Sections were dewaxed, rehydrated and antigen retrieval was performed with 10 mM sodium citrate (pH 6.1). Next, the sections were blocked with 5% bovine serum albumin for 20 mins at 37°C. The sections were incubated with anti-FIZZ1 (1:300), anti-type I collagen (1:300), anti-E-cadherin (l:300) or anti-fibronectin-1 (l:300) antibodies (all Santa Cruz Biotechnology, Inc.) overnight at 4°C. The sections were subsequently incubated with polyclonal goat anti-rabbit immunoglobulins/horseradish peroxidase (1:200) for 30 min at 37°C. The nuclei were counterstained with hematoxylin.
Anti type 1 collagen
Manufactured by Santa Cruz Biotechnology
Anti-type I collagen is a laboratory product used for the detection and quantification of type I collagen in various biological samples. It is a specific antibody that binds to the type I collagen protein, allowing for its identification and measurement.
Automatically generated - may contain errors
2 protocols using anti type 1 collagen
1
Immunohistochemistry Staining Protocol
2
Lung Protein Immunoblotting Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In total, 30 μg protein extracted from the lung was separated by 10% SDS-PAGE, transferred onto a polyvinylidene fluoride membrane and subjected to immunoblotting with anti-α-SMA, anti-type I collagen or anti-β-actin antibodies (Santa Cruz Biotechnology, Inc.).
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