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Sc 8628

Manufactured by Abcam
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Sc-8628 is a primary antibody produced in mouse that recognizes a specific target. It can be used in various immunoassays to detect the presence and quantity of the target protein.

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Lab products found in correlation

Ab209665, by Abcam (1 mentions) Af1700, by R&D Systems (1 mentions) Af1924, by R&D Systems (1 mentions) Sc 5279, by Santa Cruz Biotechnology (1 mentions) Af1979, by R&D Systems (1 mentions) Mab2018, by Abcam (1 mentions) Ab150079, by Abcam (1 mentions) Ab45932, by Abcam (1 mentions) Ab150108, by Abcam (1 mentions) Ab97959, by Santa Cruz Biotechnology (1 mentions) Ab16287, by Abcam (1 mentions) Ab150113, by Thermo Fisher Scientific (1 mentions) Asc 005, by Abcam (1 mentions) A110694, by Merck Group (1 mentions) Sc 33759, by Abcam (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Nis elements ar software, by Nikon (1 mentions) Ab29112, by Abcam (1 mentions) Ab24273, by Abcam (1 mentions) C7207, by Merck Group (1 mentions)

3 protocols using sc 8628

1

Pluripotency Marker Expression Profiling

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SOX2 (1:100; R&D systems, MAB2018), TFAP2C (1:500, Abcam, ab218107 or 1:500, Santa Cruz, Sc12762 or 1:100, R&D systems, AF5059), OCT4 (1:200; Santa Cruz, sc5279 or 1:300, Santa Cruz, sc8628), TBXT (1:500; Abcam, ab209665), GATA2 (1:500; Abcam, ab173817), NANOG (1:400, Cell Signalling, 4893), KRT7 (1:100, Thermo Fisher, MA5-11986), GATA6 (1:100, R&D systems, AF1700), SOX17 (1:100, R&D systems, AF1924), OTX2 (1:300, R&D systems, AF1979).
Bergmann S., Penfold C.A., Slatery E., Siriwardena D., Drummer C., Clark S., Strawbridge S.E., Kishimoto K., Vickers A., Tewary M., Kohler T.N., Hollfelder F., Reik W., Sasaki E., Behr R, & Boroviak T.E. (2022). Spatial profiling of early primate gastrulation in utero. Nature, 609(7925), 136-143.
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2

Immunofluorescence Staining of Stem Cell and Cardiomyocyte Markers

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Cells were fixed with 4% paraformaldehyde (PFA) for 15 min, permeabilized with 0.1% Triton X-100 (Sangon Biotech, A110694) for 5 min, and blocked with 3% bovine serum albumin (Sigma-Aldrich, A1933) for 1 h. Cells were subsequently stained with appropriate primary antibodies and AlexaFluor conjugated secondary antibodies. Primary antibodies include NANOG (Santa Cruz Biotechnology, sc-33759, 1:200), SOX2 (Abcam, ab97959, 1 μg/ml), OCT4 (Santa Cruz Biotechnology, sc-8628, 1:500), SSEA-4 (Abcam, ab16287, 1:500) TNNT2 (Abcam, ab45932, 1:400), α-actinin (Abcam, ab137346, 1:500), Nav1.5 (Alomone labs, ASC-005, 1:200) and β-catenin (Abcam 237983, 1ug/ml). Secondary antibodies include AlexaFluor® 647 (Abcam, ab150079, 1:500), AlexaFluor® 594 (Abcam, ab150108, 1:500) and AlexaFluor® 488 (Abcam, ab150113, 1:500; Invitrogen, A11008, 1:500). Nuclei were stained with DAPI (Roche Diagnostics, 1023276001, 1 μg/ml). Pictures were taken with 60 × objective on confocal microscope (Nikon, A1) using NIS-Elements AR software (Nikon).
Cai D., Wang X., Sun Y., Fan H., Zhou J., Yang Z., Qiu H., Wang J., Su J., Gong T., Jiang C, & Liang P. (2023). Patient-specific iPSC-derived cardiomyocytes reveal aberrant activation of Wnt/β-catenin signaling in SCN5A-related Brugada syndrome. Stem Cell Research & Therapy, 14, 241.
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3

Pluripotency Marker Immunostaining Protocol

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Cells were fixed with paraformaldehyde, permeabilized, and stained with primary antibodies against OCT4 (Santa Cruz Biotechnology; sc-8628), NANOG (Abcam; ab808992), UTF1 (Abcam; ab24273), SALL4 (Abcam; ab29112), LIN28 (Abcam; ab46020), and CTNNB1 (Sigma-Aldrich; C7207) followed by staining with appropriate secondary antibodies conjugated to either Alexa Fluor 555 or 647 (Invitrogen). Images were acquired with a Neo 5.5 cSMOS camera (Andor) and processed and analyzed using NIS-Elements and Adobe Photoshop.
Vidal S.E., Amlani B., Chen T., Tsirigos A, & Stadtfeld M. (2014). Combinatorial Modulation of Signaling Pathways Reveals Cell-Type-Specific Requirements for Highly Efficient and Synchronous iPSC Reprogramming. Stem Cell Reports, 3(4), 574-584.
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