Testosterone cypionate, nandrolone decanoate, and boldenone undecylenate were purchased from Steraloids Inc (Newport, RI) and prepared in sesame oil. Eticlopride hydrochloride (ETIC), serotonin hydrochloride (5HT) and arginine vasopressin (AVP) were purchased from Sigma Aldrich (St. Louis, MO). The ETIC and AVP were dissolved in 0.9% (wt/vol) normal saline, and the 5HT was dissolved in ddH2O.
Testosterone cypionate
Manufactured by Steraloids
Sourced in United States
Testosterone cypionate is a synthetic form of the male hormone testosterone. It is a laboratory product used in scientific research and experimentation. The core function of testosterone cypionate is to provide a stable and controlled source of testosterone for various investigational and analytical purposes.
Automatically generated - may contain errors
Lab products found in correlation
Nandrolone decanoate, by Steraloids (5 mentions)
Boldenone undecylenate, by Steraloids (4 mentions)
Arginine vasopressin, by Merck Group (2 mentions)
Eticlopride hydrochloride, by Merck Group (2 mentions)
Serotonin hydrochloride, by Merck Group (1 mentions)
Arginine vasopressin avp, by Merck Group (1 mentions)
Bicuculine, by Merck Group (1 mentions)
Muscimol, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Enzalutamide, by Selleck Chemicals (1 mentions)
Matrigel, by BD (1 mentions)
Sesame oil vehicle, by Merck Group (1 mentions)
17α methyltestosterone, by Steraloids (1 mentions)
7 protocols using testosterone cypionate
1
Neuroendocrine Compound Preparation
2
Anabolic Steroids Administration Protocol
3
Anabolic Hormones and Vasopressin Regulation
4
Anabolic Steroid and GABAergic Protocol
5
In Vitro Oocyte Maturation and Fertilization
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All chemicals were purchased from Millipore Sigma (St. Louis, MO, USA) unless otherwise indicated. Testosterone cypionate was from Steraloids (Newport, RI, USA) and was prepared as an 8 mg/ml solution in sesame oil. Equine chorionic gonadotropin (eCG) was from Calbiochem. The medium for oocyte collection was HEPES-buffered MEMα (Gibco 12000022, Thermo Fisher, Waltham, MA, USA) containing penicillin, streptomycin, and polyvinyl alcohol (PVA), and 10 µM milrinone to prevent spontaneous meiotic maturation (Mehlmann et al., 2019) . For overnight oocyte maturation, oocytes were washed into bicarbonate-buffered MEMα (Mehlmann et al., 2019) containing 5% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) without milrinone. For in vitro fertilization, cumulus masses were collected in human tubal fluid medium (HTF; Cook Medical Inc. IVF medium (#K-RVFE; Fisher) containing reduced glutathione. Sperm were capacitated in IVF medium (Mehlmann and Kline, 1994) containing 15 mg/ml Fraction V bovine serum albumin.
6
Modeling Castrated Prostate Cancer in Mice
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Male NSG mice aged 8–12 weeks were obtained from the Sidney Kimmel Comprehensive Cancer Center Animal Core Facility and surgically castrated. SKCaP PDX tissue was minced, mixed with Matrigel (BD Biosciences) and implanted subcutaneously on the flank. Testosterone was administered by implantation of a slow-release subcutaneous pellet in the opposite flank. Pellets were assembled using 25 mm sections of Silastic Laboratory tubing, filled with 30 mg testosterone cypionate (Steraloids), sealed on both ends with Silastic Medical Adhesive Type A, then sterilized. Enzalutamide (SelleckChem) was administered by oral gavage 10 mg/kg/day in 200 μL 1% carboxymethyl cellulose, 0.1% Tween-80, 5% DMSO. Tumors were measured twice weekly using microcalipers, and tumor volume was calculated using the following formula: 0.5236 × L × W × H. At study completion, the mice were euthanized and the tumors were extracted. Tumors were flash frozen for subsequent lysis for immunoblot analyses and formalin-fixed for subsequent IHC analyses. All mice were housed in the Johns Hopkins animal facility.
7
Anabolic Steroids Preparation and Storage
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Testosterone cypionate, 17α-methyltestosterone, and nandrolone decanoate were purchased from Steraloids (Newport, RI). Sesame oil vehicle was purchased from Sigma. A fresh cocktail of all the three AAS in Sesame oil vehicle was made every week.
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