Masstrak kit

Manufactured by Waters Corporation

The MassTrak kit is a laboratory equipment product offered by Waters Corporation. It is designed for the analysis and quantification of compounds in various samples. The kit provides the necessary components and protocols for performing mass spectrometry-based analytical procedures.

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Lab products found in correlation

Tsq quantum ultra mass spectrometer, by Waters Corporation (2 mentions) Acquity uplc system, by Waters Corporation (2 mentions) Tsq quantum ultra, by Waters Corporation (1 mentions) Acquity, by Waters Corporation (1 mentions)

5 protocols using masstrak kit

Quantifying Sulfur-Containing Amino Acids

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Serine, homoserine, lanthionine and cystathionine – all amino acids produced concurrently with and proxies for
hydrogen sulfide – were measured by LC-MS, similar to our amino acid plus metabolites panel as referenced
[49 (link)]. Tissue homogenate samples were spiked with internal standards
(10 μl of serine, homserine, lanthionine, and cystathionine each at a concentration of 2 μg/ml), then
deproteinized with cold me-thanol followed by centrifugation at 10,000g for 15 min. The supernatant was
immediately derivatized with 6-aminoquinolyl-N-hydro-xysuccinimidyl carbamate according to Waters’ MassTrak kit. An
11-point calibration curve underwent similar derivatization procedure after the addition of internal standards. Both
derivatized standards and samples were analyzed on a triple quadrupole mass spectrometer (Thermo TSQ Quantum Ultra) coupled
with an Ultra Pressure Liquid Chromatography (Waters Acquity) system. Data acquisition was done using select ion monitor
(SRM). The following transitions (m/z) were monitored: 276.25 > 171.04 for serine, 290.2
> 171.04 for homoserine, 275.2 > 171.04 for lanthionine and 282.3 > 171.04 for cystathionine. Concentrations of the
analytes of each unknown were calculated against each respective calibration curve.

Hale V.L., Jeraldo P., Mundy M., Yao J., Keeney G., Scott N., Cheek E.H., Davidson J., Green M., Martinez C., Lehman J., Pettry C., Reed E., Lyke K., White B.A., Diener C., Resendis-Antonio O., Gransee J., Dutta T., Petterson X.M., Boardman L., Larson D., Nelson H, & Chia N. (2018). Synthesis of multi-omic data and community metabolic models reveals insights into the role of hydrogen sulfide in colon cancer. Methods (San Diego, Calif.), 149, 59-68.

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Quantitative Urine Metabolomics Analysis

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Urine samples were spiked with internal standards then deproteinized with cold methanol followed by centrifugation at 10,000 × g for 5 min. The supernatant was immediately derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate according to Waters' MassTrak kit. A 10-point calibration standard curve underwent a similar derivatization procedure after the addition of internal standards. Both derivatized standards and samples were analyzed on a triple quadrupole mass spectrometer coupled with an Ultra Pressure Liquid Chromatography system. Data acquisition was done using select ion monitor (SIM). Concentrations of 47 analytes, including 5 neurotransmitters and 42 other amino metabolites, in each sample were calculated against each perspective calibration curve.

Cheng Y., Song H., Pan X., Xue H., Wan Y., Wang T., Tian Z., Hou E., Lanza I.R., Liu P., Liu Y., Laud P.W., Usa K., He Y, & Liang M. (2018). Urinary Metabolites Associated with Blood Pressure on a Low- or High-Sodium Diet. Theranostics, 8(6), 1468-1480.

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LCMS-based Quantification of Neurotransmitters

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Neurotransmitters and some amino acids such as Tryptophan and Taurine were measured by LCMS as previously described (37 (link)). Briefly, plasma samples were spiked with an internal standard mix consisting of isotopically labeled amino acids. They were then deproteinized with cold methanol followed by centrifugation at 12,000 g for 10 minutes. The supernatant was transferred to a different vial, dried down and derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate according to Waters’ MassTrak kit. A 11-point calibration standard curve was spiked with the same internal standard mix and underwent the same derivatization procedure. Both derivatized standards and samples were analyzed on a Thermo TSQ Quantum Ultra mass spectrometer (West Palm Beach, FL) coupled with a Waters Acquity UPLC system (Milford, MA). Data acquisition was done using selective ion monitor (SRM). Concentrations of the analytes of each unknown were calculated against their respective calibration curves (37 (link)).

Pak V.M., Dai F., Keenan B.T., Gooneratne N, & Pack A.I. (2017). Lower Plasma Choline Levels are Associated with Sleepiness Symptoms. Sleep medicine, 44, 89-96.

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Quantitative Amino Acid Profiling by LCMS

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Amino acids and their metabolites were measured by LCMS as
previously described^{42 (link),43}. Briefly, 50μl of
either cell media or 1xPBS was added to the cell pellets followed by
addition of internal standard solution. The sample mixture was sonicated and
deproteinized with cold methanol followed by centrifugation at 18,000g for
15 minutes. The supernatant was dried down and then derivatized with
6-aminoquinolyl-N-hydroxysuccinimidyl carbamate according to Waters’
MassTrak kit. A 11-point calibration standard curve underwent similar
derivatization procedure after the addition of internal standards. Both
derivatized standards and samples were analyzed on on the Thermo TSQ Quantum
Ultra mass spectrometer (West Palm Beach, FL) coupled with a Waters Acquity
UPLC system (Milford, MA). Data acquisition was done using select ion
monitor (SRM). Concentrations of 42 analytes of each unknown were calculated
against each perspective calibration curve.

Arneson-Wissink P.C, & Doles J.D. (2021). Disrupted NOS2 metabolism drives myoblast response to wasting-associated cytokines. Experimental cell research, 407(1), 112779.

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Targeted Amino Acid Profiling by LC-MS

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Amino acids and their metabolites were measured by LCMS as previously described (Lanza et al., 2010 (link)). Briefly, 20 μl of plasma was spiked with an internal standard solution consisting of isotopically labeled amino acids. The supernatant was immediately derivatized with 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate according to Waters' MassTrak kit. A 10‐point calibration standard curve underwent similar derivatization procedure after the addition of internal standards. Both derivatized standards and samples were analyzed on a Thermo Quantum Ultra triple quadrupole mass spectrometer coupled with a Waters Acquity liquid chromatography system. Data acquisition was done using select ion monitor (SRM) via positive electrospray condition. Concentrations of 42 analytes of each unknown were calculated against its perspective calibration curve.

Gries K.J., Hart C.R., Kunz H.E., Ryan Z., Zhang X., Parvizi M., Liu Y., Dasari S, & Lanza I.R. (2022). Acute responsiveness to single leg cycling in adults with obesity. Physiological Reports, 10(24), e15539.

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