tektissue processor (Sakura Finetek, USA). Tissue samples were embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC) and sectioned serially at 5 μm. Air-dried slides were stained with hematoxylin and eosin. K. pneumoniae was visualized within tissue sections using rabbit anti-K. pneumoniae CPS2 IgG (1:500 dilution) (14 (link)) and a Discovery ULTRA System (Roche Diagnostics) and Discovery DAB Map detection kit (Roche Diagnostics) according to the manufacturer’s protocol. Images were obtained using an Olympus BX51 microscope and an Olympus DP74 camera (Olympus Corporation). Image brightness and contrast were evenly adjusted using Adobe Photoshop, and figures were created using Adobe Illustrator CC 2019 (Adobe, San Jose, CA).
5
Microscopic Imaging of Plant Seedlings
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Bright-field and fluorescence images of seedlings were collected with a Leica MZFLIII microscope and CoolSNAP camera (Roper Scientific) or Nikon D300 camera using Qcapture software. Confocal images were acquired on Leica SP5 or Zeiss LSM510META, and long-term imaging was performed under perfluorodecalin (F2 Chemicals) in Carolina Gel (Blades Biological) chambers. Immunoelectron microscopy was performed as previously described (Dettmer et al., 2006 (link)) on ultrathin thawed cryosections of formaldehyde-fixed lateral roots.
6
Standardized Surgical Documentation
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We photo-documented relevant surgical steps under standardized conditions using a Nikon D300 camera equipped with a Vario lens. We used the integrated FullHD camera (1920 px × 1080 px) of two marLED X operating lights (KLS Martin, Tuttlingen, Germany) for video documentation.
7
Morphometric Analysis of Stickleback Fish
8
Leaf Imaging with Stereomicroscopes
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We used one of two different stereomicroscopes, a Leica M125 C (Leica Microsystems, Wetzlar, Germany) with a Leica MC170 HD camera or an Olympus SZX12 (Olympus, Tokyo, Japan) with a Nikon D300 camera (Nikon, Tokyo, Japan), to image leaves under transmitted light. We assumed image quality from each microscope was comparable. The leaves were kept hydrated throughout the imaging process using 70% ethanol. We imaged each leaf three times: once at the base, middle, and apex of the lamina using 10× or 12.5× magnification.
9
Inducing fz or ft overexpression in Drosophila
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To induce clones overexpressing fz or ft, pupae of the appropriate genotypes were heat shocked, at 96–120 h after egg deposition, at 37°C for 30 min in a water bath. Between 2 and 3 days after eclosion, adult flies were selected and kept in tubes containing 70% ethanol. Cuticles were dissected and mounted in Hoyers medium. Images were taken on a Zeiss Axiophot microscope (Carl Zeiss Ltd, Cambourne, UK) equipped with Nomarski optics using a 40x/0.90 Plan-Neofluar lens, a Nikon D-300 camera (Nikon Uk Ltd, Surbiton, UK) connected to an iMac computer and Nikon Camera Control Pro 2. Stacks of images taken at different focal planes were combined into a single image with Helicon Focus (HeliconSoft, Kharkiv, Ukraine).
10
Photographic Documentation of Novel Pastry Designs
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Following the current pastry trends [42 (link)], five cakes were designed by “Casa La Curra” (Torrent, Valencia, Spain) to be used in this study (Figure 1 ). “Leonor cake” (LC) was made of an almond base and lemon mousse and decorated with candied orange pieces (Figure 1 a). “Saffron cake” (SC) consisted of a base of butter biscuits filled with saffron cream and a chocolate mousse (Figure 1 b). “Walnut cake” (WC) was made of a sablée breton with salted caramel cream and nuts (Figure 1 c). A “Coulant cake” (CC) with chocolate and dried raspberries (Figure 1 d) was created. Finally, the “Chocomuffin” (CM) is a traditional muffin filled with chocolate (Figure 1 e).
Pictures were taken with a Nikon D300 camera with Nikkor 24–70 mm f/2.8S objective (Nikon Corporation, Tokyo, Japan). A white uniform background and three led panel light Neewer (Shenzhen Neewer Technology Co., Shenzhen, China) were used to ensure constant lighting conditions.
Pictures were taken with a Nikon D300 camera with Nikkor 24–70 mm f/2.8S objective (Nikon Corporation, Tokyo, Japan). A white uniform background and three led panel light Neewer (Shenzhen Neewer Technology Co., Shenzhen, China) were used to ensure constant lighting conditions.
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