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Anti phospho s6 ribosomal protein ser240 244 d68f8

Manufactured by Cell Signaling Technology
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The Anti-phospho S6 ribosomal protein (Ser240/244) (D68F8) is a rabbit monoclonal antibody that specifically recognizes the phosphorylated form of the S6 ribosomal protein at serine residues 240 and 244. This antibody is useful for detecting the phosphorylation status of the S6 ribosomal protein, which is a downstream target of the mTOR signaling pathway.

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Lab products found in correlation

Anti pser473 akt, by Cell Signaling Technology (1 mentions) Ecl system, by Cytiva (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti p27 610241, by BD (1 mentions) Anti vinculin n 19, by Santa Cruz Biotechnology (1 mentions) Anti caspase 7, by Cell Signaling Technology (1 mentions) Anti bcl xl 54h6, by Cell Signaling Technology (1 mentions) Anti mcl 1 s19, by Santa Cruz Biotechnology (1 mentions) Dinaciclib, by Selleck Chemicals (1 mentions) A 1210477, by Chemietek (1 mentions) Ct a199, by Chemietek (1 mentions) Anti bim c34c5, by Cell Signaling Technology (1 mentions) Anti cleaved parp asp214 d64e10, by Cell Signaling Technology (1 mentions) Anti phospho her2 erbb2 tyr1248, by Cell Signaling Technology (1 mentions) Anti phospho akt ser473 d9e, by Cell Signaling Technology (1 mentions) Anti phospho akt thr308 244f9, by Cell Signaling Technology (1 mentions) Anti phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti bak, by Cell Signaling Technology (1 mentions) S2768, by Selleck Chemicals (1 mentions) Anti gapdh 6c5, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Phospho-S6 (110 citations) S6 ribosomal protein (94 citations) Anti-S6 (87 citations) Anti-phospho-S6 (60 citations) Phospho-S6 ribosomal protein (52 citations) Anti-S6 ribosomal protein (26 citations) Phospho-S6 Ribosomal Protein (Ser240/244) (20 citations) Phospho-S6 (Ser240/244) (16 citations) Anti-phospho-S6 ribosomal protein (16 citations) Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) (7 citations)

2 protocols using anti phospho s6 ribosomal protein ser240 244 d68f8

1

Protein Extraction and Western Blotting

Cited 1 time
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Protein extraction and western blotting were performed as described elsewhere.58 (link) Antibodies directed against HMGA1 proteins were already described.59 (link) Commercial antibodies were: anti-pSer473-AKT (#9271), anti-AKT (#9272), anti-Caspase-7 (#9492), anti-phospho S6 ribosomal protein (Ser240/244) (D68F8, #5364), anti-S6 ribosomal protein (54D2, #2317S), anti-LC3A/B (#4108), anti-PARP (46D11, #9532), anti-ATG5 (D5F5U, #12994) and anti-ULK1 (R600, #4773) antibodies from Cell Signaling Technology (MA, USA); anti-β-Actin (I-19, sc-1616), anti-β-Tubulin (D-10, sc-5274), anti-p21 (C-19, sc-397), anti-Cyclin D1 (A12, sc-8396), anti-Cyclin E (E20, sc-481), and anti-Vinculin (N-19, sc-7649) were from Santa Cruz Biotechnology (Dallas, TX, USA); anti-p27 (610241) and anti-p62/SQSTM1 (610833) were from BD Biosciences (Franklin Lakes, NJ USA); anti-phospho ATG13 (Ser318) (PAB19948) was from Abnova (Taipei City,Taiwan); anti-ATG13 (SAB4200100) was from Sigma-Aldrich. ECL System was purchased from Amersham Pharmacia (Buckinghamshire, UK).
Conte A., Paladino S., Bianco G., Fasano D., Gerlini R., Tornincasa M., Renna M., Fusco A., Tramontano D, & Pierantoni G.M. (2017). High mobility group A1 protein modulates autophagy in cancer cells. Cell Death and Differentiation, 24(11), 1948-1962.
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2

Targeted Cancer Therapy Compounds and Antibodies

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The following drugs were purchased: Dinaciclib (SCH727965) for in vitro and in vivo studies (S2768; Selleckchem), lapatinib ditosylate (Tykerb) for in vitro and in vivo studies (M1802; Abmole), neratinib for in vivo studies (M1913; Abmole), A-1210477 (CT-A121; Chemietek), A-1331852 (22963; Cayman Chemicals), tucatinib (HY-16069; Medchem), and ABT-199 (venetoclax) (CT-A199; Chemietek). The antibodies used in this study were as follows: anti-Bak (AB-1 clone for IP) (AM03; EMD Millipore), anti-Bak (3814S; Cell Signaling), anti-Bim (C34C5) (2933S; Cell Signaling), anti–BCL-xL (54H6) (2764S; Cell Signaling), anti–Bcl-2 (D55G8) (Human Specific) (4223S; Cell Signaling), anti-cleaved PARP (Asp214) (D64E10) (5625S; Cell Signaling), anti-GAPDH (6C5) (sc-32233; Santa Cruz), anti–MCL-1 (S-19) (sc-819; Santa Cruz), anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) (4370S; Cell Signaling), anti-phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) (5364S; Cell Signaling), anti-phospho-Akt (Thr308) (244F9) (4056S; Cell Signaling), anti-phospho-Akt (Ser473) (D9E) (4060S; Cell Signaling), anti-HER2/ErbB2 (29D8) (2165S; Cell Signaling), anti-phospho-HER2/ErbB2 (Tyr1248) (2247S; Cell Signaling), anti-phospho-Rpb1 CTD (Ser 2/5) (4375S; Cell Signaling), Normal Rabbit IgG for IP (sc-2027; Santa Cruz), and Normal Mouse IgG for IP (sc-2025; Santa Cruz).
Floros K.V., Jacob S., Kurupi R., Fairchild C.K., Hu B., Puchalapalli M., E. Koblinski J., Dozmorov M.G., Boikos S.A., Scaltriti M, & Faber A.C. (2021). Targeting transcription of MCL-1 sensitizes HER2-amplified breast cancers to HER2 inhibitors. Cell Death & Disease, 12(2), 179.
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